Terracciano, I., Maccaferri, M., Bassi, F., Mantovani, P., Sanguineti, M.C., Salvi, S., Šimková, H., Doležel, J., Massi, A., Ammar, K., Kolmer, J., Tuberosa, R.
THEORETICAL AND APPLIED GENETICS
126:
1077-1101,
2013
Keywords:
Abstract:
Leaf rust (Puccinia triticina Eriks. & Henn.) is
a major disease affecting durum wheat production. The
Lr14a-resistant gene present in the durum wheat cv. Creso
and its derivative cv. Colosseo is one of the best characterized
leaf-rust resistance sources deployed in durum
wheat breeding. Lr14a has been mapped close to the simple
sequence repeat markers gwm146, gwm344 and wmc10 in
the distal portion of the chromosome arm 7BL, a genedense
region. The objectives of this study were: (1) to
enrich the Lr14a region with single nucleotide polymorphisms
(SNPs) and high-resolution melting (HRM)-based
markers developed from conserved ortholog set (COS)
genes and from sequenced Diversity Array Technology
(DArT) markers; (2) to further investigate the gene content
and colinearity of this region with the Brachypodium
and rice genomes. Ten new COS-SNP and five HRM
markers were mapped within an 8.0 cM interval spanning
Lr14a. Two HRM markers pinpointed the locus in an
interval of \1.0 cM and eight COS-SNPs were mapped
2.1–4.1 cM distal to Lr14a. Each marker was tested for its
capacity to predict the state of Lr14a alleles (in particular,
Lr14-Creso associated to resistance) in a panel of durum
wheat elite germplasm including 164 accessions. Two of
the most informative markers were converted into KASPar
markers. Single assay markers ubw14 and wPt-4038-
HRM designed for agarose gel electrophoresis/KASPar
assays and high-resolution melting analysis, respectively,
as well as the double-marker combinations ubw14/ubw18,
ubw14/ubw35 and wPt-4038-HRM–ubw35 will be useful
for germplasm haplotyping and for molecular-assisted
breeding.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel,
Hana Šimková
