Intranet Čeština

Biomathematics

Biomathematics

The Department of Biomathematics consists of two research groups: (1) Bioimaging and Image Analysis, and (2) Biochemistry of Membrane Receptors. Operating mainly at in situ and in vitro levels, respectively, the two groups complement each other when studying phenomena confined to the cell membrane.

 

1.      Bioimaging and Image Analysis

The group conducts research into the 3D microanatomical aspects of physiological phenomena at mesoscopic, microscopic and ultrastructural level. It is engaged in a range of collaborative projects across the campus and beyond. If interested please contact us (jiri.janacek@fgu.cas.cz).

 

CURRENTLY FUNDED PROJECTS

  •  Brain development in birds, as relevant to the ability to fly
  •  The conduction system of developing murine heart

 

LONG-TERM PROJECTS

  • Development of 3D image analysis and stereological methods including software (employed, e.g., in a volumetric analysis of Langerhans islets from human donors)
  • Microscopic optical-contrasting (label-free) strategies to visualize living cells and tissues

 

OTHER PROJECTS (selection only)

  • Effects of ionizing radiation on blood capillaries network
  • Effects of elevated CO2 on the photosynthetic apparatus’ performance and  ultrastructure
  • Mechanisms governing the formation of extracellular matrix proteins (collagen and elastin)

 

IN-HOUSE IMAGING MODALITIES

  • Laser scanning confocal microscopy
  • Two-photon excitation microscopy
  • Second harmonic generation (SHG) microscopy
  •  ‘White-light‘ laser and hybrid detectors (on Leica TCS SP8 WLL MP microscope)
  • FRAP recording, FRET analysis, FLIM/PLIM recording including λ2 scans 
  • Optical projection tomography (OPT)
  • Selective plane illumination (light sheet) microscopy (SPIM)
  • Phase-, modulation- and interference contrast microscopy

 

AVAILABLE SOFTWARE AND PROTOCOLS

  • Software for 3D image analysis, stereology, spatial statistics and deconvolution (Hyughens)
  • Optical/chemical clearing protocols for OPT and SPIM

 

OPEN ACCESS TO EQUIPMENT

The group is part of the Czech BioImaging infrastructure [1] supporting open access to most of its microscopes (listed above), and implicitly also to its expertise. The portfolio includes, among other imaging equipment located at other departments, a Nipkow spinning-disk (“Carv II“) confocal microscope [2].

[1]      http://www.czech-bioimaging.cz/

[2]      http://www.fgu.cas.cz/en/articles/529-czech-bioimaging-2016-2019

 

 

2.      Biochemistry of Membrane Receptors

Our research focuses on the cellular and molecular mechanisms of desensitization of hormone response mediated by the activation of G protein coupled receptors (GPCR). G protein coupled receptors are plasma membrane integral proteins which serve as transducers of extracellular signals across the plasma membrane bilayer to the cell interior. GPCR play a key role in the regulation of many physiological processes and functions. Moreover, GPCRs represent one of the most important groups of targets for therapeutics.

 

The main areas of the research are:

  • The role of cell membrane and membrane domains
  • Opioid receptors and drug addiction
  • The effect of monovalent ions on δ-opioid receptors – analysis of lithium effect in living cells and isolated cell membranes
  • GABA-B - signalling cascades

 

Detail informations here.

