Sodium and potassium transporting protein observed in yeast cells through a fluorescence microscope. Green fluorescence corresponds to the protein localized in the cell plasma membrane; red fluorescence visualizes membranes of vacuoles.
We study the proteins transporting compounds and signals across the cell membranes. These proteins, called transporters, assure the uptake of nutrients into the cells, efflux of waste compounds from cells and communication with the environment. To study the animal and plant transporters, we mainly use a model eukaryotic microorganism – yeasts. Our research is focused mainly on:
- Structure, function and regulation of cell transport systems at protein molecule level.
- Role of transporters in specific cell properties and in diseases.
- Role of transporters in Candida virulence and pathogenicity.
- Development of new techniques to estimate cell physiological parameters.
Projects
The intracellular concentration of potassium and sodium cations, as well as of protons, is strictly regulated via the activity of a series of membrane proteins that mediate the flux of cations and protons with various transport mechanisms. Incorrect functioning of some transporters results in serious disorders and diseases. We study in detail the roles of individual transporters and the impact of their activity on cell fitness.
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Some of the transporters existing in the membranes of pathogenic Candida species differ by its structure and activity from those of the host organism. These transporters thus may serve as targets for the development of new antifungal drugs that will affect yeast cells but not cells of the host.
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Some yeast species survive extreme changes in the environmental pH, temperature or osmotic pressure. We aim to identify and characterize specific transporters whose activity contributes to the ability to survive adverse environmental conditions. Acquired knowledge will help to improve the properties of yeast species used in industrial processes.
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Achievements
Olga Zimmermannová, Ph.D. gained a grant project from The Czech Science Foundation (GA CR 17-01953S): Physiological role of cation/proton antiporters in monovalent cation homeostasis, Ca2+ signalling and intracellular pH regulation
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One of our latest papers characterizes a novel type of yeast transporters that we have named Vhc1.
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Klara Richterova is the author of award-winning poster at 6th Czech-Swiss symposium BioTech 2014. The work with name „Heterologous expression of three laccases from different source of origin in yeast Saccharomyces cerevisiae and their use for environmental application.“ was born in collaboration with Institute of Organic Chemistry and Biochemistry AS CR. Congratulation!
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Publications
Rosas-Santiago, P. - Zimmermannová, Olga - Vera-Estrella, R. - Sychrová, Hana - Pantoja, O.
Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter
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Biochimica Et Biophysica Acta-Biomembranes. 2016, roč. 1858, 1, p. 67-74
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IF = 3.687
[ASEP]
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doi
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Pérez-Torrado, R. - Oliveira, B. M. - Zemančíková, Jana - Sychrová, Hana - Querol, A.
Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress
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Frontiers in Microbiology. 2016, roč. 7, Mar 31, p. 435
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IF = 4.165
[ASEP]
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doi
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Llopis-Torregrosa, Vincent - Hušeková, Barbora - Sychrová, Hana
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Potassium Uptake Mediated by Trk1 Is Crucial for Candida glabrata Growth and Fitness
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PLoS ONE. 2016, roč. 11, 4, e0153374
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IF = 3.057
[ASEP]
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doi
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Kodedová, Marie - Sychrová, Hana
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High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species
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Journal of Biotechnology. 2016, roč. 233, Sep 10, p. 26-33
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IF = 2.667
[ASEP]
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doi
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Hušeková, Barbora - Elicharová, Hana - Sychrová, Hana
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Pathogenic Candida species differ in the ability to grow at limiting potassium concentrations
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Canadian Journal of Microbiology. 2016, roč. 62, 5, p. 394-401
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IF = 1.335
[ASEP]
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doi
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CBV course Cell membrane function and structure
Department participated in the 30th International Specialized Symposium on Yeast (ISSY), Stará Lesná, Slovakia, June 2013
New Year's lunch
New Year's lunch
New Year's lunch
Farewell party of visiting student Melissa Bizzarri
Dr. Omar Pantoja visited our department.
Yeasts in -80 °C
Cell volume measurement.
One of our lab before and after reconstruction
One of our lab before and after reconstruction
One of our lab before and after reconstruction
Preparation of drop test
Drop test after several days in incubator
Microscopy
Inoculation of culture media
Inoculation of culture media
Busy time
Busy time
Incubator of liquid cultures
