Centre of Plant Structural and Functional Genomics | of the Institute of Experimental Botany AS CR

Position:

research project leader

Phone:

+420 585 238 715

Fax:

+420 585 238 704

E-mail:

simkovah

ueb [dot] cas [dot] cz

Address:

Šlechtitelů 31, CZ-783 71, Olomouc - Holice, Czech Republic

Room number:

206

Education

Professional Experience

1997 – 2000 Postdoctoral Fellow, Laboratory of Molecular Cytogentics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic
2001 – present Senior Scientist, Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany, Olomouc, Czech Republic
2006 – present Lecturer, Department of Cell Biology and Genetics, Faculty of Science, Palacký University, Olomouc, Czech Republic

Recent Research Activities

Development of procedure for preparation of high-molecular-weight DNA from flow-sorted chromosomes
Optimization of procedure and construction of chromosome-specific BAC libraries
Development of procedure for multiple-displacement amplification of flow-sorted chromosomes
Map-based cloning of a Russian wheat aphid resistance gene
Construction of physical map and sequencing of wheat chromosome arm 7DS
Genome mapping in nanochannel arrays ("optical mapping")

All User Publications:

Number of publications: 79

A physical map of the 1-gigabase bread wheat chromosome 3B

Paux, E., Sourdille, P., Salse, J., Saintenac, C., Choulet, F., Leroy, P., Korol, A., Michalak, M., Kianian, S., Spielmeyer, W., Lagudah, E., Somers, D., Kilian, A., Alaux, M., Vautrin, S., Berges, H., Eversole, K., Appels, R., Šafář, J., Šimková, H., Doležel, J., Bernard, M., Feuillet, C.
SCIENCE 322, 101-104, 2008 Fulltext: PDF link on intranet only
A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS).

Šimková, H., Šafář, J., Suchánková, P., Kovářová, P., Bartoš, J., Kubaláková, M., Janda, J., Číhalíková, J., Mago, R., Lelley, T., Doležel, J.
BMC GENOMICS 9, 23, 2008 Fulltext: PDF link on intranet only
Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley

Šimková, H., Svensson, J.T., Condamine, P., Hřibová, E., Suchánková, P., Bhat, P.R., Bartoš, J., Šafář, J., Close, T.J., Doležel, J.
BMC GENOMICS 9, , 2008 Fulltext: PDF link on intranet only

Production of microgram amounts of DNA from flow-sorted plant chromosomes

Suchánková, Pavla; Šimková, Hana; Svensson, J.T.; Hřibová, Eva; Condamine, P.; Bhat, P.R.; Bartoš, Jan; Šafář, Jan; Close, T.J.; Doležel, Jaroslav
CHROMOSOME RESEARCH 15 [2], 4-5, 2007
Development of SSR markers for the short arm of rye chromosome 1

Kofler, R.; Stift, G.; Gong, L.; Suchánková, Pavla; Šimková, Hana; Doležel, Jaroslav; Lelley, T.
VORTRÄGE FÜR PFLANZENZÜCHTUNG 71, 279-281, 2007 Fulltext: public PDF link
Cot-based cloning and sequencing of the short arm of wheat chromosome 1B

Šimková, H., Janda, J., Hřibová, E., Šafář, J., Doležel, J.
PLANT, SOIL AND ENVIRONMENT 53 [10], 437-441, 2007 Fulltext: public PDF link
Chromosome analysis and sorting

Doležel, J., Kubaláková,M., Suchánková, P., Kovářová, P., Bartoš, J., Šimková, H.

Molecular cytogenetic mapping as a tool to characterize genetic diversity and induced mutants in banana

Doležel, J., Hřibová, E., Šimková, H., Doleželová, M.
Advanced resources for plant genomics: BAC library specific for the short arm of wheat chromosome 1B

Janda, J., Šafář, J., Kubaláková, M., Bartoš, J., Kovářová, P., Suchánková, P., Pateyron, S., Číhalíková, J., Sourdille, P., Šimková, H., Faivre-Rampant, P., Hřibová, E., Bernard, M., Lukaszewski, A., Doležel, J., Chalhoub, B.
PLANT JOURNAL 47, 977-986, 2006

Flow cytogenetic analysis of the wheat genome

Doležel, Jaroslav; Doleželová, Marie; Suchánková, Pavla; Šafář, Jan; Kovářová, Pavlína; Bartoš, Jan; Číhalíková, Jarmila; Šimková, Hana

Centre of Plant Structural and
Functional Genomics
of the Institute of Experimental Botany AS CR