Projects

Achievements

Publications

Ziegler, A. - Neues, F. - Janáček, Jiří - Beckmann, F. - Epple, M. Mineral in skeletal elements of the terrestrial crustacean Porcellio scaber: SR mu CT of function related distribution and changes during the moult cycle . Arthropod Structure & Development. 2017, roč. 46, 1, p. 63-76 . IF = 1.546 [ASEP] [ doi ]
Vošahlíková, Miroslava - Ujčíková, Hana - Chernyavskiy, Oleksandr - Brejchová, Jana - Roubalová, Lenka - Alda, M. - Svoboda, Petr . Effect of therapeutic concentration of lithium on live HEK293 cells; increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane . Biochimica et Biophysica Acta. General Subjects. 2017, roč. 1861, 5, p. 1099-1112 . IF = 4.702 [ASEP] [ doi ]
Techniková, L. - Tunák, M. - Janáček, Jiří . New objective system of pilling evaluation for various types of fabrics . Textile Institute. Journal. 2017, roč. 108, 1, p. 123-131 . IF = 1.007 [ASEP] [ doi ]
Ošťádal, M. - Lišková, Jana - Hadraba, Daniel - Eckhardt, Adam . Possible Pathogenetic Mechanisms and New Therapeutic Approaches of Pes Equinovarus . Physiological Research. 2017, roč. 66, 3, p. 403-410 . IF = 1.461 [ASEP]
Lišková, Jana - Hadraba, Daniel - Filová, Elena - Koňařík, M. - Pirk, J. - Jelen, K. - Bačáková, Lucie . Valve interstitial cell culture: Production of mature type I collagen and precise detection . Microscopy Research and Technique. 2017, roč. 80, 8, p. 936-942 . IF = 1.147 [ASEP] [ doi ]
Jirák, D. - Janáček, Jiří . Volume of the crocodilian brain and endocast during ontogeny . PLoS ONE. 2017, roč. 12, 6, článku e0178491 . IF = 2.806 [ASEP] [ doi ]
Czerneková, Michaela - Jönsson, K. I. - Chajec, Ł. - Student, S. - Poprawa, I. The structure of the desiccated Richtersius coronifer (Richters, 1903) . Protoplasma. 2017, roč. 254, 3, p. 1367-1377 . IF = 2.870 [ASEP] [ doi ]
Bačáková, Markéta - Pajorová, Julie - Stránská, D. - Hadraba, Daniel - Lopot, F. - Riedel, Tomáš - Brynda, Eduard - Žaloudková, Margit - Bačáková, Lucie . Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells . International Journal of Nanomedicine. 2017, roč. 12, Feb, p. 1143-1160 . IF = 4.300 [ASEP] [ doi ]
Czerneková, Michaela - Jönsson, K. I. Mitosis in storage cells of the eutardigrade Richtersius coronifer . Zoological Journal of the Linnean Society. 2016, roč. 178, 4, p. 888-896 . IF = 2.711 [ASEP] [ doi ]
Czerneková, Michaela - Jönsson, K. I. Experimentally Induced Repeated Anhydrobiosis in the Eutardigrade Richtersius coronifer . PLoS ONE. 2016, roč. 11, 11, článku e0164062 . IF = 2.806 [ASEP] [ doi ]
Pearce, P. - Brownbill, P. - Janáček, Jiří - Jirkovská, M. - Kubínová, Lucie - Chernyavsky, I. L. - Jensen, O. E. Image-Based Modeling of Blood Flow and Oxygen Transfer in Feto-Placental Capillaries . PLoS ONE. 2016, roč. 11, 10, článku e0165369 . IF = 2.806 [ASEP] [ doi ]
Kohutiar, Matej - Ivica, Josko - Vytášek, R. - Skoumalová, A. - Illner, Jan - Šantorová, P. - Wilhelm, Jiří . Comparison of the effects of tert-butyl hydroperoxide and peroxynitrite on the oxidative damage to isolated beef heart mitochondria . Physiological Research. 2016, roč. 65, 4, p. 617-626 . IF = 1.461 [ASEP]
Filová, Eva - Jakubcová, Barbora - Danilová, I. - Kuželová Košťáková, E. - Jarošíková, T. - Chernyavskiy, Oleksandr - Hejda, J. - Handl, M. - Beznoska, Jiří - Nečas, A. - Rosina, J. - Amler, Evžen . Polycaprolactone foam functionalized with chitosan microparticles – a suitable scaffold for cartilage regeneration. Physiological Research. 2016, roč. 65, 1, p. 121-131 . IF = 1.461 [ASEP]
Wilhelm, Jiří - Vytášek, Richard - Uhlík, Jiří - Vajner, Luděk . Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species . Oxidative Medicine and Cellular Longevity. 2016, roč. 2016, 2016, p. 5057610 . IF = 4.593 [ASEP] [ doi ]
Manakov, D. - Ujčíková, Hana - Pravenec, Michal - Novotný, J. Alterations in the cardiac proteome of the spontaneously hypertensive rat induced by transgenic expression of CD36 . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 177-186 . IF = 3.914 [ASEP] [ doi ]
Ujčíková, Hana - Vošahlíková, Miroslava - Roubalová, Lenka - Svoboda, Petr . Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 11-23 . IF = 3.914 [ASEP] [ doi ]
Brejchová, Jana - Vošahlíková, Miroslava - Roubalová, Lenka - Parenti, M. - Mauri, M. - Chernyavskiy, Oleksandr - Svoboda, Petr . Plasma membrane cholesterol level and agonist-induced internalization of delta-opioid receptors; colocalization study with intracellular membrane markers of Rab family\\n . Journal of Bioenergetics and Biomembranes. 2016, roč. 48, 4, p. 375-396 . IF = 2.576 [ASEP] [ doi ]
