HomeInstrumentationMS, GC-MS and LC-MSLarge MS Instruments

Large MS Instruments

AB SCIEX TripleTOF™ 5600 with Dionex ULTIMATE 3000 HPLC system

Location:   Department of Mass Spectrometry
Responsible person:   Dr. Josef Cvačka (☎ 220 183 303)

AB SCIEX TripleTOF 5600


The AB SCIEX TripleTOF 5600 is the next generation in Quadrupole TOF technology. Starting with the best-in-class Triple Quad 5500 System, the new AcceleratorTOF Analyzer was developed to add high-resolution at the speed and sensitivity needed to give equivalent CV's and limits of quantitation (LOQ) to high-end triple quadrupoles. The mass spectrometers uniquely integrates comprehensive qualitative exploration, rapid profiling, and high-resolution quantitation workflows on a single platform. It combines the highest sensitivity detection, high-resolution with 100 Hz acquisition speed, and stable ~1 ppm mass accuracy over days of acquisition. The spectrometer is connected with Dionex ULTIMATE 3000 HPLC system. The instrument was installed in summer 2011.


LTQ Orbitrap XL

Location:   Department of Mass Spectrometry
Responsible person:   Dr. Vladimír Vrkoslav (☎ 220 183 461)

LTQ Orbitrap XL


LTQ Orbitrap XL (Thermo Fisher Scientific) is a hybrid FT mass spectrometer combines a linear ion trap MS and the Orbitrap mass analyzer. Ions generated by API (APCI, ESI, nanoESI) are collected in the LTQ XL followed by axial ejection to the C-shaped storage trap which is used to store and collisionally cool ions before injection into the orbitral trap. The ions transferred from the C-Trap are captured in the orbitral trap by rapidly increasing the electric field and the detection of the image current from coherent ion packets takes place after the voltages have stabilized. Signals from each of the orbitral trap outer electrodes are amplified and transformed into a frequency spectrum by fast Fourier transformation which is finally converted into a mass spectrum. Dynamic range up tu 2000 u, excelent mass accuracy (better then 5 ppm), fast cycle times for MS/MS experiments and high sensitivity are features of this instrument. LTQ Orbitrap XL can be coupled to UHPLC system for LC/MS applications. This instrument was installed in January 2008.


Q-Tof micro

Location:   Department of Mass Spectrometry
Responsible person:   Dr. Josef Cvačka (☎ 220 183 303)

Q-Tof micro


Q-Tof micro (Waters) is a quadrupole orthogonal acceleration time-of-flight tandem mass spectrometer that enables exact mass measurement in both MS and MS/MS modes of analysis. ESI, nano-ESI and APCI ion sources are available. Q-Tof micro is coupled to CapLC capillary HPLC for tryptic digest separations. The instrument was installed in 2004.


SYNAPT G2

Location:   Department of Mass Spectrometry
Responsible person:   Dr. Martin Hubálek (☎ 220 183 117)

SYNAPT G2


The Waters SYNAPT G2 is an ion-mobility mass spectrometer which allows to separate gaseous ions not only in terms of mass, but also in terms of their shapes. Q-Tof micro is coupled to Acquity UPLC M-Class and I-Class systems (Waters).

Parameters
instrument type: tandem mass spectrometer with ion-mobility separation
ionization method: electrospray ionization
mass range: from m/z 10 up to m/z 4000
resolution: m/dm = ca. 20000 in standard operation shape - better than ever before allowing scientists to identify sample components that might have remained invisible to them until now.


2× LCQ / LCQ Advantage

Location:   Department of Mass Spectrometry
Electromigration methods
Responsible person:   Dr. Josef Cvačka (☎ 220 183 303)
Dr. Jana Jaklová Dytrtová (☎ 220 183 514)

LCQ Advantage


LCQ (ThermoFisher Scientific) ion-trap mass spectrometers is used for analytical studies. In one instrument, a modified inlet system allows the introduction of various samples in the ionization as well as the reaction regions. LCQ Classic is coupled to capillary electrophoresis.

Parameters
instrument type: mass spectrometer on ion-trap basis
ionization methods: electrospray ionization, atmospheric pressure chemical ionization
mass range: from m/z 50 up to m/z 2000 (extended up to m/z 4000)
resolution: m/dm = ca. 1500 in standard operation




ultrafleXtreme™

Location:   Department of Mass Spectrometry
Responsible person:   Dr. Josef Cvačka (☎ 220 183 303)

Reflex IV


UltrafleXtreme™MALDI-TOF/TOF mass spectrometer (Bruker Daltonics, Germany) is the only instrument combining true 1 kHz smartbeam II laser technology speed in both TOF and TOF/TOF modes with ultra-high performance and extreme flexibility. The instrument was installed in 2011.