Intranet Čeština

Adipose Tissue Biology

Adipose Tissue Biology

Our department is studying physiological regulations of metabolisms and their disturbances in obesity and associated diseases (i.e. Metabolic syndrome). In order to help treat these disorders we explore the influence of drugs, diet and natural substances, namely n-3 polyunsaturated fatty acids of marine origin (omega-3). Our results show the importance of the metabolism of adipose tissue for accumulation of body fat and reveal new possibilities in the prevention and treatment of metabolic disorders linked to obesity by modulating adipose tissue metabolism. We investigate mechanisms that regulate metabolism in health and disease by combining experiments on mice and cell models with clinical studies, and we try to apply new knowledge in clinical medicine. While working with the manufacturers of the tested substances in the Czech Republic and Norway, we investigate the possible use of omega-3 to increase the effect of drugs and other substances in the treatment of selected diseases. Research projects of the department reflect the current grant support and the existence of Research laboratories established during 2015.

The "Energy metabolism laboratory" (PI: J. Kopecký) examines the importance of a "futile" cycle involving the triglyceride hydrolysis and re-esterification of fatty acids in adipocytes of white adipose tissue for resistance to obesity and associated metabolic disorders, namely in the context of calorie restriction, omega-3 administration and cold exposure. The influence of the above manipulations on the formation of lipid mediators, mitochondrial metabolism and proliferation of cells in the adipose tissue is also being characterized. Core members: K. Bardova, P. Janovska, J. Hansikova, S. Hornova.

The "Metabolomics laboratory" (PI: O. Kuda) focuses on the analysis of lipid mediators (eicosanoids, docosanoids) in adipose tissue using the UPLC-MS / MS method, in particular to identify the source of production of various mediators and their effect on metabolism and inflammatory properties of adipose tissue cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids (FAHFA). New metabolomic methods are also being developed. Core members: M. Brezinova, M. Oseeva, M. Rombaldova, D. Salkova.

The "Glucose homeostasis laboratory" (PI: M. Rossmeisl), by using the method of hyperinsulinemic-euglycemic clamps, analyzes the insulin-sensitizing effects of omega-3 depending on the lipid form (i.e. triacylglycerols vs. phospholipids) of their administration, as well as the involvement of the endocannabinoid system in these effects. We examine the changes in the intestine and their involvement in differential effects of various lipid forms of omega-3 on the whole-body metabolism. Translational potential regarding the beneficial effects of omega-3 on metabolism will also be tested using the transgenic mice expressing the human version of the transcription factor PPARalpha. Core members: O. Horakova, V. Kalendova, P. Kroupová, K. Sedova

All the above mentioned laboratories are closely collaborating and are engaged in translational research conducted in collaboration with clinical as well as industrial partners.

 

 

The Department is subdivided into three laboratories with complementary focus, which closely collaborate and are engaged in translational research conducted together with clinical as well as industrial partners.

 

Projects

Anti-inflammatory effects of novel lipokines of fatty acid esters of hydroxy fatty acids (FAHFA) family in obesity

White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered [1]. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5]. They consist of a fatty acid (e.g. palmitic acid, PA) esterified to the hydroxyl group of a hydroxy fatty acid (e.g. hydroxystearic acid, HSA), abbreviated as PAHSA. The position of the branching carbon defines a regioisomer (e.g. 5-PAHSA). There are several regioisomer families derived from palmitic, palmitoleic, stearic, oleic, linoleic, and docosahexaenoic acid with tissue-specific distribution documented so far [1-4, 6, 7]. Adipose tissue represents a major site of FAHFAs synthesis [1, 2], but the biosynthetic enzymes involved are unknown [11]. Serine hydrolase carboxyl ester lipase [8] and threonine hydrolases [9] were identified as FAHFA-metabolizing enzymes. In humans, FAHFAs were detected in the serum, breast milk, meconium, and adipose tissues [1, 2, 10]. Example of PAHSA regioisomers: Network representation of FAHFA families linked according to the hydroxy-backbone and colored according to the esterified fatty acid [11] (click for full image). Our hypothesis is that novel FAHFAs derived  from omega-3 PUFA, with anti-inflammatory  properties, could be found in mice and humans and that they can beneficially affect adipose tissue metabolism in obesity,  especially low-grade  inflammation. We are also interested in FAHFA metabolic pathways, which seem to be as complex as eicosanoid-related pathways. Using experiments in cell cultures, mice and humans we explore  the structures, effects  on WAT inflammation,  WAT glucose tolerance and molecular  mechanisms of signaling  of these new lipokines. Our results present a significant advance in research of the mechanisms connecting inflammation, metabolism, and nutritional lipids. More