Techniková, L. - Tunák, M. - Janáček, Jiří . Pilling evaluation of patterned fabrics based on a gradient field method . Indian Journal of Fibre & Textile Research. 2016, roč. 41, 1, p. 97-101 . IF = 0.430 [ASEP]
Kolesová, H. - Čapek, Martin - Radochová, Barbora - Janáček, Jiří - Sedmera, David . Comparison of different tissue clearing methods and 3D imaging techniques for visualization of GFP-expressing mouse embryos and embryonic hearts . Histochemistry and Cell Biology. 2016, roč. 146, 2, p. 142-152 . IF = 2.553 [ASEP] [ doi ]
Tonar, Z. - Tomášek, P. - Loskot, P. - Janáček, Jiří - Králíčková, M. - Witter, K. Vasa vasorum in the tunica media and tunica adventitia of the porcine aorta . Annals of Anatomy-Anatomischer Anzeiger. 2016, roč. 205, 2016, p. 22-36 . IF = 1.864 [ASEP] [ doi ]
Vošahlíková, Miroslava - Svoboda, Petr . Lithium - therapeutic tool endowed with multiple beneficiary effects caused by multiple mechanisms . Acta Neurobiologiae Experimentalis. 2016, roč. 76, 1, p. 1-19 . IF = 1.207 [ASEP]
Sládková, J. - Spáčilová, J. - Čapek, Martin - Tesařová, M. - Hansíková, H. - Honzík, T. - Martínek, J. - Zámečník, J. - Kostková, O. - Zeman, J. Analysis of Mitochondrial Network Morphology in Cultured Myoblasts from Patients with Mitochondrial Disorders . Ultrastructural Pathology. 2015, Vol. 39, 5, p. 340-350 . IF = 0.828 [ASEP] [ doi ]
Jirák, D. - Janáček, Jiří - Kear, B. P. A combined MR and CT study for precise quantitative analysis of the avian brain . Scientific Reports. 2015, Vol. 5, Oct 30, p. 16002 . IF = 5.228 [ASEP] [ doi ]
Roubalová, Lenka - Vošahlíková, Miroslava - Brejchová, Jana - Sýkora, Jan - Rudajev, Vladimír - Svoboda, Petr . High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments . PLoS ONE. 2015, Vol. 10, 8, e0135664 . IF = 3.057 [ASEP] [ doi ]
Fernandez, V. - Buffetaut, E. - Suteethorn, V. - Rage, J. C. - Tafforeau, P. - Kundrát, Martin . Evidence of Egg Diversity in Squamate Evolution from Cretaceous Anguimorph Embryos . PLoS ONE. 2015, Vol. 10, 7, e0128610 . IF = 3.057 [ASEP] [ doi ]
Wilhelm, Jiří - Ivica, Josko - Veselská, Zdeňka - Uhlík, Jiří - Vajner, Luděk . Changes in the composition of fatty acids and lipofuscin-like pigments during development of rat heart . Physiological Research. 2015, Vol. 64, 5, p. 643-651 . IF = 1.618 [ASEP]
Michálek, Jan . Total Variation-Based Reduction of Streak Artifacts, Ring Artifacts and Noise in 3D Reconstruction from Optical Projection Tomography . Microscopy and Microanalysis. 2015, Vol. 21, 6, p. 1602-1615 . IF = 1.73 [ASEP] [ doi ]
Kubínová, Lucie - Janáček, Jiří . Confocal stereology: an efficient tool for measurement of microscopic structures . Cell and Tissue Research. 2015, Vol. 360, 1, p. 13-28 . IF = 2.948 [ASEP] [ doi ]
Brejchová, Jana - Sýkora, Jan - Ostašov, Pavel - Merta, Ladislav - Roubalová, Lenka - Janáček, Jiří - Hof, Martin - Svoboda, Petr . TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP . Biochimica Et Biophysica Acta-Biomembranes. 2015, Vol. 1848, 3, p. 781-796 . IF = 3.687 [ASEP] [ doi ]
Cvetko, E. - Čapek, Martin - Damjanovska, M. - Reina, M. A. - Eržen, I. - Stopar-Pintarič, T. The utility of three-dimensional optical projection tomography in nerve injection injury imaging . Anaesthesia. 2015, Vol. 70, 8, p. 939-947 . IF = 3.794 [ASEP] [ doi ]
Bačáková, Markéta - Lopot, F. - Hadraba, Daniel - Varga, Marián - Žaloudková, Margit - Stránská, D. - Suchý, Tomáš - Bačáková, Lucie . Effects of fiber density and plasma modification of nanofibrous membranes on the adhesion and growth of HaCaT keratinocytes . Journal of Biomaterials Applications. 2015, Vol. 29, 6, p. 837-853 . IF = 1.988 [ASEP] [ doi ]
Lišková, Jana - Babchenko, Oleg - Varga, Marián - Kromka, Alexander - Hadraba, Daniel - Švindrych, Zdeněk - Burdíková, Zuzana - Bačáková, Lucie . Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films . International Journal of Nanomedicine. 2015, Vol. 10, 2015, p. 869-884 . IF = 4.32 [ASEP] [ doi ]
Trojanová, Johana - Kulik, A. - Janáček, Jiří - Králíková, Michaela - Syka, Josef - Tureček, Rostislav . Distribution of glycine receptors on the surface of the mature calyx of Held nerve terminal . Frontiers in Neural Circuits 2014, roč. 8, oct, p. 120. ISSN 1662-5110. IF = 3.568 [ASEP] [ doi ]
