Physical Chemistry

The aim of the thesis will be to develop new high-performance electromigration methods, capillary affinity electrophoresis and open-tubular capillary electrochromatography, and their application to the investigation of the interactions of biologically active peptides, proteins, nucleosides, nucleotides and other biomolecules with low- and high-molecular-mass ligands or receptors. New procedures will be developed for quantitative characterization of both weak and strong interactions of biomolecules in a free solution and on the interface of the liquid and solid phases. Interactions of biomolecules, e.g. peptide hormones or drugs with receptors, enzymes with substrates and inhibitors, and ionophores with small ions in a free solution will be investigated by different modes of capillary affinity electrophoresis. Interactions of biomolecules on the interface of solid and liquid phases will be studied by open tubular capillary electrochromatography with ligands or receptors immobilized on the inner capillary wall or on the nanoparticles in the mobile phase. Strength of the interactions of biomolecules will be quantified by the stability constants of their complexes.