The role of intestine in differential metabolic effects of various lipid forms of dietary omega-3 fatty acids

Long-chain fatty acids of n-3 series (omega-3), mainly EPA and DHA of marine origin, may protect against obesity and exert hypolipidemic effects. While the mechanisms of omega-3’s effects on lipid metabolism in the liver are relatively well described, changes in metabolism in the intestine due to omega-3 are not entirely understood. Previous studies showed that metabolic effects are stronger when omega-3 are administered as phospholipids (Rossmeisl et al., PlosOne 2012). The main goal of this project is to identify the nature of differential metabolic effects of omega-3 depending on the lipid form of their administration (triacylglycerols, phospholipids or wax esters), mainly with regard to the involvement of the intestine in these effects.   More

Physiological relevance of white adipose tissue plasticity and its relationship to the development of obesity

Sufficient plasticity of white adipose tissue allows to adjust appropriately various metabolic processes in response to changing environmental conditions. Metabolic plasticity of adipose tissue is linked with changes in the size and content of cells within the tissue. A hypothesis will be verified that adipose tissue plasticity, namely the proliferative potential of adipose tissue cells, represents an important factor counteracting toxic effects of fatty acids that are released during the breakdown of lipid stores. Reduced ability of the organism to activate these processes could contribute to the  development of obesity and its metabolic consequences. More

Effects of omega-3 in type 2 diabetic patients – new mechanisms for targeted therapy

Minor dietary components with proven biological effects play an important role in the complex treatment strategy of obesity-related diseases, however our knowledge regarding the mechanisms of their action is relatively limited. The aim of this project is to identify novel mechanisms behind the beneficial effects of omega-3 in patients with type 2 diabetes mellitus (DM), i.e. the only dietary component with clearly demonstrated beneficial effects on dyslipidemia and cardiovascular mortality and morbidity in the diabetic patients.  More

The role of endocannabinoid system in metabolic effects of marine omega-3 phospholipids

Metabolic disturbances in obesity are associated with dysregulation of the endocannabinoid (EC) system. We have shown in mice fed an obesogenic high-fat diet that metabolic effects of long-chain omega-3 fatty acids were stronger when supplied in the phospholipid form (omega-3 PL) as compared with triacylglycerols (Rossmeisl et al. 2012. PloS One). These effects were associated with a more efficient modulation of major EC molecules in white adipose tissue (WAT). More