Líšková, Silvia - Petrová, M. - Karen, Petr - Behuliak, Michal - Zicha, Josef . Contribution of Ca2+-Dependent Cl- Channels to Norepinephrine-Induced Contraction of Femoral Artery Is Replaced by Increasing EDCF Contribution during Ageing . BioMed Research International 2014, roč. 2014, 2014, p. 289361. ISSN 2314-6133. IF = 1.579 [ASEP] [ doi ]
Kubínová, Z. - Janáček, Jiří - Lhotáková, Z. - Kubínová, Lucie - Albrechtová, J. Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach . Journal of Experimental Botany 2014, roč. 65, 2, p. 609-620. ISSN 0022-0957. IF = 5.526 [ASEP] [ doi ]
Janáček, Jiří - Čapek, Martin - Michálek, Jan - Karen, Petr - Kubínová, Lucie . 3D microscopic imaging and evaluation of tubular tissue architecture . Physiological Research 2014, roč. 63, suppl.1, s49-s55. ISSN 0862-8408. IF = 1.293 [ASEP]
Vaněčková, Ivana - Vokurková, Martina - Rauchová, Hana - Dobešová, Zdenka - Pecháňová, O. - Kuneš, Jaroslav - Vorlíček, Jaroslav - Zicha, Josef . Chronic antioxidant therapy lowers blood pressure in adult but not in young Dahl salt hypertensive rats: the role of sympathetic nervous system . Acta Physiologica, 2013, Vol. 208, 4, p. 340-349 . IF = 4.382 [ASEP] [ doi ]
Michálek, Jan - Čapek, Martin . A Piecewise Monotone Subgradient Algorithm for Accurate L1 -TV Based Registration of Physical Slices With Discontinuities in Microscopy . IEEE Transactions on Medical Imaging, 2013, Vol. 32, 5, p. 901-918 . IF = 4.027 [ASEP] [ doi ]
Kubínová, Lucie - Mao, X. W. - Janáček, Jiří . Blood Capillary Length Estimation from Three-Dimensional Microscopic Data by Image Analysis and Stereology . Microscopy and Microanalysis, 2013, Vol. 19, 4, p. 898-906 . IF = 2.495 [ASEP] [ doi ]
Cvetko, E. - Janáček, Jiří - Kubínová, Lucie - Eržen, I. The capillary pattern in human masseter muscle during ageing . Image Analysis and Stereology, 2013, Vol. 32, 3, p. 135-144 . IF = 0.639 [ASEP] [ doi ]
Behuliak, Michal - Pintérová, Mária - Bencze, Michal - Petrová, M. - Líšková, Silvia - Karen, Petr - Kuneš, Jaroslav - Vaněčková, Ivana - Zicha, Josef . Ca2+ sensitization and Ca2+ entry in the control of blood pressure and adrenergic vasoconstriction in conscious Wistar-Kyoto and spontaneously hypertensive rats . Journal of Hypertension, 2013, Vol. 31, 10, p. 2025-2035 . IF = 3.806 [ASEP] [ doi ]
Šepitka, J. - Lukeš, J. - Staněk, L. - Filová, Eva - Burdíková, Zuzana - Řezníček, J. Nanoindentation of intervertebral disc tissues localised by SHG imaging . Computer methods in biomechanics and biomedical engineering, 2012, Vol. 15, Suppl.1, p. 335-336 . IF = 1.393 [ASEP] [ doi ]
Vohník, Martin - Burdíková, Zuzana - Wilkinson, D. M. Testate Amoebae Communities in the Rhizosphere of Rhododendron ponticum (Ericaceae) in an Evergreen Broadleaf Forest in Southern Spain . Acta Protozoologica, 2012, Vol. 51, Sp. iss.3, p. 259-269 . IF = 0.984 [ASEP] [ doi ]
Skalský, I. - Szárszoi, O. - Filová, Elena - Pařízek, Martin - Lytvynets, Andriy - Malušková, J. - Lodererová, A. - Brynda, Eduard - Lisá, Věra - Burdíková, Zuzana - Čapek, Martin - Pirk, J. - Bačáková, Lucie . A perivascular system releasing sirolimus prevented intimal hyperplasia in a rabbit model in a medium-term study . International Journal of Pharmaceutics, 2012, Vol. 427, 2, p. 311-319 . IF = 3.458 [ASEP] [ doi ]
Lhotáková, Z. - Urban, Otmar - Dubánková, M. - Cvikrová, Milena - Tomášková, Ivana - Kubínová, Lucie - Zvára, K. - Marek, Michal V. - Albrechtová, J. The impact of long-term CO2 enrichment on sun and shade needles of Norway spruce (Picea abies): Photosynthetic performance, needle anatomy and phenolics accumulation . Plant Science, 2012, 188-189, JUN, p. 60-70 . IF = 2.922 [ASEP] [ doi ]
Jirkovská, M. - Kučera, T. - Kaláb, J. - Jadrníček, M. - Niedobová, V. - Janáček, Jiří - Kubínová, Lucie - Moravcová, M. - Žižka, Z. - Krejčí, V. The branching pattern of villous capillaries and structural changes of placental terminal villi in type 1 diabetes mellitus . Placenta, 2012, Vol. 33, 5, p. 343-351 . IF = 3.117 [ASEP] [ doi ]
Janáček, Jiří - Kreft, M. - Čebašek, V. - Eržen, I. Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy . Journal of Microscopy, 2012, Vol. 246, 2, p. 107-112 . IF = 1.633 [ASEP] [ doi ]
Hudcovic, Tomáš - Kolínská, Jiřina - Klepetář, Jan - Štěpánková, Renata - Řezanka, Tomáš - Šrůtková, Dagmar - Schwarzer, Martin - Erban, V. - Du, Zhengyu - Wells, J. M. - Hrnčíř, Tomáš - Tlaskalová-Hogenová, Helena - Kozáková, Hana . Protective effect of Clostridium tyrobutyricum in acute dextran sodium sulphate-induced colitis: differential regulation of tumour necrosis factor-α and interleukin-18 in BALB/c and severe combined immunodeficiency mice . Clinical and Experimental Immunology, 2012, Vol. 167, 2, p. 356-365 . IF = 3.409 [ASEP] [ doi ]
