Recording of electrophysiological activity from dorsal horn neurons in acute spinal cord slices with patch-clamp technique.
The main research interest of our department is to study mechanisms of pain and to explore new possibilities of pain treatment, especially in chronic states. Our experimental work is concentrated on the modulation of nociceptive information at the spinal cord level that is the relay center between the periphery and the higher brain areas. Our goal is to study these modulatory mechanisms in order to improve therapy for pain conditions, such as allodynia, hyperalgesia, neuropathic and cancer related pain. Our focus is on the role of TRPV1 receptors and inflammatory cytokines. In our research we use mainly electrophysiological, immunohistochemical and behavioral methods. In addition, we finalize another project directed towards the role of thyroid hormones (THs) and omega-3-polyunsaturated fatty acid (n-3 PUFA) in the physiology of cardiac and skeletal muscles. Models of hyper- and hypothyroid status are used to analyze potential influence of n-3 PUFA on the induced pathological changes.
Obituary RNDr. Tomáš Soukup, CSc
Projects
Scientists from the Department of Functional Morphology in collaboration with colleagues from The University of Texas showed that paclitaxel induced pain is mediated by TRPV1 and TLR4 receptors.
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N-acylphosphatidylethanolamine (NAPE), as the precursor of anandamide, an endogenous agonist of TRPV1 and cannabinoid receptors, could play a role in modulation of nociceptive signalization in the spinal cord.
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Achievements
Aktualne.TV - interview, 18.3. 2015
The interview with Dr. Paleček about the research whose aim is to eliminate chronic pain.
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Altogether 70 scientists from 17 foreign countries and 6 from the Czech Republic have attended the meeting as registered participants.
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Publications
Szeiffová Bačová, B. - Vinczenzová, C. - Žurmanová, J. - Kašparová, Dita - Knezl, V. - Egan Beňová, T. - Pavelka, Stanislav - Soukup, Tomáš - Tribulová, N.
Altered thyroid status affects myocardial expression of connexin-43 and susceptibility of rat heart to malignant arrhythmias that can be partially normalized by red palm oil intake
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Histochemistry and Cell Biology. 2017, roč. 147, 1, p. 63-73
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IF = 2.553
[ASEP]
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doi
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Kalynovska, Nataliia - Adámek, Pavel - Paleček, Jiří
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TRPV1 receptors contribute to mediate paclitaxel-induced c-Fos expression in spinal cord dorsal horn neurons
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Physiological Research. 2017, roč. 66, 3, p. 549-532
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IF = 1.461
[ASEP]
Mrózková, Petra - Špicarová, Diana - Paleček, Jiří
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Hypersensitivity Induced by Activation of Spinal Cord PAR2 Receptors Is Partially Mediated by TRPV1 Receptors
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PLoS ONE. 2016, roč. 11, 10, článku e0163991
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IF = 2.806
[ASEP]
[
doi
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Szeiffová Bačová, B. - Egan Beňová, T. - Viczenczová, C. - Soukup, Tomáš - Rauchová, Hana - Pavelka, Stanislav - Knezl, V. - Barančík, M. - Tribulová, N.
Cardiac Connexin-43 and PKC Signaling in Rats With Altered Thyroid Status Without and With Omega-3 Fatty Acids Intake
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Physiological Research. 2016, roč. 65, Suppl.1, S77-S90
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IF = 1.461
[ASEP]
Mrózková, Petra - Paleček, Jiří - Špicarová, Diana
.
The role of protease-activated receptor type 2 in nociceptive signaling and pain
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Physiological Research. 2016, roč. 65, 3, p. 357-367
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IF = 1.461
[ASEP]
Molet, J. - Mauborgne, A. - Diallo, Michael - Armand, V. - Geny, D. - Villanueva, L. - Boucher, Y. - Pohl, M.
