Terminal deletions in Hordeum chilense induced by gametocidal activity of chromosome 2C from Aegilops cylindrica

Cifuentes Z., Said M., Cabrera A.
CHROMOSOME RESEARCH 13: 146-149, 2005

Keywords: Barley, Terminal deletions, gametocidal genes
Abstract: Terminal deletions in Hordeum chilense chromosomes were induced genetically by the gametocidal activity of an alien chromosome 2C derived from Aegilops cylindrica. Diploid organisms such as H.chilense do not tolerate deletions, so them strategy that we followed allowed their obtention in both wheat and tritordeum genetic background. Terminal deletions were observed in all seven H.chilense chromosomes, except in chromosome 7Hch. Unfortunately, we failed to establish many of deletions because of sterility. Fertile homozygous plants with deficiencies in the short arm of chromosomes 1Hch and 3Hch and in the long arm of chromosomes 4Hch and 6Hch were obtained. Deletions in the long arm of chromosome 4Hch were obtained in tritordeum background. Deficiencies in the short arms of both chromosomes 1Hch and 3Hch and in the long arm of chromosome 6Hch were established, both as addition or substitution lines, in wheat background. All these plants showed variations in morphological traits and all of them were fertile. IEB authors: Mahmoud Said