Cvetko, E. - Karen, Petr - Eržen, I. Wearing of complete dentures reduces slow fibre and enhances hybrid fibre fraction in masseter muscle . Journal of Oral Rehabilitation, 2012, Vol. 39, 8, p. 608-614 . IF = 2.344 [ASEP] [ doi ]
Cvetko, E. - Karen, Petr - Eržen, I. Myosin heavy chain composition of the human sternocleidomastoid muscle . Annals of Anatomy-Anatomischer Anzeiger, 2012, Vol. 194, 5, p. 467-472 . IF = 1.960 [ASEP] [ doi ]
Cvetko, E. - Karen, Petr - Janáček, Jiří - Kubínová, Lucie - Plasencia, A.L. - Eržen, I. Human Masseter Muscle Fibers From the Elderly Express Less Neonatal Myosin Than Those of Young Adults . Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, 2012, Vol. 295, 8, p. 1364-1372 . IF = 1.343 [ASEP] [ doi ]
Burdíková, Zuzana - Čapek, Martin - Švindrych, Zdeněk - Gryndler, M. - Kubínová, Lucie - Holcová, K. Ecology of Testate Amoebae in the Komořany Ponds in the Vltava Basin . Microbial Ecology, 2012, Vol. 64, 1, p. 117-130 . IF = 3.277 [ASEP] [ doi ]
Váchová, Libuše - Šťovíček, V. - Hlaváček, Otakar - Chernyavskiy, Oleksandr - Štěpánek, L. - Kubínová, Lucie - Palková, Z. Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies . Journal of Cell Biology, 2011, Vol. 194, 5, p. 679-687 . IF = 10.264 [ASEP] [ doi ]
Vohník, Martin - Burdíková, Zuzana - Vyhnal, Aleš - Koukol, O. Interactions Between Testate Amoebae and Saprotrophic Microfungi in a Scots Pine Litter Microcosm . Microbial Ecology, 2011, Vol. 61, 3, p. 660-668 . IF = 2.912 [ASEP] [ doi ]
Skalský, I. - Filová, Elena - Szárszoi, O. - Pařízek, Martin - Lytvynets, Andriy - Malušková, J. - Lodererová, A. - Brynda, Eduard - Lisá, Věra - Burdíková, Zuzana - Čapek, Martin - Pirk, J. - Bačáková, Lucie . A periadventitial sirolimus-releasing mesh decreased intimal hyperplasia in a rabbit model . Physiological Research, 2011, Vol. 60, 3, p. 585-588 . IF = 1.555 [ASEP]
Philimonenko, Anatoly - Janáček, Jiří - Snyers, L. - Almeder, M. - Berger, W. - Schmidt, W. - Schofer, C. - Hozák, Pavel . Chromosomal dynamics of cell cycle regulator gene p21 during transcriptional activation . Journal of Structural Biology, 2011, Vol. 173, 2, p. 382-390 . IF = 3.406 [ASEP] [ doi ]
Michálek, Jan - Čapek, Martin - Kubínová, Lucie . Nonrigid Registration of CLSM Images of Physical Sections with Discontinuous Deformations . Microscopy and Microanalysis, 2011, Vol. 17, 6, p. 923-936 . IF = 3.007 [ASEP] [ doi ]
Michálek, Jan - Čapek, Martin - Kubínová, Lucie . Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy . Microscopy Research Technique, 2011, Vol. 74, 9, p. 831-838 . IF = 1.792 [ASEP] [ doi ]
Líšková, Silvia - Petrová, M. - Karen, Petr - Kuneš, Jaroslav - Zicha, Josef . Effects of aging and hypertension on the participation of endothelium-derived constricting factor (EDCF) in norepinephrine-induced contraction of rat femoral artery . European Journal of Pharmacology, 2011, Vol. 667, 1-3, p. 265-270 . IF = 2.516 [ASEP] [ doi ]
Kozina, V. - Geist, D. - Kubínová, Lucie - Bilic, E. - Karnthaler, H. P. - Waitz, T. - Janáček, Jiří - Chernyavskiy, Oleksandr - Krhen, I. - Ježek, D. Visualization of Reinke’s crystals in normal and cryptorchid testis . Histochemistry and Cell Biology, 2011, Vol. 135, 2, p. 215-228 . IF = 2.588 [ASEP] [ doi ]
Kozina, V. - Banek, L. - Kneževič, N. - Geist, D. - Kubínová, Lucie - Kosovič, M. - Rentenberger, Ch. - Vukasovič, A. - Ježek, D. Reinke´s Crystals in Perivascular and Peritubular Leydig Cells . Croatica Chemica Acta, 2011, Vol. 84, 2, p. 159-167 . IF = 0.763 [ASEP] [ doi ]
Kochová, P. - Cimrman, R. - Janáček, Jiří - Witter, K. - Tonar, Z. How to asses, visualize and compare the anisotropy of linear structures reconstructed from optical sections ? A study based on histopathological quantification of human brain microvessels . Journal of Theoretical Biology, 2011, Vol. 286, -, p. 67-78 . IF = 2.208 [ASEP] [ doi ]
Janáček, Jiří - Cvetko, E. - Kubínová, Lucie - Travnik, L. - Eržen, I. A novel method for evaluation of capillarity in human skeletal muscles from confocal 3D images . Microvascular Research, 2011, Vol. 81, 2, p. 231-238 . IF = 2.828 [ASEP] [ doi ]
Hájková, Zuzana - Bugajev, Viktor - Dráberová, Eduarda - Vinopal, Stanislav - Dráberová, Lubica - Janáček, Jiří - Dráber, Petr - Dráber, Pavel . STIM1-Directed Reorganization of Microtubules in Activated Mast Cells . Journal of Immunology, 2011, Vol. 186, 2, p. 913-923 . IF = 5.788 [ASEP]