Microglial Janus kinase/signal transduction and activator of transcription 3 pathway activity directly impacts astrocyte and spinal neuron characteristics
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Journal of Neurochemistry. 2016, roč. 136, 1, p. 133-147
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IF = 4.083
[ASEP]
[
doi
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Radošinská, J. - Kurahara, L. H. - Hiraishi, K. - Viczenczová, C. - Egan Beňová, T. - Szeiffová Bačová, B. - Dosenko, V. - Navarová, J. - Obšitník, B. - Imanaga, I. - Soukup, Tomáš - Tribulová, N.
Modulation of cardiac connexin-43 by omega-3 fatty acid ethyl-ester supplementation demonstrated in spontaneously diabetic rats
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Physiological Research. 2015, Vol. 64, 6, p. 795-806
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IF = 1.618
[ASEP]
Soukup, Tomáš - Diallo, Michael
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Proportions of myosin heavy chain mRNAs, protein isoforms and fiber types in the slow and fast skeletal muscles are maintained after alterations of thyroid status in rats
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Physiological Research. 2015, Vol. 64, 1, p. 111-118
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IF = 1.618
[ASEP]
Li, Y. - Adámek, Pavel - Zhang, H. - Tatsui, C. E. - Rhines, L. D. - Mrózková, Petra - Li, Q. - Kosturakis, A. K. - Cassidy, R. M. - Harrison, D. S. - Cata, J. P. - Sapire, K. - Zhang, H. - Kennamer-Chapman, R. M. - Jawad, A. B. - Ghetti, A. - Yan, J. - Paleček, Jiří - Dougherty, P. M.
The Cancer Chemotherapeutic Paclitaxel Increases Human and Rodent Sensory Neuron Responses to TRPV1 by Activation of TLR4
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Journal of Neuroscience. 2015, Vol. 35, 39, p. 13487-13500
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IF = 5.924
[ASEP]
[
doi
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Soukup, Tomáš - Smerdu, V.
Effect of altered innervation and thyroid hormones on myosin heavy chain expression and fiber type transitions: a mini-review
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Histochemistry and Cell Biology. 2015, Vol. 143, 2, p. 123-130
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IF = 2.78
[ASEP]
[
doi
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Uchytilová, Eva - Špicarová, Diana - Paleček, Jiří
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Single high-concentration capsaicin application prevents c-Fos expression in spinothalamic and postsynaptic dorsal column neurons after surgical incision
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European Journal of Pain. 2015, Vol. 19, 10, p. 1496-1505
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IF = 2.9
[ASEP]
[
doi
]
Uchytilová, Eva - Špicarová, Diana - Paleček, Jiří
.
TRPV1 antagonist attenuates postoperative hypersensitivity by central and peripheral mechanisms
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Molecular Pain 2014, roč. 10, 2014, p. 67. ISSN 1744-8069.
IF = 3.654
[ASEP]
[
doi
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Špicarová, Diana - Nerandžič, Vladimír - Paleček, Jiří
.
Update on the role of spinal cord TRPV1 receptors in pain modulation
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Physiological Research 2014, roč. 63, suppl.1, s225-s236. ISSN 0862-8408.
IF = 1.293
[ASEP]
Špicarová, Diana - Adámek, Pavel - Kalynovska, Nataliia - Mrózková, Petra - Paleček, Jiří
.
TRPV1 receptor inhibition decreases CCL2-induced hyperalgesia
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Neuropharmacology 2014, roč. 81, jun, p. 75-84. ISSN 0028-3908.
IF = 5.106
[ASEP]
[
doi
]
Soukup, Tomáš
.
Effects of long-term thyroid hormone level alterations, n-3 polyunsaturated fatty acid supplementation and statin administration in rats
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Physiological Research 2014, roč. 63, suppl.1, s119-s131. ISSN 0862-8408.
IF = 1.293
[ASEP]
Kopecká, Kateřina - Zachařová, Gisela - Smerdu, V. - Soukup, Tomáš
.
