Organic Chemistry

Tutor	Doc. Ing. Michal Hocek, CSc. DSc.
Team	Michal Hocek
Topic	Synthesis of fluorescently-labelled nucleoside triphosphates and enzymatic construction of labelled nucleic acids for the study of their interactions with proteins
Faculty	FCHT / FPBT VŠCHT, PřF UK
Abstract	Novel modified nucleoside triphosphates will be prepared and their polymerase catalyzed incorporations to oligonucleotides and DNA will be studied. The base-modifications will focus on environment-selective fluorescent groups (solvatochromic fluorophores, molecular rotors). Interactions of these modified DNAs with selected DNA-binding proteins (e.g. restriction endonucleases, DNA methyl-transferases, transcription factors etc.) will be studied. Leading references: 1. Riedl, J.; Pohl, R.; Rulíšek, L.; Hocek, M. "Synthesis and Photophysical Properties of Biaryl-Substituted Nucleos(t)ides. Polymerase Synthesis of DNA Probes Bearing Solvatochromic and pH Sensitive Dual Fluorescent and 19F NMR Labels" J. Org. Chem. 2012, 77, 1026-1044. 2. Riedl, J.; Pohl, R.; Ernsting, N. P.; Orsag, P.; Fojta, M.; Hocek M. "Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions" Chem. Sci. 2012, 3, 2797-2806. 3. Riedl, J.; Ménová, P.; Pohl, R.; Orság, P.; Fojta, M.; Hocek, M. "GFP-like fluorophores as DNA labels for studying DNA-protein interactions" J. Org. Chem. 2012, 77, 8287-8293.