Microbiology

Enzymic reactions in supercritical carbon dioxide combine the advantage of biocatalysis (substrate specifity under mild reaction conditions) and supercritical fluids (high mass-transfer rate, easy separation of reaction products from the solvent, environmental benefits).

Ph.D. student will study the following tasks:
i) isolation and preparation of enzymes from Geotrichum candidum
ii) testing the enzyme activity and stability in supercritical carbon dioxide
iii) investigating the enzyme reaction in a continuous-flow reactor and the conversion of the reactions and specificity of enzymes
iv) understanding the kinetic, thermodynamic, and transport phenomena and their influence on the enzyme-catalyzed reactions.