http://www.biomed.cas.cz/d312/index.php?section=home
Our group is focused on structural biology (the relationship between the structure and function of certain groups of proteins), particularly we focus on the proteins which participate in the signal transmission in the cell. Among methods we use are recombinant protein expression, biophysical characterization, study of intermolecular interactions, protein structure and interaction surfaces. All these methods enable us to better understand the details how the activity and function of protein-protein complexes is regulated. Our research is focused mainly on:
- Structural biology of 14-3-3 proteins and their complexes
- Mechanism of regulation of proteinkisases CaMKK1, CaMKK2 and ASK1
- Study of inhibition of protease caspase-2 by 14-3-3 protein
- Study of DNA-binding domain of transcription factor FOXO4
Projects
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Phosducin (Pdc), a highly conserved phosphoprotein, plays an important role in the regulation of G-protein signalling, transcriptional control, and modulation of blood pressure. Pdc is negatively regulated by phosphorylation followed by binding to the 14-3-3 protein.
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Our results show the activation of yeast neutral trehalase Nth1 by 14-3-3 proteins from the structural point of view. Our data could be important for understanding of the activation process of Nth1 as well as of the role of 14-3-3 proteins in the regulation of other enzymes.
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Achievements
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Kopecka M, Kosek D, Kukacka Z, Rezabkova L, Man P, Novak P, Obsil T, Obsilova V.
J Biol Chem. 2014 May 16;289(20):13948-61.
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Kacirova M, Kosek D, Kadek A, Man P, Vecer J, Herman P, Obsilova V and Obsil T
J. Biol. Chem. jbc.M115.636563. First Published on May 13, 2015, doi:10.1074/jbc.M115.636563
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Prof. RNDr. Tomáš Obšil, Ph.D. was awarded by the title of professor at The Faculty of Science, Charles University, majoring in Physical Chemistry. Professors graduation will take place December 18, 2014.
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Publications
Kalábová, Dana - Šmídová, Aneta - Petrvalská, Olivia - Alblová, Miroslava - Košek, Dalibor - Man, Petr - Obšil, Tomáš - Obšilová, Veronika
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Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding
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Biochemical and Biophysical Research Communications. 2017, roč. 493, 2, p. 940-945
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IF = 2.559
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Kacířová, Miroslava - Nováček, J. - Man, Petr - Obšilová, Veronika - Obšil, Tomáš
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Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function
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Biophysical Journal. 2017, roč. 112, 7, p. 1339-1349
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IF = 3.495
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Alblová, Miroslava - Šmídová, Aneta - Dočekal, V. - Veselý, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš
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Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1
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Proceedings of the National Academy of Sciences of the United States of America. 2017, roč. 114, 46, E9811-E9820
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IF = 9.504
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Kirubakaran, P. - Pfeiferová, L. - Boušová, Kristýna - Bednárová, L. - Obšilová, Veronika - Vondrášek, J.
Artificial proteins as allosteric modulators of PDZ3 and SH3 in two-domain constructs: A computational characterization of novel chimeric proteins
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Proteins-Structure, Function and Bioinformatics. 2016, roč. 84, 10, p. 1358-1374
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IF = 2.397
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Petrvalská, Olivia - Košek, Dalibor - Kukačka, Zdeněk - Tošner, Z. - Man, Petr - Večeř, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš
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Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1)
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Journal of Biological Chemistry. 2016, roč. 291, 39, p. 20753-20765
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IF = 4.125
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Jirků, Michaela - Lánský, Zdeněk - Bednárová, L. - Šulc, Miroslav - Monincová, L. - Majer, P. - Vyklický ml., Ladislav - Vondrášek, J. - Teisinger, Jan - Boušová, Kristýna
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The characterization of a novel S100A1 binding site in the N-terminus of TRPM1
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International Journal of Biochemistry and Cell Biology. 2016, roč. 78, Sep 2016, p. 186-193
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IF = 3.505
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Kylarová, Salome - Košek, Dalibor - Petrvalská, Olivia - Pšenáková, Katarína - Man, Petr - Večeř, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš
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Cysteine residues mediate high‐affinity binding of thioredoxin to ASK1
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FEBS Journal. 2016, roč. 283, 20, p. 3821-3838
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IF = 3.902
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Jirků, Michaela - Bumba, Ladislav - Bednárová, Lucie - Kubala, M. - Šulc, Miroslav - Franěk, M. - Vyklický ml., Ladislav - Vondrášek, Jiří - Teisinger, Jan - Boušová, Kristýna
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Characterization of the part of N-terminal PIP2 binding site of the TRPM1 channel
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Biophysical Chemistry. 2015, Vol. 207, Dec, p. 135-142
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IF = 2.363
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Kacířová, Miroslava - Košek, Dalibor - Kádek, Alan - Man, Petr - Večeř, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš
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Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein
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Journal of Biological Chemistry. 2015, Vol. 290, 26, p. 16246-16260
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IF = 4.258
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Boušová, Kristýna - Jirků, Michaela - Bumba, Ladislav - Bednárová, Lucie - Šulc, Miroslav - Franěk, M. - Vyklický ml., Ladislav - Vondrášek, Jiří - Teisinger, Jan
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PIP2 and PIP3 interact with N-terminus region of TRPM4 channel
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Biophysical Chemistry. 2015, Vol. 205, Oct 2015, p. 24-32
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IF = 2.363
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Detailed insight on structure of the complex 14-3-3:Nth1 (PDB 5M4A). Alblova et al. (2017).
A) Identification of key phosphorylation sites responsible for 14-3-3-dependent activation of neutral trehalase Nth1. B) Decrease of H/D kinetics for a peptide in Ca-binding domain which lies on the interface between the two proteins induced by Bmh1. C) SAXS low resolution structure of the Nth1:Bmh1 complex. Veisova et al. (2012), Macakova et al. (2013), Kopecka et al. (2014).
Protein 14-3-3 interacts with N- and C-termini of Gtβγ binding interfaces of phosducinu. Kacirova et al. (2015).