Slow to fast muscle transformation following heterochronous isotransplantation is influenced by host thyroid hormone status
.
Histochemistry and Cell Biology 2014, roč. 142, 6, p. 677-684. ISSN 0948-6143.
IF = 3.054
[ASEP]
[
doi
]
Žurmanová, J. - Soukup, Tomáš
.
Comparison of myosin heavy chain mRNAs, protein isoforms and fiber type proportions in the rat slow and fast muscles
.
Physiological Research, 2013, Vol. 61, 4, p. 445-453
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IF = 1.531
[ASEP]
Rauchová, Hana - Vokurková, Martina - Pavelka, Stanislav - Behuliak, Michal - Tribulová, N. - Soukup, Tomáš
.
N-3 Polyunsaturated Fatty Acids Supplementation Does not Affect Changes of Lipid Metabolism Induced in Rats by Altered Thyroid Status
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Hormone and Metabolic Research, 2013, Vol. 45, 7, p. 507-512
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IF = 2.145
[ASEP]
[
doi
]
Radošinská, J. - Bačová, B. - Knezl, V. - Beňová, T. - Žurmanová, J. - Soukup, Tomáš - Arnoštová, P. - Slezák, J. - Goncalvesová, E. - Tribulová, N.
Dietary omega-3 fatty acids attenuate myocardial arrhythmogenic factors and propensity of the heart to lethal arrhythmias in a rodent model of human essential hypertension
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Journal of Hypertension, 2013, Vol. 31, 9, p. 1876-1885
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IF = 3.806
[ASEP]
[
doi
]
Soukup, Tomáš - Sulimenko, Vadym - Marková, Vladimíra - Kopecká, Kateřina - Zachařová, Gisela - Paleček, Jiří
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Expression of the skeletal calsequestrin isoform in normal and regenerated skeletal muscles and in the hearts of rats with altered thyroid status
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Physiological Research, 2012, Vol. 61, 6, p. 575-586
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IF = 1.531
[ASEP]
Špicarová, Diana - Nerandžič, Vladimír - Paleček, Jiří
.
Modulation of spinal cord synaptic activity by tumor necrosis factor α in a model of peripheral neuropathy
.
Journal of Neuroinflammation, 2011, Vol. 8, -, e177
.
IF = 3.827
[ASEP]
[
doi
]
Říčný, Jan - Soukup, Tomáš
.
Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms
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Physiological Research, 2011, Vol. 60, 6, p. 899-904
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IF = 1.555
[ASEP]
Rauchová, Hana - Mráček, Tomáš - Novák, Petr - Vokurková, Martina - Soukup, Tomáš
.
Glycerol-3-phosphate Dehydrogenase Expression and Oxygen Consumption in Liver Mitochondria of Female and Male Rats with Chronic Alteration of Thyroid Status
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Hormone and Metabolic Research, 2011, Vol. 43, 1, p. 43-47
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IF = 2.188
[ASEP]
[
doi
]
Novák, Petr - Soukup, Tomáš
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Calsequestrin distribution, structure and function, its role in normal and pathological situations and the effect of thyroid hormones
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Physiological Research, 2011, Vol. 60, 3, p. 439-452
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IF = 1.555
[ASEP]
Arnoštová, P. - Jedelsky, P. L. - Soukup, Tomáš - Žurmanová, J.
Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis
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Journal of Biomedicine and Biotechnology, 2011, Vol. 2011, -, e634253
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IF = 2.436
[ASEP]
[
doi
]
Špicarová, Diana - Paleček, Jiří
.
Tumor necrosis factor α sensitizes spinal cord TRPV1 receptors to the endogenous agonist N-oleoyldopamine
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Journal of Neuroinflammation, 2010, Vol. 7, -, p. 49-7
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IF = 5.785
[ASEP]
[
doi
]
Špicarová, Diana - Paleček, Jiří
.
Modulation of AMPA excitatory postsynaptic currents in the spinal cord dorsal horn neurons by insulin
.
