Recording of electrophysiological activity from dorsal horn neurons in acute spinal cord slices with patch-clamp technique.
The main research interest of our department is to study mechanisms of pain and to explore new possibilities of pain treatment, especially in chronic states. Our experimental work is concentrated on the modulation of nociceptive information at the spinal cord level that is the relay center between the periphery and the higher brain areas. Our goal is to study these modulatory mechanisms in order to improve therapy for pain conditions, such as allodynia, hyperalgesia, neuropathic and cancer related pain. Our focus is on the role of TRPV1 receptors and inflammatory cytokines. In our research we use mainly electrophysiological, immunohistochemical and behavioral methods. In addition, we finalize another project directed towards the role of thyroid hormones (THs) and omega-3-polyunsaturated fatty acid (n-3 PUFA) in the physiology of cardiac and skeletal muscles. Models of hyper- and hypothyroid status are used to analyze potential influence of n-3 PUFA on the induced pathological changes.
Obituary RNDr. Tomáš Soukup, CSc
Projects
Scientists from the Department of Functional Morphology in collaboration with colleagues from The University of Texas showed that paclitaxel induced pain is mediated by TRPV1 and TLR4 receptors.
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N-acylphosphatidylethanolamine (NAPE), as the precursor of anandamide, an endogenous agonist of TRPV1 and cannabinoid receptors, could play a role in modulation of nociceptive signalization in the spinal cord.
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Achievements
Aktualne.TV - interview, 18.3. 2015
The interview with Dr. Paleček about the research whose aim is to eliminate chronic pain.
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Altogether 70 scientists from 17 foreign countries and 6 from the Czech Republic have attended the meeting as registered participants.
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Publications
Rauchová, Hana - Vokurková, Martina - Pavelka, Stanislav - Vaněčková, Ivana - Tribulová, N. - Soukup, Tomáš
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Red palm oil supplementation does not increase blood glucose or serum lipids levels in Wistar rats with different thyroid status
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Physiological Research 2018, roč. 67, 2, p. 307-315
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IF = 1.324
[ASEP]
Torres-Pérez, J. V. - Adámek, Pavel - Paleček, Jiří - Vizcaychipi, M. - Nagy, I. - Varga, A.
The NA(v)1.7 blocker protoxin II reduces burn injury-induced spinal nociceptive processing
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Journal of Molecular Medicine-Jmm 2018, roč. 96, 1, p. 75-84
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IF = 4.938
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Nerandžič, Vladimír - Mrózková, Petra - Adámek, Pavel - Špicarová, Diana - Nagy, I. - Paleček, Jiří
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Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine
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British Journal of Pharmacology 2018, roč. 175, 12, p. 2322-2356
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IF = 6.810
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doi
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Szeiffová Bačová, B. - Vinczenzová, C. - Žurmanová, J. - Kašparová, Dita - Knezl, V. - Egan Beňová, T. - Pavelka, Stanislav - Soukup, Tomáš - Tribulová, N.
Altered thyroid status affects myocardial expression of connexin-43 and susceptibility of rat heart to malignant arrhythmias that can be partially normalized by red palm oil intake
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Histochemistry and Cell Biology. 2017, roč. 147, 1, p. 63-73
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IF = 2.164
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doi
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Kalynovska, Nataliia - Adámek, Pavel - Paleček, Jiří
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TRPV1 receptors contribute to mediate paclitaxel-induced c-Fos expression in spinal cord dorsal horn neurons
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Physiological Research. 2017, roč. 66, 3, p. 549-532
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IF = 1.324
[ASEP]
Mrózková, Petra - Špicarová, Diana - Paleček, Jiří
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Hypersensitivity Induced by Activation of Spinal Cord PAR2 Receptors Is Partially Mediated by TRPV1 Receptors
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PLoS ONE. 2016, roč. 11, 10, článku e0163991
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IF = 2.806
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doi
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Szeiffová Bačová, B. - Egan Beňová, T. - Viczenczová, C. - Soukup, Tomáš - Rauchová, Hana - Pavelka, Stanislav - Knezl, V. - Barančík, M. - Tribulová, N.
Cardiac Connexin-43 and PKC Signaling in Rats With Altered Thyroid Status Without and With Omega-3 Fatty Acids Intake
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Physiological Research. 2016, roč. 65, Suppl.1, S77-S90
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IF = 1.461
[ASEP]
Mrózková, Petra - Paleček, Jiří - Špicarová, Diana
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The role of protease-activated receptor type 2 in nociceptive signaling and pain
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Physiological Research. 2016, roč. 65, 3, p. 357-367
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IF = 1.461
[ASEP]
Molet, J. - Mauborgne, A. - Diallo, Michael - Armand, V. - Geny, D. - Villanueva, L. - Boucher, Y. - Pohl, M.
Microglial Janus kinase/signal transduction and activator of transcription 3 pathway activity directly impacts astrocyte and spinal neuron characteristics
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Journal of Neurochemistry. 2016, roč. 136, 1, p. 133-147
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IF = 4.083
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Radošinská, J. - Kurahara, L. H. - Hiraishi, K. - Viczenczová, C. - Egan Beňová, T. - Szeiffová Bačová, B. - Dosenko, V. - Navarová, J. - Obšitník, B. - Imanaga, I. - Soukup, Tomáš - Tribulová, N.
Modulation of cardiac connexin-43 by omega-3 fatty acid ethyl-ester supplementation demonstrated in spontaneously diabetic rats
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Physiological Research. 2015, Vol. 64, 6, p. 795-806
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IF = 1.618
[ASEP]
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TRPV1 receptors are present on small diameter dorsal root ganglia neurons, that are involved in pain perception. Dorsal root ganglia neurons on histological slide stained for the presence of TRPV1 receptors (red) and parvalbumin (green).
For the study of cardiac and skeletal muscles histochemical and immunocytochemical staining of tissue sections is used. On the picture skeletal muscle sections stained for specific muscle proteins is shown enabling us quantitative analysis of different muscle fiber types (převzato z práce Soukup et al., Physiol Res 61: 575-586, 2012).
An important approach in our research is recording of electrophysiological activity from dorsal horn neurons in acute spinal cord slices with patch-clamp technique. This method allows us recording of synaptic activity in the dorsal horn, where the nociceptive information between the peripheral and central nervous system is transmitted. The left image shows dorsal horn in spinal cord slice with a patch microelectrode at low magnification; on the right one a recorded neuron is visible under higher magnification.
