Centre of Plant Structural and
Functional Genomics
of the Institute of Experimental Botany AS CR

Analysis of DAPI Flurescence

Increased sensitivity may be achieved by using a high numerical aperture objective (e.g., Partec 40x1.25 quartz, glycerine).
If needed, excitation light intensity may be decreased by placing a neutral density filter (e.g., NG5) between the UG1 and TK420. This may be necessary when non-linearity of DNA content distributions is observed.

Analysis of Propidium Iodide Flurescence

The use of a high numeric aperture objective (e.g., Partec 40x1.25 quartz, glycerine) is highly recommended.
If needed, excitation light intensity may be decreased by placing a neutral density filter (e.g., NG5) between the EM520 and TK560. This may be necessary when non-linearity of DNA content distributions is observed.