Organic Chemistry

Tutor	Prof. Ing. Michal Hocek, CSc. DSc.
Team	Michal Hocek
Topic	Nucleotides bearing reactive groups for immobilization of oligonucleotides or for bioconjugations
Faculty	FCHT / FPBT VŠCHT, PřF UK
Abstract	Novel base-modified nucleoside triphosphates bearing reactive groups will be prepared and their polymerase catalyzed incorporation to oligoribonucleotides and DNA or RNA will be studied. The base-modifications will include reactive functional groups for immobilization of oligonucleotides to solid surfaces and for bioconjugations with other biomolecules. Leading references: 1. Dadová, J.; Orság, P.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Vinylsulfonamide and acrylamide modification of DNA for cross-linking with proteins" Angew. Chem. Int. Ed. 2013, 52, 10515-10518. 2. Olszewska, A.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Chloroacetamide-linked nucleotides and DNA for cross-linking with peptides and proteins" Bioconjug. Chem. 2016, 27, 2089–2094.