Achievements

Publications

Rossmeisl, Martin - Pavlišová, Jana - Janovská, Petra - Kuda, Ondřej - Bardová, Kristina - Hansíková, Jana - Svobodová, Michaela - Oseeva, Marina - Veleba, J. - Kopecký Jr., J. - Žáček, P. - Fišerová, E. - Pelikánová, T. - Kopecký, Jan . Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids 2018, roč. 1863, 7, p. 712-725 . IF = 4.966 [ASEP] [ doi ]
Kuda, Ondřej - Rossmeisl, Martin - Kopecký, Jan . Omega-3 fatty acids and adipose tissue biology . Molecular Aspects of Medicine 2018, 64(Dec), 147-160 . IF = 7.344 [ASEP] [ doi ]
Kuda, Ondřej - Březinová, Marie - Šilhavý, Jan - Landa, Vladimír - Zídek, Václav - Dodia, Ch. - Kreuchwig, F. - Vrbacký, Marek - Balas, L. - Durand, T. - Hübner, N. - Fisher, A. B. - Kopecký, Jan - Pravenec, Michal . Nrf2-Mediated Antioxidant Defense and Peroxiredoxin 6 Are Linked to Biosynthesis of Palmitic Acid Ester of 9-Hydroxystearic Acid . Diabetes 2018, roč. 67, 6, p. 1190-1199 . IF = 7.273 [ASEP] [ doi ]
Kuda, Ondřej . On the Complexity of PAHSA Research . Cell Metabolism 2018, 28(4), 541-542 . IF = 20.565 [ASEP] [ doi ]
Březinová, Marie - Kuda, Ondřej - Hansíková, Jana - Rombaldová, Martina - Balas, L. - Bardová, Kristina - Durand, T. - Rossmeisl, Martin - Černá, M. - Straňák, Z. - Kopecký, Jan . Levels of palmitic acid ester of hydroxystearic acid (PAHSA) are reduced in the breast milk of obese mothers . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids 2018, roč. 1863, 2, p. 126-131 . IF = 4.966 [ASEP] [ doi ]
Trnovská, J. - Šilhavý, Jan - Kuda, Ondřej - Landa, Vladimír - Zídek, Václav - Mlejnek, Petr - Šimáková, Miroslava - Strnad, Hynek - Škop, V. - Oliyarnyk, O. - Kazdová, L. - Haluzík, M. - Pravenec, Michal . Salsalate ameliorates metabolic disturbances by reducing inflammation in spontaneously hypertensive rats expressing human C-reactive protein and by activating brown adipose tissue in nontransgenic controls . PLoS ONE. 2017, roč. 12, 6, článku e0179063 . IF = 2.766 [ASEP] [ doi ]
Rombaldová, Martina - Janovská, Petra - Kopecký, Jan - Kuda, Ondřej . Omega-3 fatty acids promote fatty acid utilization and production of pro-resolving lipid mediators in alternatively activated adipose tissue macrophages . Biochemical and Biophysical Research Communications.. 2017, roč. 490, 3, p. 1080-1085 . IF = 2.559 [ASEP] [ doi ]
Pravenec, Michal - Zídek, Václav - Landa, Vladimír - Mlejnek, Petr - Šilhavý, Jan - Šimáková, Miroslava - Trnovská, J. - Škop, V. - Marková, I. - Malínská, H. - Hüttl, M. - Kazdová, L. - Bardová, Kristina - Tauchmannová, Kateřina - Vrbacký, Marek - Nůsková, Hana - Mráček, Tomáš - Kopecký, Jan - Houštěk, Josef . Mutant Wars2 gene in spontaneously hypertensive rats impairs brown adipose tissue function and predisposes to visceral obesity . Physiological Research.. 2017, roč. 66, 6, p. 917-924 . IF = 1.324 [ASEP]
Kuda, Ondřej . Bioactive metabolites of docosahexaenoic acid . Biochimie. 2017, roč. 136, May, p. 12-20 . IF = 3.188 [ASEP] [ doi ]
Flachs, Pavel - Adamcová, Kateřina - Zouhar, Petr - Marques, C. - Janovská, Petra - Viegas, I. - Jones, J. G. - Bardová, Kristina - Svobodová, Michaela - Hansíková, Jana - Kuda, Ondřej - Rossmeisl, Martin - Liisberg, U. - Borkowska, A. G. - Kristiansen, K. - Madsen, L. - Kopecký, Jan . Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype . International Journal of Obesity. 2017, roč. 41, 3, p. 372-380 . IF = 5.151 [ASEP] [ doi ]
