Mayer, K.F.X., Martis, M., Hedley, P.E., Šimková, H., Liu, H., Morris, J.A., Steuernagel, B., Taudien, S., Roessner, S., Gundlach, H., Kubaláková, M., Suchánková, P.,Murat, F., Felder, M., Nussbaumer, T., Graner, A., Salse, J., Endo, T.R., Sakai, H., Tanaka, T., Itoh, T., Sato, K., Platzer, M., Matsumoto, T., Scholz, U., Doležel, J., Waugh, R., Stein, N.
PLANT CELL 23: 1249-1263, 2011

Klíčová slova:
Abstrakt: We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign ;86% of the estimated ;32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
DOI:
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Autoři z ÚEB: Jaroslav Doležel, Hana Šimková, Pavla Suchánková