Neuroscience, 2010, Vol. 166, 1, p. 305-311
.
IF = 3.215
[ASEP]
[
doi
]
Tribulová, N. - Knezl, V. - Shainberg, A. - Seki, S. - Soukup, Tomáš
.
Thyroid hormones and cardiac arrhythmias
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Vascular Pharmacology, 2010, Vol. 52, 3-4, p. 102-112
.
IF = 2.174
[ASEP]
[
doi
]
Sojka, David - Zachařová, Gisela - Špicarová, Diana - Paleček, Jiří
.
Changes of calcium binding protein expression in spinothalamic tract neurons after peripheral inflammation
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Physiological Research, 2010, Vol. 59, 6, p. 1011-1017
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IF = 1.646
[ASEP]
Novák, Petr - Zachařová, Gisela - Soukup, Tomáš
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Individual, age and sex differences in fiber type composition of slow and fast muscles of adult Lewis rats: comparison with other rat strains
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Physiological Research, 2010, Vol. 59, 5, p. 783-801
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IF = 1.646
[ASEP]
Špicarová, Diana - Paleček, Jiří
.
The Role of The TRPV1 Endogenous Agonist N-Oleoyldopamine in Modulation of Nociceptive Signaling at the Spinal Cord Level
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Journal of Neurophysiology, 2009, Vol. 102, 1, p. 234-243
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IF = 3.483
[ASEP]
[
doi
]
Čížková, D. - Soukup, Tomáš - Mokrý, J.
Nestin Expression Reflects Formation, Revascularization and Reinnervation of New Myofibers in Regenerating Rat Hind Limb Skeletal Muscles
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Cells Tissues Organs, 2009, Vol. 189, 5, p. 338-347
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IF = 3.322
[ASEP]
[
doi
]
Čížková, D. - Soukup, Tomáš - Mokrý, J.
Expression of nestin, desmin and vimentin in intact and regenerating muscle spindles of rat hind limb skeletal muscles
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Histochemistry and Cell Biology, 2009, Vol. 131, 2, p. 197-206
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IF = 3.021
[ASEP]
[
doi
]
Zachařová, Gisela - Paleček, Jiří
.
Parvalbumin and TRPV1 receptor expression in dorsal root ganglion neurons after acute peripheral inflammation
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Physiological Research, 2009, Vol. 58, 2, p. 305-309
.
IF = 1.430
[ASEP]
Zachařová, Gisela - Sojka, David - Paleček, Jiří
.
Changes of parvalbumin expression in the spinal cord after peripheral inflammation
.
Physiological Research, 2009, Vol. 58, 3, p. 435-442
.
IF = 1.430
[ASEP]
Soukup, Tomáš - Smerdu, V. - Zachařová, Gisela
.
Fiber type composition of unoperated rat soleus and extensor digitorum longus muscles after unilateral isotransplantation of a foreign muscle in long-term experiments
.
Physiological Research, 2009, Vol. 58, 2, p. 253-262
.
IF = 1.430
[ASEP]
Mitašíková, M. - Lin, H. - Soukup, Tomáš - Imanaga, I. - Tribulová, N.
Diabetes and thyroid hormones affect connexin-43 and PKC-ε expression in rat heart atria
.
Physiological Research, 2009, Vol. 58, 2, p. 211-217
.
IF = 1.430
[ASEP]
Žurmanová, Jitka - Půta, F. - Stopková, R. - Soukup, Tomáš
.
Real Time RT-PCR with a Newly Designed Set of Promers Confirmed the Presence of 2b and 2x/d Myosim Heavy Chain mRNAs in the Rat Slow Soleus Muscle
.
Physiological Research, 2008, Vol. 57, 6, p. 973-978
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IF = 1.653
[ASEP]
Špicarová, Diana - Paleček, Jiří
.
The role of spinal cord vanilloid (TRPV1) receptors in pain modulation
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Physiological Research, 2008, Vol. 57, Suppl.3, S69-S77
.
