Vrána, J., Cápal, P., Bednářová, M., Doležel, J.
Applied plant cell biology : 395-430, 2014

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Abstract: Flow cytometry is a powerful technique with numerous applications in biomedical research, including immunology, haematology, oncology and other fields. It has also found important applications in plant science where it accompanied scientists for almost four decades. Without its invaluable outcomes, some areas of plant research would not be in the position where they are now. This chapter focuses on exploitation of this state-of-the-art technology for studying plants at cellular and subcellular level, first providing a general overview and then focusing on nuclei and nuclear DNA content – by far the most frequent and most important application of flow cytometry in plant science. We review applications of the method from the early days to recent advances and discuss its applied aspects.
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IEB authors: Martina Bednářová, Petr Cápal, Jaroslav Doležel, Jan Vrána