Intranet Čeština

Neurochemistry

Neurochemistry

We study the physiology, biochemistry and pharmacology of cholinergic neurons at molecular level. In our study we mainly employ cell lines but we also use animal models. Our research is focused mainly on these topics:

 

  • Biochemical physiology and pharmacology of cholinergic neurons. Development and differentiation of cholinergic neurons. Synthesis, storage, and release of acetylcholine. Presynaptic regulation of acetylcholine release.
  • Cholinergic mechanisms in pathogenesis of Alzheimer´s disease. Influence of beta-amyloid on acetylcholine metabolism and muscarinic transmission.
  • Molecular pharmacology of muscarinic receptors. Allosteric modulation of receptor activation. Interaction of muscarinic receptors with G-proteins. Modeling of muscarinic receptor signal transduction.

Projects

Achievements

Book: Muscarinic Receptor: From Structure to Animal Models

Dr. Jan Jakubik Institute of Physiology Academy of Sciences and professor. Jaromir Mysliveček Institute of Physiology 1st Faculty of Medicine were approached to write the current list of methods that are used in research of muscarinic receptor as part of a Neuromethods series published by Springer. More

Publications

Randáková, Alena - Dolejší, Eva - Rudajev, Vladimír - Zimčík, Pavel - Doležal, Vladimír - El-Fakahany, E. E. - Jakubík, Jan . Role of membrane cholesterol in differential sensitivity of muscarinic receptor subtypes to persistently bound xanomeline . Neuropharmacology 2018, roč. 133, May 1, p. 129-144 . IF = 4.249 [ASEP] [ doi ]
Boulos, J. F. - Jakubík, Jan - Boulos, J. M. - Randáková, Alena - Momirov, J. Synthesis of novel and functionally selective non‐competitive muscarinic antagonists as chemical probes . Chemical Biology & Drug Design 2018, roč. 91, 1, p. 93-104 . IF = 2.328 [ASEP] [ doi ]
Randáková, Alena - Rudajev, Vladimír - Doležal, Vladimír - Boulos, J. - Jakubík, Jan . Novel long‐acting antagonists of muscarinic ACh receptors . British Journal of Pharmacology 2018, roč. 175, 10, p. 1731-1743 . IF = 6.810 [ASEP] [ doi ]
Jakubík, Jan - Randáková, Alena - Zimčík, Pavel - El-Fakahany, E. E. - Doležal, Vladimír . Binding of N-methylscopolamine to the extracellular domain of muscarinic acetylcholine receptors . Scientific Reports. 2017, roč. 7, Jan 16, článku 40381 . IF = 4.122 [ASEP] [ doi ]
Lyukmanova, E. N. - Shulepko, M. A. - Shenkarev, Z. O. - Bychkov, M. L. - Paramonov, A. S. - Chugunov, A. O. - Kulbatskii, D. S. - Arvaniti, M. - Dolejší, Eva - Schaer, T. - Arseniev, A. S. - Efremov, R. G. - Thomsen, M. S. - Doležal, Vladimír - Bertrand, D. - Dolgikh, D. A. - Kirpichnikov, M. P. Secreted Isoform of Human Lynx1 (SLURP-2): Spatial Structure and Pharmacology of Interactions with Different Types of Acetylcholine Receptors . Scientific Reports. 2016, roč. 6, Aug 3, p. 30698 . IF = 4.259 [ASEP] [ doi ]
Dolejší, Eva - Liraz, O. - Rudajev, Vladimír - Zimčík, Pavel - Doležal, Vladimír - Michaelson, D. M. Apolipoprotein E4 reduces evoked hippocampal acetylcholine release in adult mice . Journal of Neurochemistry. 2016, roč. 136, 3, p. 503-509 . IF = 4.083 [ASEP] [ doi ]
Koivisto, H. - Leinonen, H. - Puurula, M. - Hafez, H. S. - Barrera Alquicer, Glenda - Stridh, M. L. - Waagepetersen, H. S. - Tiainen, M. - Soininen, P. - Zilberter, Y. - Tanila, H. Chronic Pyruvate Supplementation Increases Exploratory Activity and Brain Energy Reserves in Young and Middle-Aged Mice . Frontiers in Aging Neuroscience. 2016, roč. 8, Mar 16, p. 41 . IF = 4.504 [ASEP] [ doi ]
Roubalová, Lenka - Vošahlíková, Miroslava - Brejchová, Jana - Sýkora, Jan - Rudajev, Vladimír - Svoboda, Petr . High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments . PLoS ONE. 2015, Vol. 10, 8, e0135664 . IF = 3.057 [ASEP] [ doi ]
Randáková, Alena - Dolejší, Eva - Rudajev, Vladimír - Zimčík, Pavel - Doležal, Vladimír - El-Fakahany, E. E. - Jakubík, Jan . Classical and atypical agonists activate M1 muscarinic acetylcholine receptors through common mechanisms . Pharmacological Research. 2015, Vol. 97, Jul 2015, p. 27-39 . IF = 4.816 [ASEP] [ doi ]
Michal, Pavel - El-Fakahany, E. E. - Doležal, Vladimír . Changes in Membrane Cholesterol Differentially Influence Preferential and Non-preferential Signaling of the M1 and M3 Muscarinic Acetylcholine Receptors . Neurochemical Research. 2015, Vol. 40, 10, p. 2068-2070 . IF = 2.472 [ASEP] [ doi ]

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Contacts

Institute of Physiology AS CR, v.v.i.
Department of Neurochemistry

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 620
fax. +420 241 062 488
   

jakubik@biomed.cas.cz

People

  Mgr. Jan Jakubík, Ph.D.
head of the department
  MUDr. Vladimír Doležal, DrSc.
deputy head of the department
  RNDr. Vladimír Rudajev Ph.D.
senior researcher
  MUDr. et Mgr. Helena Janíčková, Ph.D.
postdoc
  Mgr. Alena Randáková, Ph.D.
postdoc
  Mgr. Eva Dolejší, Ph.D.
postdoc (maternity leave)
  Romana Ondřejová
technician
  Dana Ungerová
technician

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