Grausová, Ľubica - Kromka, Alexander - Burdíková, Zuzana - Eckhardt, Adam - Rezek, Bohuslav - Vacík, Jiří - Haenen, K. - Lisá, Věra - Bačáková, Lucie . Enhanced Growth and Osteogenic Differentiation of Human Osteoblast-Like Cells on Boron-Doped Nanocrystalline Diamond Thin Films . PLoS ONE, 2011, Vol. 6, 6, e20943 . IF = 4.092 [ASEP] [ doi ]
Eržen, I. - Janáček, Jiří - Kubínová, Lucie . Characterization of the capillary network in skeletal muscles from 3D data . Physiological Research, 2011, Vol. 60, 1, p. 1-13 . IF = 1.555 [ASEP]
Dráberová, Eduarda - Vinopal, Stanislav - Morfini, G. - Liu, P. S. - Sládková, Vladimíra - Sulimenko, Tetyana - Burns, M.R. - Solowska, J. - Kulandaivel, K. - De Chadarévian, J.P. - Legido, A. - Mork, S.J. - Janáček, Jiří - Baas, P. - Dráber, Pavel - Kats . Microtubule-severing ATPase spastin in glioblastoma: increased expression in human glioblastoma cell lines and inverse roles in cell motility and proliferation . Journal of Neuropathology and Experimental Neurology, 2011, Vol. 70, 9, p. 811-826 . IF = 4.258 [ASEP] [ doi ]
Čebašek, V. - Eržen, I. - Vyhnal, Aleš - Janáček, Jiří - Ribarič, S. - Kubínová, Lucie . The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod . Microvascular Research, 2010, Vol. 79, 1, p. 40-46 . IF = 2.390 [ASEP] [ doi ]
Pintérová, Mária - Karen, Petr - Kuneš, Jaroslav - Zicha, Josef . Role of nifedipine-sensitive sympathetic vasoconstriction in maintenance of high blood pressure in spontaneously hypertensive rats: effect of Gi-protein inactivation by pertussis toxin . Journal of Hypertension, 2010, Vol. 28, 5, p. 969-978 . IF = 3.980 [ASEP] [ doi ]
Philimonenko, Vlada - Janáček, Jiří - Harata, M. - Hozák, Pavel . Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus . Histochemistry and Cell Biology, 2010, Vol. 134, 3, p. 243-249 . IF = 4.727 [ASEP] [ doi ]
Michálek, Jan - Čapek, M. - Mao, X. W. - Kubínová, Lucie . Lateral Brightness Correction in Confocal Microscopy Images Using Mathematical Morphology Filters . Microscopy and Microanalysis, 2010, Vol. 16, Suppl.2, p. 758-759 . IF = 2.179 [ASEP] [ doi ]
Michálek, Jan - Čapek, M. - Janáček, Jiří - Kubínová, Lucie . Elastic Alignment of Microscopic Images Using Parallel Processing on CUDA-Supported Graphics Processor Units . Microscopy and Microanalysis, 2010, Vol. 16, Suppl.2, p. 730-731 . IF = 2.179 [ASEP] [ doi ]
Mao, X. W. - Favre, C. J. - Fike, J. R. - Kubínová, Lucie - Anderson, E. - Campbell-Beachler, M. - Jones, T. - Smith, A. - Rightnar, S. - Nelson, G. A. High-LET Radiation-Induced Response of Microvessels in the Hippocampus . Radiation Research, 2010, Vol. 173, 4, p. 486-493 . IF = 2.578 [ASEP] [ doi ]
Kubínová, Lucie - Janáček, Jiří - Eržen, I. - Mao, X. W. Measurement of capillary lenght from 3D images acquired by confocal microscopy using image analysis and stereology . Microscopy and Microanalysis, 2010, Vol. 16, Suppl.2, p. 736-737 . IF = 2.179 [ASEP] [ doi ]
Filová, Eva - Burdíková, Zuzana - Rampichová, Michala - Bianchini, P. - Čapek, Martin - Košťáková, E. - Amler, Evžen - Kubínová, Lucie . Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques . Journal of Biomedical Optics, 2010, Vol. 15, 6, 066011-1-066011-7 . IF = 3.188 [ASEP] [ doi ]
Burdíková, Zuzana - Čapek, Martin - Ostašov, Pavel - Machač, Jiří - Pelc, Radek - Mitchell, E.A.D. - Kubínová, Lucie . Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology . Microscopy and Microanalysis, 2010, Vol. 16, 6, p. 735-746 . IF = 2.179 [ASEP] [ doi ]
Burdíková, Zuzana - Čapek, Martin - Ostašov, Pavel - Mitchell, E.A.D. - Machač, Jiří - Kubínová, Lucie . Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae . Microscopy and Microanalysis, 2010, Vol. 16, Suppl.2, p. 1142-1143 . IF = 2.179 [ASEP] [ doi ]
Čapek, Martin - Brůža, Petr - Janáček, Jiří - Karen, Petr - Kubínová, Lucie - Vagnerová, R. Volume reconstruction of large tissue specimens from serial physical sections using confocal microscopy and correction of cutting deformations by elastic registration . Microscopy Research Technique, 2009, Vol. 72, 2, p. 110-119 . IF = 1.850 [ASEP] [ doi ]
Váchová, Libuše - Chernyavskiy, Oleksandr - Strachotová, Dita - Bianchini, P. - Burdíková, Zuzana - Ferčíková, I. - Kubínová, Lucie - Palková, Z. Architecture of developing multicellular yeast colony: spatio-temporal expression of Ato1p ammonium exporter . Environmental Microbiology, 2009, Vol. 11, 7, p. 1866-1877 . IF = 4.909 [ASEP] [ doi ]
