Proteins of tooth pulp separated by 2D electrophoresis (excision) and stained with silver.
Main topics:
- developing of new separation methods (capillary electromigration techniques), for example:
- affinity electromigration methods
- application of gold nanoparticles in capillary electromigration methods
- forensic application (including coupling CE-MS)
- analysis of connective tissues - proteomic and physiological analysis of biological samples when many new proteins were identified, for example:
- proteom of human teeth in connection to decoy
- insoluble proteins from the avian eggshell, especially cuticle
- analysis of posttraslational modifications of proteins
- determination of arising compounds in glycation reaction as well as sites of modifications
- identification of age-related changes in proteins – oxidation and deamidation of proteins
- analysis of a broad spectrum of biologically important compounds in cooperation with many departments of our Institute, various Czech Institutes as well as international cooperation (e.g. proteins, steroids, pigments)
- proteomic facility of Institute of Physiology CAS
Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence)
CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100)
Hewlett-Packard (Agilent) HPLC/MSD 1100 System. Detectors: DAD, fluorescence, mass spectrometer (quadrupole), radioactive and evaporative light-scattering (Polymer Labs.). Agilent LC-MSD Trap XCT-ultra Proteomic software (SpectrumMill).