Horáková, Olga - Hansíková, Jana - Bardová, Kristina - Gardlo, Alžběta - Rombaldová, Martina - Kuda, Ondřej - Rossmeisl, Martin - Kopecký, Jan . \\nPlasma Acylcarnitines and Amino Acid Levels As an Early Complex Biomarker of Propensity to High-Fat Diet-Induced Obesity in Mice . PLoS ONE. 2016, roč. 11, 5, e0155776 . IF = 2.806 [ASEP] [ doi ]
Slámová, Kristýna - Papoušek, František - Janovská, Petra - Kopecký, Jan - Kolář, František . Adverse Effects of AMP-Activated Protein Kinase alpha 2-Subunit Deletion and High-Fat Diet on Heart Function and Ischemic Tolerance in Aged Female Mice . Physiological Research. 2016, roč. 65, 1, p. 33-42 . IF = 1.461 [ASEP]
Rohm, M. - Schäfer, M. - Laurent, V. - Üstünel, B. E. - Niopek, K. - Algire, C. - Hautzinger, O. - Sijmonsma, T. P. - Zota, A. - Medrikova, D. - Pellegata, N. S. - Ryden, M. - Kulyte, A. - Dahlman, I. - Arner, P. - Petrovic, N. - Cannon, B. - Amri, E. Z. - Kemp, B. E. - Steinberg, G. R. - Janovská, Petra - Kopecký, Jan - Wolfrun, Ch. - Blüher, M. - Diaz, M. B. - Herzig, S. An AMP-activated protein kinase–stabilizing peptide ameliorates adipose tissue wasting in cancer cachexia in mice . Nature Medicine. 2016, roč. 22, 10, p. 1120-1130 . IF = 29.886 [ASEP] [ doi ]
Liisberg, U. - Fauske, K. R. - Kuda, Ondřej - Fjare, E. - Myrmel, L. S. - Norberg, N. - Froyland, L. - Graff, I. E. - Liaset, B. - Kristiansen, K. - Kopecký, Jan - Madsen, L. Intake of a Western diet containing cod instead of pork alters fatty acid composition in tissue phospholipids and attenuates obesity and hepatic lipid accumulation in mice . Journal of Nutritional Biochemistry. 2016, roč. 33, Jul, p. 119-127 . IF = 4.518 [ASEP] [ doi ]
Hu, XQ. - Cifarelli, V. - Sun, SS. - Kuda, Ondřej - Abumrad, N. A. - Su, X. Major role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment . Journal of Lipid Research. 2016, roč. 57, 4, p. 663-673 . IF = 4.810 [ASEP] [ doi ]
Kuda, Ondřej - Březinová, Marie - Rombaldová, Martina - Slavíková, Barbora - Pošta, Martin - Beier, Petr - Janovská, Petra - Veleba, J. - Kopecký Jr., J. - Kudová, Eva - Pelikánová, T. - Kopecký, Jan . Docosahexaenoic Acid–Derived Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) With Anti-inflammatory Properties . Diabetes. 2016, roč. 65, 9, p. 2580-2590 . IF = 8.684 [ASEP] [ doi ]
Bardová, Kristina - Horáková, Olga - Janovská, Petra - Hansíková, Jana - Kůs, Vladimír - van Schothorst, E. M. - Hoevenaars, F.P.M. - Uil, M. - Hensler, Michal - Keijer, J. - Kopecký, Jan . Early differences in metabolic flexibility between obesity-resistant and obesity-prone mice . Biochimie. 2016, roč. 124, May, p. 163-170 . IF = 3.112 [ASEP] [ doi ]
Pavlišová, Jana - Bardová, Kristina - Staňková, B. - Tvrzická, E. - Kopecký, Jan - Rossmeisl, Martin . Corn oil versus lard: Metabolic effects of omega-3 fatty acids in mice fed obesogenic diets with different fatty acid composition . Biochimie. 2016, roč. 124, May, p. 150-162 . IF = 3.112 [ASEP] [ doi ]
Kuda, Ondřej - Rombaldová, Martina - Janovská, Petra - Flachs, Pavel - Kopecký, Jan . Cell type-specific modulation of lipid mediator's formation in murine adipose tissue by omega-3 fatty acids . Biochemical and Biophysical Research Communications. 2016, roč. 469, 3, p. 731-736 . IF = 2.466 [ASEP] [ doi ]
