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Education |
2009-2013 |
Ph.D. in Biotechnology at Department of abiotic stress, Institute of Molecular and Celullar biology of Plants, Polytechnic University of Valencia, Valencia, Spain.
Project: Regulatory mechanisms of ion and nutrient transporters through arrestin-related trafficking proteins in Saccharomyces cerevisiae. |
2008-2009 |
M.Sc. in Molecular and Cellular Biotechnology at Department of plant development, Institute of Molecular and Celullar biology of Plants,
Polytechnic University of Valencia, Valencia, Spain.
Project: Yeast one-hybrid screening for the identification of proteins involved in gynoecium development in Arabidopsis thaliana. |
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Experience |
since 2017 |
Postdoctoral Fellow at Institute of Organic Chemistry and Biochemistry of the CAS, Department of Protein Biochemistry, Group of Viral and Microbial Proteins. |
2013-2016 |
Marie-Curie fellowship in the ImResFun Initial Training Network
Project: Regulation of intracellular ion homeostasis in Candida interacting with the host.
Department of membrane transport. Institute of Physiology of the CAS, Prague, CR (Supervisor: Hana Sychrová). |
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Expertise |
Biochemistry and Molecular Biology
- PCR, Cloning, site-directed mutagenesis
- Western blot, Northern blot, pull-down, co-inmunoprecipitation
- Protein Engineering, study of protein-protein interactions, FACS
- Quantification of enzymatic activity
Cellular biology
- Cell culture (Saccharomyces cerevisie, Candida spp., macrophages)
- GFP-tagging and subcellular localization of proteins
- Confocal and fluorescence microscopy
Genetics and Microbiology
- Gene deletion, gene tagging, genetic recombination
- Droptests and study of physiological parameters of yeast strains
- Promoter analysis
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