Intranet Čeština

Analysis of Biologically Important Compounds

Analysis of Biologically Important Compounds Proteins of tooth pulp separated by 2D electrophoresis (excision) and stained with silver.

Main topics:

  • developing of new separation methods (capillary electromigration techniques), for example:
    • affinity electromigration methods
    • application of gold nanoparticles in capillary electromigration methods
    • forensic application (including coupling CE-MS)
  • analysis of connective tissues - proteomic and physiological analysis of biological samples   when many new proteins were identified, for example:
    • proteom of human teeth in connection to decoy
    • insoluble proteins from the avian eggshell, especially cuticle
    • analysis of posttraslational modifications of proteins
    • determination of arising compounds in glycation reaction as well as sites of modifications
    • identification of age-related changes in proteins – oxidation and deamidation of proteins
  • analysis of a broad spectrum of biologically important compounds in cooperation with many departments of our Institute, various Czech Institutes as well as international cooperation (e.g. proteins, steroids, pigments)
  • proteomic facility of Institute of Physiology CAS

 

Projects

Achievements

Publications

Svobodová, Jana - Kofroňová, Olga - Benada, Oldřich - Král, V. - Mikšík, Ivan . Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall . Journal of Chromatography A. 2017, roč. 1517, p. 185-194 . IF = 3.981 [ASEP] [ doi ]
Ošťádal, M. - Lišková, Jana - Hadraba, Daniel - Eckhardt, Adam . Possible Pathogenetic Mechanisms and New Therapeutic Approaches of Pes Equinovarus . Physiological Research. 2017, roč. 66, 3, p. 403-410 . IF = 1.461 [ASEP]
Mikšík, Ivan . Capillary electrochromatography of proteins and peptides (2006–2015) . Journal of Separation Science. 2017, roč. 40, 1, p. 251-271 . IF = 2.557 [ASEP] [ doi ]
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. Proteins and their modifications in a medieval mummy . Protein Science. 2016, roč. 25, 11, p. 2037-2044 . IF = 2.523 [ASEP] [ doi ]
Jágr, Michal - Eckhardt, Adam - Pataridis, Statis - Foltán, R. - Myšák, J. - Mikšík, Ivan . Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 127-136 . IF = 3.914 [ASEP] [ doi ]
Duval, C. - Cassey, P. - Lovell, P.G. - Mikšík, Ivan - Reynolds, S.J. - Spencer, K.A. Maternal influence on eggshell maculation: implications for cryptic camouflaged eggs . Journal of Ornithology. 2016, roč. 157, 1, p. 303-310 . IF = 1.468 [ASEP] [ doi ]
Ošťádal, M. - Eckhardt, Adam - Herget, J. - Mikšík, Ivan - Dungl, P. - Chomiak, J. - Frydrychová, M. - Burian, M. Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus . Molecular and Cellular Biochemistry. 2015, Vol. 401, 1-2, p. 133-139 . IF = 2.613 [ASEP] [ doi ]
Maurer, G. - Portugal, S. J. - Hauber, M. E. - Mikšík, Ivan - Russell, D. G. D. - Cassey, P. First light for avian embryos: eggshell thickness and pigmentation mediate variation in development and UV exposure in wild bird eggs . Functional Ecology. 2015, Vol. 29, 2, p. 209-218 . IF = 5.21 [ASEP] [ doi ]
Ujčíková, Hana - Eckhardt, Adam - Kagan, Dmytro - Roubalová, Lenka - Svoboda, Petr . Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine . Proteome Science 2014, roč. 12, feb 14, p. 11. ISSN 1477-5956. IF = 1.725 [ASEP] [ doi ]
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. - Tagliaro, F. Prince Cangrande’s Collagen: Study of Protein Modification on the Mummy of the Lord of Verona, Italy (1291–1329 AD) . Chromatographia 2014, roč. 77, 21-22, p. 1503-1510. ISSN 0009-5893. IF = 1.411 [ASEP] [ doi ]

Article photogallery

Hewlett-Packard (Agilent) HPLC/MSD 1100 System. Detectors: DAD, fluorescence, mass spectrometer (quadrupole), radioactive and evaporative light-scattering (Polymer Labs.). Agilent LC-MSD Trap XCT-ultra Proteomic software (SpectrumMill). Hewlett-Packard (Agilent) HPLC/MSD 1100 System. Detectors: DAD, fluorescence, mass spectrometer (quadrupole), radioactive and evaporative light-scattering (Polymer Labs.). Agilent LC-MSD Trap XCT-ultra Proteomic software (SpectrumMill).
CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100) CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100)
Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence) Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence)
The entire photogallery

Contacts

Institute of Physiology AS CR, v.v.i.
Department of Analysis of Biologically Important Compounds
Vídeňská 1083
14220 Prague 4
tel. +420 241 062 534
fax. +420 241 062 558
 

 

 
 

People

 

​​doc. Ing. Tomáš Čajka, Ph.D.

head of the department
  Mgr. Adam Eckhardt, Ph.D.
deputy head of the department
  Prof. Ing. Ivan Mikšík, DrSc.
senior researcher
  RNDr. Michal Jágr, Ph.D.
senior researcher
  Ing. Jana Svobodová, Ph.D.
senior researcher
  Mgr. Pavla Sedláková, Ph.D.
senior researcher
  Ing. Statis Pataridis
research assistant
  Mgr. Lucie Kulhavá
PhD student
  Pavla Šmigolová
lab technician

© 2014 INSTITUTE OF PHYSIOLOGY CAS