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Analysis of Biologically Important Compounds

Analysis of Biologically Important Compounds Proteins of tooth pulp separated by 2D electrophoresis (excision) and stained with silver.

Main topics:

  • developing of new separation methods (capillary electromigration techniques), for example:
    • affinity electromigration methods
    • application of gold nanoparticles in capillary electromigration methods
    • forensic application (including coupling CE-MS)
  • analysis of connective tissues - proteomic and physiological analysis of biological samples   when many new proteins were identified, for example:
    • proteom of human teeth in connection to decoy
    • insoluble proteins from the avian eggshell, especially cuticle
    • analysis of posttraslational modifications of proteins
    • determination of arising compounds in glycation reaction as well as sites of modifications
    • identification of age-related changes in proteins – oxidation and deamidation of proteins
  • analysis of a broad spectrum of biologically important compounds in cooperation with many departments of our Institute, various Czech Institutes as well as international cooperation (e.g. proteins, steroids, pigments)
  • proteomic facility of Institute of Physiology CAS

 

Projects

Achievements

Publications

Mikšík, Ivan - Pataridis, Statis - Eckhardt, Adam - Sedmera, David . Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome . Protein Journal 2018, roč. 37, 1, p. 21-37 . IF = 1.133 [ASEP]
Kohutiar, Matej - Eckhardt, Adam - Mikšík, Ivan - Šantorová, J. - Wilhelm, Jiří . Proteomic analysis of peroxynitrite-induced protein nitration in isolated beef heart mitochondria . Physiological Research 2018, roč. 67, 2, p. 239-250 . IF = 1.324 [ASEP]
Peševski, Živorad - Kvasilová, A. - Stopková, T. - Naňka, O. - Drobná Krejčí, Eliška - Buffinton, Ch. - Kočková, Radka - Eckhardt, Adam - Sedmera, David . Endocardial Fibroelastosis is Secondary to Hemodynamic Alterations in the Chick Embryonic Model of Hypoplastic Left Heart Syndrome . Developmental Dynamics 2018, roč. 247, 3, p. 509-520 . IF = 2.507 [ASEP] [ doi ]
Marschner, Karel - Musil, Stanislav - Mikšík, Ivan - Dědina, Jiří . Investigation of hydride generation from arsenosugars - Is it feasible for speciation analysis? . Analytica Chimica Acta 2018, roč. 1008, MAY, p. 8-17 . IF = 5.123 [ASEP] [ doi ]
Svobodová, Jana - Kofroňová, Olga - Benada, Oldřich - Král, V. - Mikšík, Ivan . Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall . Journal of Chromatography A. 2017, roč. 1517, p. 185-194 . IF = 3.716 [ASEP] [ doi ]
Ošťádal, M. - Lišková, Jana - Hadraba, Daniel - Eckhardt, Adam . Possible Pathogenetic Mechanisms and New Therapeutic Approaches of Pes Equinovarus . Physiological Research. 2017, roč. 66, 3, p. 403-410 . IF = 1.324 [ASEP]
Mikšík, Ivan . Capillary electrochromatography of proteins and peptides (2006–2015) . Journal of Separation Science. 2017, roč. 40, 1, p. 251-271 . IF = 2.415 [ASEP] [ doi ]
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. Proteins and their modifications in a medieval mummy . Protein Science. 2016, roč. 25, 11, p. 2037-2044 . IF = 2.523 [ASEP] [ doi ]
Jágr, Michal - Eckhardt, Adam - Pataridis, Statis - Foltán, R. - Myšák, J. - Mikšík, Ivan . Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 127-136 . IF = 3.914 [ASEP] [ doi ]
Duval, C. - Cassey, P. - Lovell, P.G. - Mikšík, Ivan - Reynolds, S.J. - Spencer, K.A. Maternal influence on eggshell maculation: implications for cryptic camouflaged eggs . Journal of Ornithology. 2016, roč. 157, 1, p. 303-310 . IF = 1.468 [ASEP] [ doi ]

Article photogallery

Hewlett-Packard (Agilent) HPLC/MSD 1100 System. Detectors: DAD, fluorescence, mass spectrometer (quadrupole), radioactive and evaporative light-scattering (Polymer Labs.). Agilent LC-MSD Trap XCT-ultra Proteomic software (SpectrumMill). Hewlett-Packard (Agilent) HPLC/MSD 1100 System. Detectors: DAD, fluorescence, mass spectrometer (quadrupole), radioactive and evaporative light-scattering (Polymer Labs.). Agilent LC-MSD Trap XCT-ultra Proteomic software (SpectrumMill).
CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100) CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100)
Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence) Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence)
The entire photogallery

Contacts

Institute of Physiology AS CR, v.v.i.
Department of Analysis of Biologically Important Compounds
Vídeňská 1083
14220 Prague 4
tel. +420 241 062 534
fax. +420 241 062 558
 

 

 
 

People

 

​​doc. Ing. Tomáš Čajka, Ph.D.

head of the department
  Mgr. Adam Eckhardt, Ph.D.
deputy head of the department
  Prof. Ing. Ivan Mikšík, DrSc.
senior researcher
  RNDr. Michal Jágr, Ph.D.
senior researcher
  Ing. Jana Svobodová, Ph.D.
senior researcher
  Mgr. Pavla Sedláková, Ph.D.
senior researcher
  Ing. Statis Pataridis
research assistant
  Mgr. Lucie Kulhavá
PhD student
  Mgr. Armenuhi Kirakosjanová
lab technician

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