11.1. 2016, Membránový transport

PhD Project1:

Structure and function of proteins transporting alkali metal cations

 

Research topics:                       

The aim of the PhD thesis is to identify amino-acid residues of alkali-metal-cation transporters which are involved in the regulation of transporter’s activity, substrate specificity and in its tertiary structure. The work will involve cloning of genes, random and site-directed mutagenesis, heterologous expression of transporters in yeast cells, characterization of their localization and transport activity.

Candidate’s profile (requirements):

Candidate’s profile (requirements): Biochemistry, microbiology, cell or molecular biology background, basic laboratory (PCR, DNA electrophoresis, bacteria transformation, plasmid isolation) and bioinformatics (DNA and protein sequence search and comparison, sequence analysis, plasmid and primers design) techniques.

Supervisor:  RNDr. Hana Sychrová, DrSc.

 

PhD Project2:

Biogenesis and degradation of yeast transport systems

 

Research topics:                       

The aim of this PhD thesis will be the identification and characterization of so far unknown proteins that participate in biogenesis, degradation and activity regulation of transporters involved in alkali-metal-cation and pH homeostasis of yeast cells. The work will consist of ORF-library screens, construction of knock-out strains, and their phenotype and physiological parameters characterization.

Candidate’s profile (requirements):

Candidate’s profile (requirements): Biochemistry, microbiology, cell or molecular biology background, basic laboratory (PCR, DNA electrophoresis, bacteria transformation, plasmid isolation) and bioinformatics (DNA and protein sequence search and comparison, sequence analysis, plasmid and primers design) techniques.

Supervisor:  RNDr. Hana Sychrová, DrSc.