IF = 1.653
[ASEP]
Smerdu, V. - Soukup, Tomáš
.
Demonstration of myosim heavy chain isoforms in rat and humans:the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods
.
European Journal of Histochemistry, 2008, Vol. 52, 3, p. 179-189
.
IF = 1.629
[ASEP]
Mysliveček, J. - Klein, M. - Nováková, M. - Říčný, Jan
.
The detection of the non-M2 muscarinic receptor subtype in the rat heart atria and ventricles
.
Naunyn-Schmiedeberg's Archiv of Pharmacology, 2008, Vol. 378, 1, p. 103-116
.
IF = 2.830
[ASEP]
[
doi
]
Knezl, V. - Soukup, Tomáš - Okruhlicová, L. - Slezák, J. - Tribulová, N.
Thyroid hormones modulate occurrence and termination of ventricular fibrillation by both long-term and acute actions
.
Physiological Research, 2008, Vol. 57, Suppl.2, S91-S96
.
IF = 1.653
[ASEP]
Filová, Eva - Jelínek, F. - Handl, M. - Lytvynets, Andriy - Rampichová, Michala - Varga, F. - Činátl, J. - Soukup, Tomáš - Trč, T. - Amler, Evžen
.
Novel composite hyaluronan/type I collagen/fibrin scaffold enhances repair of osteochondral defect in rabbit knee
.
Journal of Biomedical Materials Research. Part B, 2008, Vol. 87, 2, p. 415-424
.
IF = 2.030
[ASEP]
[
doi
]
Drastichová, Zdeňka - Bouřová, Lenka - Lisý, Václav - Hejnová, L. - Rudajev, Vladimír - Stöhr, Jiří - Durchánková, Dana - Ostašov, Pavel - Teisinger, Jan - Soukup, Tomáš - Novotný, Jiří - Svoboda, Petr
.
Subcellular redistribution of trimeric G-proteins – potential mechanism of desensitization of hormone response: internalisation, solubilization, down-regulation
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Physiological Research, 2008, Vol. 57, Suppl.3, S1-S10
.
IF = 1.653
[ASEP]
Asmussen, G. - Punkt, K. - Bartsch, B. - Soukup, Tomáš
.
Specific Metabolic Properties of Rat Oculorotatory Extraocular Muscles Can Be Linked to Their Low Force Requirements
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Investigative Ophthalmology and Visual Science, 2008, Vol. 49, 11, p. 4865-4871
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IF = 3.582
[ASEP]
[
doi
]
Žurmanová, Jitka - Maláčová, Daniela - Půta, F. - Novák, Petr - Říčný, Jan - Soukup, Tomáš
.
Mass spectroscopy analyses of rat 2b myosin heavy chain isoform
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Physiological Research, 2007, Vol. 56, 5, p. 659-662
.
IF = 1.505
[ASEP]
Žurmanová, Jitka - Difato, F. - Maláčová, Daniela - Mejsnar, J. - Štefl, B. - Zahradník, I.
Creatine kinase binds more firmly to the M-band of rabbit skeletal muscle myofibrils in the presence of its substrates
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Molecular and Cellular Biochemistry, 2007, Vol. 305, 1-2, p. 55-61
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IF = 1.707
[ASEP]
Friedrich, C. - Lemm, B. - Soukup, Tomáš - Asmussen, G.
Determination of slow-tonic MyHC immunoreactivity is an important step in the evaluation of muscle spindles in porcine extraocular muscles
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Experimental Eye Research, 2007, Vol. 85, 1, p. 54-64
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IF = 2.651
[ASEP]
Vadászová-Soukup, Adriana - Soukup, Tomáš
.
Dual role of thyroid hormones in rat soleus muscle MyHC isoform expression
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Physiological Research, 2007, Vol. 56, 6, p. 833-836
.
IF = 1.505
[ASEP]