Vohník, Martin - Burdíková, Zuzana - Albrechtová, Jana - Vosátka, Miroslav . Testate Amoebae (Arcellinida & Euglyphida) vs. Ericoid Mycorrhizal and DSE Fungi: A Possible Novel Interaction in The Mycorrhizosphere of Ericaceous Plants? . Microbial Ecology, 2009, Vol. 57, 1, p. 203-214 . IF = 3.251 [ASEP] [ doi ]
Tonar, Z. - Janáček, Jiří - Nedorost, L. - Grill, R. - Báča, V. - Záťura, F. Analysis of microcracks caused by drop shatter testing of porcine kidneys . Annals of Anatomy-Anatomischer Anzeiger, 2009, Vol. 191, 3, p. 294-308 . IF = 0.877 [ASEP] [ doi ]
Radochová, Barbora - Tichá, I. Leaf anatomy during leaf development of photoautotrophically in vitro -grown tobacco plants as affected by growth irradiance . Biologia Plantarum, 2009, Vol. 53, 1, p. 21-27 . IF = 1.656 [ASEP] [ doi ]
Pelc, Radek - Ishii, N. - Ashley, C. C. Laser flash photolysis of diazo-2, a caged calcium chelator: The relationship between the extent and rate of smooth muscle relaxation . Journal of Laser Applications, 2009, Vol. 21, 1, p. 32-38 . IF = 0.652 [ASEP] [ doi ]
Kundrát, M. - Janáček, Jiří - Russell, A. P. Developmental patterns of the crocodilian and avian columella auris: reappraisal of interpretations of the derivation of the dorsal hyoid arch in archosaurian tetrapods . Zoological Journal of the Linnean Society, 2009, Vol. 156, 2, p. 384-410 . IF = 2.031 [ASEP] [ doi ]
Kundrát, M. - Janáček, Jiří - Martin, S. Development of transient head cavities during early organogenesis of the Nile Crocodile (Crocodylus niloticus) . Journal of Morphology, 2009, Vol. 270, 9, p. 1069-1083 . IF = 1.706 [ASEP] [ doi ]
Karen, Petr - Števanec, M. - Smerdu, V. - Cvetko, E. - Kubínová, Lucie - Eržen, I. Software for muscle fibre type classification and analysis . European Journal of Histochemistry, 2009, Vol. 53, 2, p. 87-95 . IF = 0.886 [ASEP]
Johnson, M.D. - He, L. - Herman, D. - Wakimoto, H. - Wallace, C. A. - Zídek, Václav - Mlejnek, Petr - Musilová, Alena - Šimáková, Miroslava - Vorlíček, Jaroslav - Křen, Vladimír - Vyklický, O. - Qi, N.R. - Wang, J. - Seidman, Ch. E. - Seidman, J. - Kurtz, . Dissection of Chromosome 18 Blood Pressure and Salt-Sensitivity Quantitative Trait Loci in the Spontaneously Hypertensive Rat . Hypertension, 2009, Vol. 54, 3, p. 639-645 . IF = 6.614 [ASEP] [ doi ]
Janáček, Jiří - Čebašek, V. - Kubínová, Lucie - Ribarič, S. - Eržen, I. 3D Visualization and Measurement of Capillaries Supplying Metabolically Different Fiber Types in the Rat Extensor Digitorum Longus Muscle During Denervation and Reinnervation . Journal of Histochemistry and Cytochemistry, 2009, Vol. 57, 5, p. 437-447 . IF = 2.372 [ASEP] [ doi ]
Griffiths, P. J. - Isackson, H. - Pelc, Radek - Redwood, C.S. - Funari, S.S. - Watkins, H. - Ashley, C. C. Synchronous In Situ ATPase Activity, Mechanics, and Ca2+ Sensitivity of Human and Porcine Myocardium . Biophysical Journal, 2009, Vol. 97, 9, p. 2503-2512 . IF = 4.390 [ASEP] [ doi ]
Chernyavskiy, Oleksandr - Vannucci, Luca - Bianchini, Paolo - Difato, Francesco - Saieh, M. - Kubínová, Lucie . Imaging of mouse experimental melanoma in vivo and ex vivo by combination of confocal and nonlinear microscopy . Microscopy Research Technique, 2009, Vol. 72, 6, p. 411-423 . IF = 1.850 [ASEP]
Radochová, Barbora - Tichá, I. Excess irradiance causes early symptoms of senescence during leaf expansion in photoautotrophically in vitro grown tobacco plants . Photosynthetica, 2008, Vol. 46, 3, p. 471-475 . IF = 1.000 [ASEP]
Pelc, Radek - Hostounský, Z. - Otaki, T. Correlation between off-axis illumination and apodized phase-contrast: two complementary microscopic phase-imaging modes . Journal of Biomedical Optics, 2008, Vol. 13, 5, , 054067-1-054067-12 . IF = 2.970 [ASEP] [ doi ]
Lhotáková, Zuzana - Albrechtová, Jana - Janáček, Jiří - Kubínová, Lucie . Advantages and pitfalls of using free-hand sections of frozen needles for three-dimensional analysis of mesophyll by stereology and confocal microscopy . Journal of Microscopy, 2008, Vol. 232, 1, p. 56-63 . IF = 1.409 [ASEP]
Kuneš, Jaroslav - Dobešová, Zdenka - Musilová, Alena - Zídek, Václav - Vorlíček, Jaroslav - Pravenec, Michal - Křen, Vladimír - Zicha, Josef . Hemodynamic Characterization of Recombinant Inbred Strains: Twenty Years Later . Hypertension Research, 2008, Vol. 31, 8, p. 1659-1668 . IF = 3.146 [ASEP]
Jirkovská, M. - Janáček, Jiří - Kaláb, Josef - Kubínová, Lucie . Three-dimensional Arrangement of the Capillary Bed and Its Relationship to Microrheology in the Terminal Villi of Normal Term Placenta . Placenta, 2008, Vol. 29, 10, p. 892-897 . IF = 2.775 [ASEP] [ doi ]

