| Abstract |
Fluorescent nanodiamonds are biocompatible nanoparticles with almost unlimited photostable fluorescence. The nanoparticles are widely used in biomedicine and diagnostics and they represent a new platform for the construction of nanosensors and biosensors. Various direct surface modifications have been described recently, however, the surface chemistry and the construction of sophisticated molecular structures on a nanodiamond surface are only currently developing.
The thesis will be focused on the preparation and physical-chemical study of new nanoparticles based on fluorescent nanodiamonds. Mainly, the following topics will be addressed:
- preparation of monodisperse fluorescent nanodiamonds with hydrophobic monolayers and lipid bilayers
- search for the optimum conditions for dynamical modification of these layers with lipophilic ligands
- nanodiamond modification with biocompatible copolymers: poly(hydroxypropyl)methacrylamides and polyoxazolines
- new conjugation processes allowing the attachement of biomolecules on nanodiamonds
- fluorescence study of nanodiamonds modified by new architectures
- analysis of physical-chemical properties of nanodiamonds by quasielastic light scattering, zeta potential, transmission electronic microscopy, gel permeation chromatography and other methods.
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