Curriculum vitae:
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2011
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Postdoc; Laboratory of Fish Genetics, IAPG AS CR, v.v.i.
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2011
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Postdoc; Department of Ecology, Faculty of Science, Charles University in Prague
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2007 – 2011
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Ph.D.; Department of Zoology, Faculty of Science, Charles University in Prague and Laboratory of Fish Genetics, IAPG AS CR, v.v.i., Ph.D. thesis: „Evolution of sex-determining mechanisms and genomes in Squamate reptiles (Reptilia: Squamata)“
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2009
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Dr.; Faculty of Science, Charles University in Prague
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2002 – 2007
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M.Sc.; Faculty of Science, Charles University in Prague; M.Sc. thesis: ”Karyotypic evolution in eye-lid geckos (Squamata: Eublepharidae), a group with different modes of sex-determination.”
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1999 – 2006
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laboratory assistant; Department of Histology and Embryology, 1st Faculty of Medicine, Charles University
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Specilalization:
The main interest of our research is evolution of sex-determining mechanisms. Particular mode of sex determination is important not only at the individual organismal level (i.e. establishment of the sex during ontogeny), but it can also influence population sex ratio and therefore total evolvability and susceptibility to extinction of the lineage in question. We are predominantly focused on reptiles (Sauropsida) as a model group for our research because in general we can find large variability in sex-determining mechanisms in this group. In some lineages sex is determined by sex chromosomes (XX/XY or ZZ/ZW) and in others by environmental factors (such as temperature). We study evolution of sex determining mechanisms and also evolution of genome organisation across lineages of reptiles and for larger comparison also in other vertebrates using various technics of classical and molecular cytogenetics including fluorescent in situ hybridization, laser microdissection or chromosome sorting on flow cytometer.
Internship abroad:
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2008, 2009, 2010, 2011
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Molecular Cytogenetics Laboratory, Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, UK (10 months in total)
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2009
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CIBIO – Research Centre in Biodiversity and Genetic Resources, Porto –Vairao, Portugal (three weeks)
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2008
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Department Systematics and Evolution, Muséum National d’Histoire Naturelle, Paris, Frace (two weeks)
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Scientific collaboration:
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Willem Rens, Malcolm Ferguson-Smith
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Department of Veterinary Medicine, University of Cambridge, UK
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Eduard Kejnovský
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Institute of Biofysics, AS CR, v.v.i., Brno, Czech Republic
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Massimo Giovannoti, Vincenzo Caputo, Ettore Olmo
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Università Politecnica delle Marche, Ancona, Italy
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Vladimir Trifonov
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Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia
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Karen Ventura
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Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil
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Teaching activities:
“Genome Evolution” Master’s course, Department of Ecology, Charles University in Prague
Co-supervisor of two PhD. and one BSc. students at the Department of Ecology, Charles University in Prague; co-supervisor of two previous defended bachelor and two master theses
Grants:
In the past and recently: principle investigator of 4 projects (Grant Agency of Charles University, Thermal adaptation in ectotherms - European Science Foundation, Synthesys); member of the research team of 3 projects (Czech Science Foundation, Grant Agency of Charles University)
Publications:
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Johnson Pokorná M, Kratochvíl L (2014) What was the ancestral sex-determining mechanism in amniote vertebrates? Biological Reviews DOI: 10.1111/brv.12156
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Koubová M, Johnson Pokorná M, Rovatsos M, Farkačová K, Altmanová M, Kratochvíl L (2014) Sex determination in madagascar geckos of the genus Paroedura (Squamata: Gekkonidae): are differentiated sex chromosomes indeed so evolutionary stable? Chromosome Research 22: 441-452
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Rovatsos M, Altmanová M, Johnson Pokorná M, Kratochvíl L (2014) Novel X-linked genes revealed by qPCR in the Green Anole, Anolis carolinensis. G3: Genes Genomes Genetics 114.014084
http://www.g3journal.org/content/4/11/2107.full.pdf+html
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Johnson Pokorná M, Rovatsos M, Kratochvíl L (2014) Sex chromosomes and karyotype of the (nearly) mythical creature, the Gila Monster, Heloderma suspectum (Squamata: Helodermatidae). Plos One 9 e104716
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0104716
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Pokorná M, Altmanová M, Kratochvíl L (2014) Multiple sex chromosomes in the light of female meiotic drive in amniote vertebrates. Chromosoe Research 22: 35-44
http://link.springer.com/article/10.1007/s10577-014-9403-2#page-1
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Pokorná M, Rens W, Rovatsos M, Kratochvíl L (2014) A ZZ/ZW sex chromosome system in the Thick-Tailed Gecko (Underwoodisaurus milii; Squamata: Gekkota: Carphodactylidae), a member of the ancient gecko lineage. Cytogenetic and Genome Research 142: 190-196
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Rovatsos M, Altmanová M, Pokorná M, Kratochvíl L (2014) Conserved sex chromosomes across adaptively radiated Anolis lizards. Evolution 68: 2079-2085
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Rovatsos M, Pokorná M, Altmanová M, Kratochvíl L (2014) Cretaceous park of sex determination: sex chromosomes are conserved across iguanas. Biology Letters 10: 20131093
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2012
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Pokorná M, Giovannotti M, Kratochvíl L, Caputo V, Olmo E, Ferguson-Smith MA, Rens W. Conservation of chromosomes syntenic with avian autosomes in squamate reptiles revealed by comparative chromosome painting. Chromosoma 121: 409-418
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2011
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Pokorná M, Kratochvíl L, Kejnovský E. Microsatellite distribution on sex chromosomes at different stages of heteromorphism and heterochromatinization in two lizard species (Squamata: Eublepharidae: Coleonyx elegans and Lacertidae: Eremias velox). BMC Genetics 12: 91 http://www.biomedcentral.com/content/pdf/1471-2156-12-90.pdf
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2011
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Pokorná M, Giovannotti M, Kratochvil L, Kasai F, Trifonov VA, O'Brien PCM, Caputo V, Olmo E, Ferguson-Smith MA, Rens W. Strong conservation of the bird Z chromosome in reptilian genomes is revealed by comparative painting despite 275 My divergence. Chromosoma 120: 455-468
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2010
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Pokorná M, Rábová M, Ráb P, Ferguson-Smith MA, Rens W, Kratochvíl L. Differentiation of sex chromosomes and karyotypic evolution in the eye-lid geckos (Squamata: Gekkota: Eublepharidae), a group with different modes of sex determination. Chromosome Research 18: 809–820
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2009
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Pokorná M, Kratochvíl L. Phylogeny of sex-determining mechanisms in squamate reptiles: Are sex chromosomes an evolutionary trap? Zoological Journal of the Linnean Society 156: 168-183
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