Duivenvoorde, L. P. M. - van Schothorst, E. M. - Swarts, H. M. - Kuda, Ondřej - Steenbergh, E. - Termeulen, S. - Kopecký, Jan - Keijer, J. A Difference in Fatty Acid Composition of Isocaloric High-Fat Diets Alters Metabolic Flexibility in Male C57BL/6JOlaHsd Mice . PLoS ONE. 2015, Vol. 10, 6, e0128515 . IF = 3.057 [ASEP] [ doi ]
Kopecký, Jan - Zicha, Josef . Recent development of biomedical research at the Institute of Physiology of the Czech Academy of Sciences in Prague . Physiological Research. 2015, Vol. 64, 5, p. 595-609 . IF = 1.618 [ASEP]
Veleba, J. - Kopecký Jr., J. - Janovská, Petra - Kuda, Ondřej - Horáková, Olga - Malínská, H. - Kazdová, L. - Oliyarnyk, O. - Škop, V. - Trnovská, J. - Hájek, M. - Škoch, A. - Flachs, Pavel - Bardová, Kristina - Rossmeisl, Martin - Olza, J. - de Castro, S. G. - Calder, P. C. - Gardlo, Alžběta - Fišerová, E. - Jensen, J. - Bryhn, M. - Kopecký, Jan - Pelikánová, T. Combined intervention with pioglitazone and n-3 fatty acids in metformin-treated type 2 diabetic patients: improvement of lipid metabolism . Nutrition & Metabolism. 2015, Vol. 12, Dec 2, p. 52 . IF = 3.28 [ASEP] [ doi ]
Masoodi, M. - Kuda, Ondřej - Rossmeisl, Martin - Flachs, Pavel - Kopecký, Jan . Lipid signaling in adipose tissue: Connecting inflammation & metabolism . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2015, Vol. 1851, 4, p. 503-518 . IF = 4.779 [ASEP] [ doi ]
Sjövall, P. - Rossmeisl, Martin - Hanrieder, J. - Kuda, Ondřej - Kopecký, Jan - Bryhn, M. Dietary uptake of omega-3 fatty acids in mouse tissue studied by time-of-flight secondary ion mass spectrometry (TOF-SIMS) . Analytical and Bioanalytical Chemistry. 2015, Vol. 407, 17, p. 5101-5111 . IF = 3.125 [ASEP] [ doi ]
Villarroya, J. - Flachs, Pavel - Redondo-Angulo, I. - Giralt, M. - Medříková, Daša - Villarroya, F. - Kopecký, Jan - Planavila, A. Fibroblast Growth Factor-21 and the Beneficial Effects of Long-Chain n-3 Polyunsaturated Fatty Acids . Lipids 2014, roč. 49, 11, p. 1081-1089. ISSN 0024-4201. IF = 1.854 [ASEP] [ doi ]
van Schothorst, E. M. - Bunschoten, A. - Hoevenaars, F.P.M. - van der Stelt, I. - Janovská, Petra - Venema, D. - Kopecký, Jan - Hollman, P. C. H. - Keijer, J. Direct comparison of health effects by dietary polyphenols at equimolar doses in wildtype moderate high-fat fed C57BL/6JOlaHsd mice . Food Research International 2014, roč. 65, part a, p. 95-102. ISSN 0963-9969. IF = 2.818 [ASEP] [ doi ]
Teodoro, J.S. - Zouhar, Petr - Flachs, Pavel - Bardová, Kristina - Janovská, Petra - Gomes, A.P. - Duarte, F.V. - Varela, A.T. - Rolo, A.P. - Palmeira, C.M. - Kopecký, Jan . Enhancement of brown fat thermogenesis using chenodeoxycholic acid in mice . International Journal of Obesity 2014, roč. 38, 8, p. 1027-1034. ISSN 0307-0565. IF = 5.004 [ASEP] [ doi ]
Svobodová, Michaela - Andreadou, I. - Skaltsounis, A.-L. - Kopecký, Jan - Flachs, Pavel . Oleuropein as an inhibitor of peroxisome proliferator-activated receptor gamma . Genes and Nutrition. 2014, roč. 9, 1, p. 376. ISSN 1555-8932. IF = 2.794 [ASEP] [ doi ]
