Staňková, H., Valárik, M., Lapitan, N.L.V., Berkman, P.J., Batley, J., Edwards, D., Luo. M.-Ch., Tulpová, Z., Kubaláková, M., Stein, N., Doležel, J., Šimková, H.
THEORETICAL AND APPLIED GENETICS
128:
1373-1383,
2015
Keywords:
Abstract:
Key message Making use of wheat chromosomal
resources, we developed 11 gene-associated markers
for the region of interest, which allowed reducing gene
interval and spanning it by four BAC clones.
Abstract Positional gene cloning and targeted marker
development in bread wheat are hampered by high complexity
and polyploidy of its nuclear genome. Aiming
to clone a Russian wheat aphid resistance gene Dn2401
located on wheat chromosome arm 7DS, we have developed
a strategy overcoming problems due to polyploidy
and enabling efficient development of gene-associated
markers from the region of interest. We employed information
gathered by GenomeZipper, a synteny-based tool
combining sequence data of rice, Brachypodium, sorghum
and barley, and took advantage of a high-density linkage
map of Aegilops tauschii. To ensure genome- and locusspecificity
of markers, we made use of survey sequence
assemblies of isolated wheat chromosomes 7A, 7B and
7D. Despite the low level of polymorphism of the wheat D
subgenome, our approach allowed us to add in an efficient
and cost-effective manner 11 new gene-associated markers
in the Dn2401 region and narrow down the target interval
to 0.83 cM. Screening 7DS-specific BAC library with the
flanking markers revealed a contig of four BAC clones that
span the Dn2401 region in wheat cultivar ‘Chinese Spring’.
With the availability of sequence assemblies and GenomeZippers
for each of the wheat chromosome arms, the proposed
strategy can be applied for focused marker development
in any region of the wheat genome.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková,
Helena Toegelová ...,
Zuzana Tulpová,
Miroslav Valárik
