Intranet Čeština

Membrane Transport

Membrane Transport Sodium and potassium transporting protein observed in yeast cells through a fluorescence microscope. Green fluorescence corresponds to the protein localized in the cell plasma membrane; red fluorescence visualizes membranes of vacuoles.

We study the proteins transporting compounds and signals across the cell membranes. These proteins, called transporters, assure the uptake of nutrients into the cells, efflux of waste compounds from cells and communication with the environment.  To study the animal and plant transporters, we mainly use a model eukaryotic microorganism – yeasts. Our research is focused mainly on:

  • Structure, function and regulation of cell transport systems at protein molecule level.
  • Role of transporters in specific cell properties and in diseases.
  • Role of transporters in Candida virulence and pathogenicity.
  • Development of new techniques to estimate cell physiological parameters.

Projects

Cell cation and pH homeostasis

The intracellular concentration of potassium and sodium cations, as well as of protons, is strictly regulated via the activity of a series of membrane proteins that mediate the flux of cations and protons with various transport mechanisms. Incorrect functioning of some transporters results in serious disorders and diseases. We study in detail the roles of individual transporters and the impact of their activity on cell fitness. More

Transporters reflected in specific properties of nonconventional yeasts

Some yeast species survive extreme changes in the environmental pH, temperature or osmotic pressure. We aim to identify and characterize specific transporters whose activity contributes to the ability to survive adverse environmental conditions. Acquired knowledge will help to improve the properties of yeast species used in industrial processes. More

Transporters reflected in specific properties of nonconventional yeasts

Some yeast species survive extreme changes in the environmental pH, temperature or osmotic pressure. We aim to identify and characterize specific transporters whose activity contributes to the ability to survive adverse environmental conditions. Acquired knowledge will help to improve the properties of yeast species used in industrial processes. More

Achievements

The best poster award for Klara Richerova

Klara Richterova is the author of award-winning poster at 6th Czech-Swiss symposium BioTech 2014. The work with name „Heterologous expression of three laccases from different source of origin in yeast Saccharomyces cerevisiae and their use for environmental application.“ was born in collaboration with Institute of Organic Chemistry and Biochemistry AS CR. Congratulation!  More

Publications

Antošová, Zuzana - Sychrová, Hana . Yeast Hosts for the Production of Recombinant Laccases: A Review . Molecular Biotechnology. 2016, roč. 58, 2, p. 93-116 . IF = 1.752 [ASEP] [ doi ]
Elicharová, Hana - Hušeková, Barbora - Sychrová, Hana . Three Candida albicans potassium uptake systems differ in their ability to provide Saccharomyces cerevisiae trk1trk2 mutants with necessary potassium . FEMS Yeast Research. 2016, roč. 16, 4, fow039 . IF = 2.479 [ASEP] [ doi ]
Llopis-Torregrosa, Vincent - Hušeková, Barbora - Sychrová, Hana . Potassium Uptake Mediated by Trk1 Is Crucial for Candida glabrata Growth and Fitness . PLoS ONE. 2016, roč. 11, 4, e0153374 . IF = 3.057 [ASEP] [ doi ]
Hušeková, Barbora - Elicharová, Hana - Sychrová, Hana . Pathogenic Candida species differ in the ability to grow at limiting potassium concentrations . Canadian Journal of Microbiology. 2016, roč. 62, 5, p. 394-401 . IF = 1.335 [ASEP] [ doi ]
Kodedová, Marie - Sychrová, Hana . High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species . Journal of Biotechnology. 2016, roč. 233, Sep 10, p. 26-33 . IF = 2.667 [ASEP] [ doi ]

Article photogallery

Kurz CBV Funkce a struktura buněčných membrán CBV course Cell membrane function and structure
Účast oddělení na konferenci 30th International Specialized Symposium on Yeast (ISSY), Stará Lesná, Slovensko, červen 2013 Department participated in the 30th International Specialized Symposium on Yeast (ISSY), Stará Lesná, Slovakia, June 2013
Novoroční oběd New Year's lunch
Novoroční oběd New Year's lunch
Novoroční oběd New Year's lunch
Loučení s italskou stážistkou Melissou Bizzarri Farewell party of visiting student Melissa Bizzarri
Návštěva Dr. Omara Pantoja na našem oddělení. Dr. Omar Pantoja visited our department.
Manipulace s kvasikami uloženými v -80 °C Yeasts in -80 °C
Měření objemu kvasinkových buněk Cell volume measurement.
Naše laboratoře před a po rekonstrukci One of our lab before and after reconstruction
Naše laboratoře před a po rekonstrukci One of our lab before and after reconstruction
Naše laboratoře před a po rekonstrukci One of our lab before and after reconstruction
Příprava kapkového testu Preparation of drop test
Kapkový test po několika dnech v inkubátoru Drop test after several days in incubator
Světelná mikroskopie Microscopy
Inokulace média kvasinkovými buňkami Inoculation of culture media
Inokulace média kvasinkovými buňkami Inoculation of culture media
V plném pracovním nasazení Busy time
V plném pracovním nasazení Busy time
Třepačka - inkubátor tekutých kultur Incubator of liquid cultures
The entire photogallery

Contacts

Fyziologický ústav AV ČR, v.v.i.
Department of Membrane Transport
Vídeňská 1083
14220 Praha 4
tel. +420 241 062 667
fax. +420 241 062 284
 

sychrova@fgu.cas.cz

People

Hana Sychrová, DrSc.
head of the department
Olga Zimmermannová, Ph.D. (née Kinclová)
deputy head of department
Zuzana Antošová, Ph.D. (née Chrastilová)
postdoc
  Marie Kodedová, Ph.D.
postdoc
Klára Papoušková, Ph.D. (née Velková)
postdoc

Vicent Liopis-Torregrosa, PhD.

postdoc

Hana Čuláková, PhD.
postdoc
Michal Růžička, Ph.D.
lab manager
Michala Dušková, Ph.D. (née Bubnová)
postdoc
Hana Elicharová
PhD student

Klára Herkommerová (née Richterová)

PhD student

Kristina Felcmanová

PhD student
Denis Cmunt
pregradual student

Anna-Marie Makarova

pregradual student

Michaela Hrášková

pregradual student
Pavla Herynková
lab technician

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