Article photogallery

Buňky linie HEK 293 exprimující fúzní protein receptoru TRH a zeleného fluoreskujícího proteinu (GFP) TRH-R-eGFP protein in HEK293 cells
A: Laserový konfokální mikroskop Leica SPE s jednofotonovou excitací. B: Laserový konfokální mikroskop Leica SP2 AOBS MP s jedno- i dvoufotonovou excitací.
The entire photogallery

Contacts

Institute of Physiology CAS
Department of Biomathematics
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tel. +420 241 062 768
 

jiri.janacek@fgu.cas.cz

 

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People

  RNDr. Jiří Janáček, PhD.
Head of department
  RNDr. Lucie Kubínová, CSc.
Deputy head of department
  Ing. Martin Čapek, PhD.
Senior researcher (part-time)
  RNDr. Radek Pelc, DPhil.
Senior researcher
  RNDr. Barbora Radochová, PhD.
Senior researcher
  Ing. Mgr. Daniel Hadraba, Ph.D.
Postdoctoral researcher
  Alexandr Čerňavský, PhD.
Postdoctoral researcher
 

Kevin Jia-Lin Loo, PhD.

Postdoctoral researcher
  MUDr. Petr Karen, CSc.
Senior researcher (part-time)
  Ing. Kateřina Špačková
Project manager
  Alena Dědičová
Technician (part-time)
 

 

Biochemistry of Membrane Receptors
  Doc. RNDr. Petr Svoboda, DrSc.
Group leader
  RNDr. Lenka Roubalová, PhD.
Senior researcher
  RNDr. Hana Ujčíková, PhD.
Senior researcher
  Ing. Miroslava Vošahlíková, PhD.
Senior researcher
  Ing. Jan Teisinger, CSc.
Senior researcher
  Mgr. Jana Brejchová, PhD.
Senior researcher
 

RNDr. Kristýna Boušová, Ph.D.

Postdoctoral researcher
  Ing. Kateřina Stolařová
PhD student
  Mgr. Martina Hloušková
PhD student
  Mgr. Kristina Cechová
PhD student
  Mgr. Michaela Czerneková
PhD student
   
   
   
   
   

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