Akpinar, B.A., Yuce, M., Lucas, S., Vrána, J., Burešová, V., Doležel, J., Budak, H.
SCIENCE
5:
10763,
2015
Keywords:
Abstract:
Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum,
the progenitor of durum and bread wheat, and maintains a rich allelic diversity among its wild
populations. The lack of adequate genetic and genomic resources, however, restricts its exploitation
in wheat improvement. Here, we report next-generation sequencing of the flow-sorted chromosome
5B of T. dicoccoides to shed light into its genome structure, function and organization by exploring
the repetitive elements, protein-encoding genes and putative microRNA and tRNA coding
sequences. Comparative analyses with its counterparts in modern and wild wheats suggest clues
into the B-genome evolution. Syntenic relationships of chromosome 5B with the model grasses can
facilitate further efforts for fine-mapping of traits of interest. Mapping of 5B sequences onto the
root transcriptomes of two additional T. dicoccoides genotypes, with contrasting drought tolerances,
revealed several thousands of single nucleotide polymorphisms, of which 584 shared polymorphisms
on 228 transcripts were specific to the drought-tolerant genotype. To our knowledge, this study
presents the largest genomics resource currently available for T. dicoccoides, which, we believe, will
encourage the exploitation of its genetic and genomic potential for wheat improvement to meet the
increasing demand to feed the world.
Fulltext: contact IEB authors
IEB authors: Veronika Burešová,
Jaroslav Doležel,
Jan Vrána