Rossmeisl, Martin - Medříková, Daša - van Schothorst, E. M. - Pavlišová, Jana - Kuda, Ondřej - Hensler, Michal - Bardová, Kristina - Flachs, Pavel - Staňková, B. - Vecka, M. - Tvrzická, E. - Žák, A. - Keijer, J. - Kopecký, Jan . Omega-3 phospholipids from fish suppress hepatic steatosis by integrated inhibition of biosynthetic pathways in dietary obese mice . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids 2014, roč. 1841, 2, p. 267-278. ISSN 1388-1981. IF = 5.162 [ASEP] [ doi ]
Pepino, M. Y. - Kuda, Ondřej - Samovski, D. - Abumrad, N. A. Structure-Function of CD36 and Importance of Fatty Acid Signal Transduction in Fat Metabolism . Annual Review of Nutrition 2014, roč. 34, july, p. 281-303. ISSN 0199-9885. IF = 8.359 [ASEP] [ doi ]
Jílková, Zuzana - Hensler, Michal - Medříková, Daša - Janovská, Petra - Horáková, Olga - Rossmeisl, Martin - Flachs, Pavel - Sell, H. - Eckel, J. - Kopecký, Jan . Adipose tissue-related proteins locally associated with resolution of inflammation in obese mice . International Journal of Obesity 2014, roč. 38, 2, p. 216-223. ISSN 0307-0565. IF = 5.004 [ASEP] [ doi ]
Hondares, E. - Gallego-Escuredo, J. M. - Flachs, Pavel - Frontini, A. - Cereijo, R. - Goday, A. - Perugini, J. - Kopecký, P. - Giralt, M. - Cinti, S. - Kopecký, Jan - Villarroya, F. Fibroblast growth factor-21 is expressed in neonatal and pheochromocytoma-induced adult human brown adipose tissue . Metabolism-Clinical and Experimental 2014, roč. 63, 3, p. 312-317. ISSN 0026-0495. IF = 3.894 [ASEP] [ doi ]
Flachs, Pavel - Rossmeisl, Martin - Kopecký, Jan . The effect of n-3 fatty acids on glucose homeostasis and insulin sensitivity. Physiological Research 2014, roč. 63, suppl.1, s93-s118. ISSN 0862-8408. IF = 1.293 [ASEP]
Kuda, Ondřej - Pietka, T. A. - Demianova, Zuzana - Kudová, Eva - Cvačka, Josef - Kopecký, Jan - Abumrad, N. A. Sulfo-N-succinimidyl Oleate (SSO) Inhibits Fatty Acid Uptake and Signaling for Intracellular Calcium via Binding CD36 Lysine 164 . Journal of Biological Chemistry, 2013, Vol. 288, 22, p. 15547-15555 . IF = 4.651 [ASEP] [ doi ]
Janovská, Petra - Flachs, Pavel - Kazdová, L. - Kopecký, Jan . Anti-obesity effect of n-3 polyunsaturated fatty acids in mice fed high-fat diet is independent of cold-induced thermogenesis . Physiological Research, 2013, Vol. 62, 2, p. 153-161 . IF = 1.531 [ASEP]
Flachs, Pavel - Rossmeisl, Martin - Kuda, Ondřej - Kopecký, Jan . Stimulation of mitochondrial oxidative capacity in white fat independent of UCP1: A key to lean phenotype . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids, 2013, Vol. 1831, 5, p. 986-1003 . IF = 4.134 [ASEP] [ doi ]
Rossmeisl, Martin - Macek Jílková, Zuzana - Kuda, Ondřej - Jeleník, Tomáš - Medříková, Daša - Staňková, B. - Kristinsson, B. - Haraldsson, G.G. - Svensen, H. - Stoknes, I. - Sjövall, P. - Magnusson, Y. - Balvers, M.G.J. - Verhoeckx, K.C.M. - Tvrzická, E. Metabolic Effects of n-3 PUFA as Phospholipids Are Superior to Triglycerides in Mice Fed a High-Fat Diet: Possible Role of Endocannabinoids . PLoS ONE, 2012, Vol. 7, 6, e38834 . IF = 3.730 [ASEP] [ doi ]
Ortega, F.J. - Jílková, Zuzana - Moreno-Navarrete, J.M. - Pavelka, S. - Rodriguez-Hermosa, J.I. - Kopecký, Jan - Fernández-Real, J.M. Type I iodothyronine 5′-deiodinase mRNA and activity is increased in adipose tissue of obese subjects . International Journal of Obesity, 2012, Vol. 36, 2, p. 320-324 . IF = 5.221 [ASEP] [ doi ]
