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The role of intestine in differential metabolic effects of various lipid forms of dietary omega-3 fatty acids


Long-chain fatty acids of n-3 series (omega-3), mainly EPA and DHA of marine origin, may protect against obesity and exert hypolipidemic effects. While the mechanisms of omega-3’s effects on lipid metabolism in the liver are relatively well described, changes in metabolism in the intestine due to omega-3 are not entirely understood. Previous studies showed that metabolic effects are stronger when omega-3 are administered as phospholipids (Rossmeisl et al., PlosOne 2012). The main goal of this project is to identify the nature of differential metabolic effects of omega-3 depending on the lipid form of their administration (triacylglycerols, phospholipids or wax esters), mainly with regard to the involvement of the intestine in these effects.

 

Experiments will be performed in mice under obesogenic conditions induced by high-fat feeding, with the following specific aims:

1. to assess the bioavailability of EPA and DHA (omega-3 index) at the level of intestine as well as in plasma and major insulin-sensitive tissues involved in omega-3 action;

2. to identify the most regulated cellular processes that are affected in the intestine by dietary interventions with novel omega-3 concentrates;

3. to compare lipid transport capacity and chylomicron secretion in mice previously fed high-fat diets supplemented with different lipid forms of omega-3;

4. to reveal the potency of chronic dietary intake of omega-3 with regard to modulation of endocrine function of the gastrointestinal system.

 

 Supported by the CSF project no. 16-08124S (2016-2018, PI: Olga Horakova PhD., IPHYS).

© 2014 INSTITUTE OF PHYSIOLOGY CAS