Analytical Chemistry

Václav Kašička

The thesis will be focused on the development of new high-performance electromigration methods, capillary affinity electrophoresis and open-tubular capillary electrochromatography, and on their application to the investigation of the non-covalent interactions of biologically active peptides, proteins, nucleosides, nucleotides and other biomolecules with low- and high-molecular-mass ligands or receptors. New procedures will be developed for quantitative characterization of both weak and strong complexes of biomolecules in a free solution and on the interface of the liquid and solid phases. Interactions of biomolecules, e.g. peptide hormones or drugs with receptors, enzymes with substrates and inhibitors, and ionophores with small ions in a free solution will be investigated by different modes of capillary affinity electrophoresis. Interactions of biomolecules on the interface of solid and liquid phases will be studied by open tubular capillary electrochromatography with ligands or receptors immobilized on the inner wall of the fused silica capillary or on the gold nanoparticles in the mobile phase. Strength of the interactions of biomolecules will be quantified by the stability constants of their complexes.