Semináře

The seminar will be devoted to presenting the results of a doctoral thesis which deals with nucleation, growth, and structuring of polycrystalline diamond thin films. In the first part, two alternative methods used to initiate diamond nucleation and growth are investigated and compared to the standardly established nucleation methods. Spontaneous nucleation is studied on horizontally and vertically oriented non-treated (i.e. absence of pre-existing diamond seeds) silicon substrates in a linear plasma CVD system using different deposition parameters. Furthermore, polymer-based nucleation is...

Cryopreservation of cells is a complex process with many useful applications in basic biological research, medicine and agriculture. In this work we deepened the current understanding of the cryopreservation process both at physical and biological level. Results include characteristics of selected cryoprotectants in liquid phase, during phase transition and in solid phase, as well as their impact on cryopreserved cells states . Specifically, the level of cell viability, state of cell membrane and condition of cell nucleus (nuclear membrane, chromatin condensation, DNA strand breaks) are...