People

Name Llopis-Torregrosa Vicent
Position Postdoctoral Fellow
Vicent Llopis-Torregrosa, Ph.D.

Vicent Llopis-Torregrosa, Ph.D.

Postdoctoral Fellow


Viral and Microbial Proteins
Institute of Organic Chemistry and Biochemistry ASCR, v.v.i.
Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic

☎: (+420) 220 183 252

 
Education
2009-2013 Ph.D. in Biotechnology at Department of abiotic stress, Institute of Molecular and Celullar biology of Plants, Polytechnic University of Valencia, Valencia, Spain.
Project: Regulatory mechanisms of ion and nutrient transporters through arrestin-related trafficking proteins in Saccharomyces cerevisiae.
2008-2009 M.Sc. in Molecular and Cellular Biotechnology at Department of plant development, Institute of Molecular and Celullar biology of Plants, Polytechnic University of Valencia, Valencia, Spain.
Project: Yeast one-hybrid screening for the identification of proteins involved in gynoecium development in Arabidopsis thaliana.
 
Experience
since 2017 Postdoctoral Fellow at Institute of Organic Chemistry and Biochemistry of the CAS, Department of Protein Biochemistry, Group of Viral and Microbial Proteins.
2013-2016 Marie-Curie fellowship in the ImResFun Initial Training Network
Project: Regulation of intracellular ion homeostasis in Candida interacting with the host.
Department of membrane transport. Institute of Physiology of the CAS, Prague, CR (Supervisor: Hana Sychrová).
 
Expertise
Biochemistry and Molecular Biology
  • PCR, Cloning, site-directed mutagenesis
  • Western blot, Northern blot, pull-down, co-inmunoprecipitation
  • Protein Engineering, study of protein-protein interactions, FACS
  • Quantification of enzymatic activity

Cellular biology

  • Cell culture (Saccharomyces cerevisie, Candida spp., macrophages)
  • GFP-tagging and subcellular localization of proteins
  • Confocal and fluorescence microscopy

Genetics and Microbiology

  • Gene deletion, gene tagging, genetic recombination
  • Droptests and study of physiological parameters of yeast strains
  • Promoter analysis