| Abstract |
DNA bearing reactive functional groups in major groove will be prepared by polymerase incorporations of modified nucleoside triphosphates. Bioorthogonal reactions of these groups will be studied, e.g. cross-linking with proteins or reactions changing the size and bulkiness of the substituents for switching or triggering of interactions of DNA with proteins.
Leading references:
1. Raindlová, V.; Pohl, R.; Šanda, M.; Hocek, M. "Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines" Angew. Chem. Int. Ed. 2010, 49, 1064-1066.
2. Macíčková-Cahová, H.; Hocek, M. " Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases" Nucleic Acids Res. 2009, 37, 7612-7622.
3. Kielkowski, P.; Macíčková-Cahová, H.; Pohl, R.; Hocek, M. "Transient and switchable (triethylsilyl)ethynyl protection of DNA against cleavage by restriction endonucleases" Angew. Chem. Int. Ed. 2011, 50, 8727-8730.
4. Dadová, J.; Orság, P.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Vinylsulfonamide and acrylamide modification of DNA for cross-linking with proteins" Angew. Chem. Int. Ed. 2013, 52, 10515-10518.
|
