Intranet Čeština

Membrane Transport

Membrane Transport Sodium and potassium transporting protein observed in yeast cells through a fluorescence microscope. Green fluorescence corresponds to the protein localized in the cell plasma membrane; red fluorescence visualizes membranes of vacuoles.

We study the proteins transporting compounds and signals across the cell membranes. These proteins, called transporters, assure the uptake of nutrients into the cells, efflux of waste compounds from cells and communication with the environment.  To study the animal and plant transporters, we mainly use a model eukaryotic microorganism – yeasts. Our research is focused mainly on:

  • Structure, function and regulation of cell transport systems at protein molecule level.
  • Role of transporters in specific cell properties and in diseases.
  • Role of transporters in Candida virulence and pathogenicity.
  • Development of new techniques to estimate cell physiological parameters.

Projects

Cell cation and pH homeostasis

The intracellular concentration of potassium and sodium cations, as well as of protons, is strictly regulated via the activity of a series of membrane proteins that mediate the flux of cations and protons with various transport mechanisms. Incorrect functioning of some transporters results in serious disorders and diseases. We study in detail the roles of individual transporters and the impact of their activity on cell fitness. More

Transporters of pathogenic yeasts as targets for new antifungal drugs

Some of the transporters existing in the membranes of pathogenic Candida species differ by its structure and activity from those of the host organism. These transporters thus may serve as targets for the development of new antifungal drugs that will affect yeast cells but not cells of the host. More

Transporters reflected in specific properties of nonconventional yeasts

Some yeast species survive extreme changes in the environmental pH, temperature or osmotic pressure. We aim to identify and characterize specific transporters whose activity contributes to the ability to survive adverse environmental conditions. Acquired knowledge will help to improve the properties of yeast species used in industrial processes. More

Achievements

The best poster award for Klara Richerova

Klara Richterova is the author of award-winning poster at 6th Czech-Swiss symposium BioTech 2014. The work with name „Heterologous expression of three laccases from different source of origin in yeast Saccharomyces cerevisiae and their use for environmental application.“ was born in collaboration with Institute of Organic Chemistry and Biochemistry AS CR. Congratulation!  More

Publications

Zemančíková, Jana - Kodedová, Marie - Papoušková, Klára - Sychrová, Hana . Four Saccharomyces species differ in their tolerance to various stresses though they have similar basic physiological parameters . Folia Microbiologica 2018, roč. 63, 2, p. 217-227 . IF = 1.311 [ASEP] [ doi ]
Zemančíková, Jana - Dušková, Michala - Elicharová, Hana - Papoušková, Klára - Sychrová, Hana . Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters . FEMS Microbiology Letters 2018, roč. 365, 5, článku fny020 . IF = 1.723 [ASEP] [ doi ]
Kolínská, Jiřina - Zákostelecká, Marie - Zemanová, Zdeňka - Lisá, Věra - Goliáš, Jaroslav - Kozáková, Hana - Dvořák, B. Cellular differentiation of non-transformed intestinal epithelial cells is regulated by Lactobacillus rhamnosus and L. casei strains . Physiological Research 2018, roč. 67, 2, p. 261-273 . IF = 1.324 [ASEP]
Herkommerová, Klára - Zemančíková, Jana - Sychrová, Hana - Antošová, Zuzana . Immobilization in polyvinyl alcohol hydrogel enhances yeast storage stability and reusability of recombinant laccase-producing S-cerevisiae . Biotechnology Letters 2018, roč. 40, 2, p. 405-411 . IF = 1.846 [ASEP] [ doi ]
Herkommerová, Klára - Dostál, J. - Pichová, I. Decolorization and detoxification of textile wastewaters by recombinant Myceliophthora thermophila and Trametes trogii laccases . 3 Biotech 2018, 8(12)), 505 . IF = 1.497 [ASEP] [ doi ]
Dibalová-Čuláková, Hana - Alonso-del-Real, J. - Querol, A. - Sychrová, Hana . Expression of heterologous transporters in Saccharomyces kudriavzevii: A strategy for improving yeast salt tolerance and fermentation performance . International Journal of Food Microbiology 2018, roč. 268, Mar 2, p. 27-34 . IF = 3.451 [ASEP] [ doi ]
Antošová, Zuzana - Herkommerová, Klára - Pichová, I. - Sychrová, Hana . Efficient secretion of three fungal laccases from Saccharomyces cerevisiae and their potential for decolorization of textile industry effluent - A comparative study . Biotechnology Progress 2018, roč. 34, 1, p. 69-80 . IF = 1.947 [ASEP] [ doi ]
Rosas-Santiago, P. - Lagunas-Goméz, D. - Yáñez-Domínguez, C. - Vera-Estrella, R. - Zimmermannová, Olga - Sychrová, Hana - Pantoja, O. Plant and yeast cornichon possess a conserved acidic motif required for correct targeting of plasma membrane cargos . Biochimica Et Biophysica Acta-Molecular Cell Research. 2017, roč. 1864, 10, p. 1809-1818 . IF = 4.651 [ASEP] [ doi ]
Papoušková, Klára - Andršová, Markéta - Sychrová, Hana . Lack of cortical endoplasmic reticulum protein Ist2 alters sodium accumulation in Saccharomyces cerevisiae cells . FEMS Yeast Research. 2017, roč. 17, 2, článku fox011 . IF = 2.609 [ASEP] [ doi ]
Kodedová, Marie - Sychrová, Hana . Synthetic antimicrobial peptides of the halictines family disturb the membrane integrity of Candida cells . Biochimica Et Biophysica Acta-Biomembranes. 2017, roč. 1859, 10, p. 1851-1858 . IF = 3.438 [ASEP] [ doi ]

Article photogallery

Třepačka - inkubátor tekutých kultur Incubator of liquid cultures
V plném pracovním nasazení Busy time
V plném pracovním nasazení Busy time
The entire photogallery

Contacts

Fyziologický ústav AV ČR, v.v.i.
Department of Membrane Transport
Vídeňská 1083
14220 Praha 4
tel. +420 241 062 667
fax. +420 241 062 284
 

sychrova@fgu.cas.cz

People

Hana Sychrová, DrSc.
head of the department
Olga Zimmermannová, Ph.D. (née Kinclová)
deputy head of the department

Radka Bardoňová

assistant
  Marie Kodedová, Ph.D.
postdoc
Klára Papoušková, Ph.D. (née Velková)
postdoc

Michala Dušková, Ph.D. (née Bubnová)
postdoc

Jakub Masaryk

postgradual student

Jan Bieber

pregradual student
Pavla Herynková
lab technician

Denisa Rácová

lab technician

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