Biochemistry

Tutor	Prof. Ing. Tomáš Ruml, CSc. & Doc. Ing. Michal Hocek, DSc.
Team	Michal Hocek
Topic	Enzymatic construction of functionalized RNAs and study of their use in siRNA
Faculty	FPBT VŠCHT
Abstract	Polymerase synthesis of base-modified oligoribonucleotides and RNA from base-functionalized nucleoside triphosphates (NTPs) will be studied. The base-modifications will include functional groups of different size and reactivity. Stability of these modified RNAs towards ribonucleases and their potential use in RNA interference will be studied. The project will be performed in collaboration of the laboratories of both supervisors at FPBT VŠCHT and IOCB ASCR. Leading references: 1. Hocek, M.; Fojta, M. "Cross-coupling reactions of nucleoside triphosphates followed by polymerase incorporation. Construction and applications of base-functionalized nucleic acids" Org. Biomol. Chem. 2008, 6, 2233-2241. 2. Carthew, R. W.; Sontheimer, E. J. "Origins and Mechanisms of miRNAs and siRNAs" Cell, 2009, 136, 642-655.