Inoculation of barley with biofertilizers under different levels of nitrogen application in the newly reclaimed lands

Shaalan MA., Shalaby EE., El-Sayed AA., Said M.
Nile Valley and Red Sea Regional Program (NVRP) Ninth Annual Coordination Meeting (Cairo) Egypt. 11-15 September 1997 : , 1997

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Abstract: A field experiment was conducted in the 1996/97 growing season; under sprinkler irrigation system in the newly reclaimed lands at El-Bustan area. A randomized complete block design was used with four replications. The plot size was 3×3.5 m = 10.5 m2 including 15 rows of 3.5 m long and 20 cm apart. Ten fertilizer treatments were applied including two biofertilizers. Phosphorous fertilizer was applied in one dose at sowing in the form of calcium super phosphate (15.5% P2O5), while nitrogen fertilizer was applied as a different levels (0, 48, 96, and 144 Kg/ha) in three equal doses at sowing, tillering, and shooting stages. Nitrogen fertilizer was in the form of ammonium sulphate (20.6% N). Azottein is composed of different fixers of the genera Klebsiella, Bacillus, Azotobacter, and Azospirillum. Results showed significant differences in grain yield (p < 0.01) among fertilizer treatments. The application of biofertilizer caused an increase in grain yield, especially in the presence of nitrogen at the third level with the phosphorous fertilizers amounted about (2981 and 2960 Kg/ha ) from the combination of ( 96 Kg N+ 35 Kg P2O5/ha + Microbin or Azottein ) with about 327 and 325% increase respectively compared to the control, with no significant difference compared to the full dose of nitrogen and phosphorous fertilizers (144 Kg N +35 Kg P2O5/ha).This experiment revealed the advantage of the application of both biofertilizers, Microbin and Azottein in the presence of ( 96 Kg N + 35 Kg P2O5/ha ), which could enhance plant growth and grain yield of barley, and save about 33% of the nitrogen fertilizer without any losses in the grain yield.
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IEB authors: Mahmoud Said