Burešová, V., Kopecký, D., Bartoš, J., Martinek, P., Watanabe, N., Vyhnánek, T., Doležel, J.
THEORETICAL AND APPLIED GENETICS
128:
273-282,
2015
Keywords:
Abstract:
Key message Different blue-aleurone wheats display
major differences in chromosome composition, ranging
from disomic chromosome additions, substitutions, single
chromosome arm introgressions and chromosome
translocation of Thinopyrum ponticum.
Abstract Anthocyanins are of great importance for human
health due to their antioxidant, anti-inflammatory, antimicrobial
and anti-cancerogenic potential. In common
wheat (Triticum aestivum L.) their content is low. However,
elite lines with blue aleurone exhibit significantly increased
levels of anthocyanins. These lines carry introgressed chromatin
from wild relatives of wheat such as Thinopyrum
ponticum and Triticum monococcum. The aim of our study
was to characterize genomic constitutions of wheat lines
with blue aleurone using genomic and fluorescence in situ
hybridization. We used total genomic DNA of Th. ponticum
and two repetitive DNA sequences (GAA repeat and
the Afa family) as probes to identify individual chromosomes.
This enabled precise localization of introgressed Th.
ponticum chromatin. Our results revealed large variation
in chromosome constitutions of the blue-aleurone wheats.
Of 26 analyzed lines, 17 carried an introgression from
Th. ponticum; the remaining nine lines presumably carry
T. monococcum chromatin undetectable by the methods
employed. Of the Th. ponticum introgressions, six different
types were present, ranging from a ditelosomic addition
(cv. Blue Norco) to a disomic substitution (cv. Blue Baart),
substitution of complete (homologous) chromosome arms
(line UC66049) and various translocations of distal parts
of a chromosome arm(s). Different types of introgressions
present support a hypothesis that the introgressions activate
the blue aleurone trait present, but inactivated, in common
wheat germplasm.
Fulltext: contact IEB authors
IEB authors: Jan Bartoš,
Veronika Burešová,
Jaroslav Doležel,
David Kopecký