Medříková, Daša - Jílková, Zuzana - Bardová, Kristina - Janovská, Petra - Rossmeisl, Martin - Kopecký, Jan . Sex differences during the course of diet-induced obesity in mice: adipose tissue expandability and glycemic control . International Journal of Obesity, 2012, Vol. 36, 2, p. 262-272 . IF = 5.221 [ASEP] [ doi ]
Klaus, S. - Keipert, S. - Rossmeisl, Martin - Kopecký, Jan . Augmenting energy expenditure by mitochondrial uncoupling: a role of AMP-activated protein kinase . Genes and Nutrition, 2012, Vol. 7, 3, p. 369-386 . IF = 3.329 [ASEP] [ doi ]
Horáková, Olga - Medříková, Daša - van Schothorst, E. M. - Bunschoten, A. - Flachs, Pavel - Kůs, Vladimír - Kuda, Ondřej - Bardová, Kristina - Janovská, Petra - Hensler, Michal - Rossmeisl, Martin - Wang-Sattler, R. - Prehn, C. - Adamski, J. - Illig, T. - . Preservation of Metabolic Flexibility in Skeletal Muscle by a Combined Use of n-3 PUFA and Rosiglitazone in Dietary Obese Mice . PLoS ONE, 2012, Vol. 7, 8, e43764 . IF = 3.730 [ASEP] [ doi ]
Hoevenaars, F.P.M. - van Schothorst, E. M. - Horáková, Olga - Voigt, A. - Rossmeisl, Martin - Pico, C. - Caimari, A. - Kopecký, Jan - Klaus, S. - Keijer, J. BIOCLAIMS standard diet (BIOsd): a reference diet for nutritional physiology . Genes and Nutrition, 2012, Vol. 7, 3, p. 399-404 . IF = 3.329 [ASEP] [ doi ]
Pietka, T. A. - Sulkin, M.S. - Kuda, Ondřej - Wang, W. - Zhou, D. - Yamada, K. A. - Yang, K. - Su, X. - Gross, R. W. - Nerbonne, J. M. - Efimov, I. R. - Abumrad, N. A. CD36 Protein Influences Myocardial Ca2+ Homeostasis and Phospholipid Metabolism CONDUCTION ANOMALIES IN CD36-DEFICIENT MICE DURING FASTING . Journal of Biological Chemistry, 2012, Vol. 287, 46, p. 38901-38912 . IF = 4.651 [ASEP] [ doi ]
Pico, C. - Macek Jílková, Zuzana - Kůs, Vladimír - Palou, A. - Kopecký, Jan . Perinatal programming of body weight control by leptin: putative roles of AMP kinase and muscle thermogenesis . American Journal of Clinical Nutrition, 2011, Vol. 94, 6 suppl., 1830S-1837S . IF = 6.669 [ASEP] [ doi ]
Kůs, Vladimír - Flachs, Pavel - Kuda, Ondřej - Bardová, Kristina - Janovská, Petra - Svobodová, Michaela - Macek Jílková, Zuzana - Rossmeisl, Martin - Wang-Sattler, R. - Yu, Z. - Illig, T. - Kopecký, Jan . Unmasking Differential Effects of Rosiglitazone and Pioglitazone in the Combination Treatment with n-3 Fatty Acids in Mice Fed a High-Fat Diet . PLoS ONE, 2011, Vol. 6, 11, e27126 . IF = 4.092 [ASEP] [ doi ]
Hensler, Michal - Bardová, Kristina - Macek Jílková, Zuzana - Wahli, W. - Meztger, D. - Chambon, P. - Kopecký, Jan - Flachs, Pavel . The inhibition of fat cell proliferation by fatty acids in dietary obese mice . Lipids in Health and Disease, 2011, Vol. 10, -, p. 128 . IF = 2.170 [ASEP] [ doi ]
Flachs, Pavel - Rühl, R. - Hensler, Michal - Janovská, Petra - Zouhar, Petr - Kůs, Vladimír - Macek Jílková, Zuzana - Papp, E. - Kuda, Ondřej - Svobodová, Michaela - Rossmeisl, Martin - Tsenov, Grygoriy - Mohamed-Ali, V. - Kopecký, Jan . Synergistic induction of lipid catabolism and anti-inflammatory lipids in white fat of dietary obese mice in response to calorie restriction and n-3 fatty acids . Diabetologia, 2011, Vol. 54, 10, p. 2626-2638 . IF = 6.814 [ASEP] [ doi ]
Beneš, J. - Kazdová, L. - Drahota, Zdeněk - Houštěk, Josef - Medříková, Daša - Kopecký, Jan - Kovářová, Nikola - Vrbacký, Marek - Sedmera, David - Strnad, Hynek - Kolář, Michal - Petrák, J. - Benada, Oldřich - Škaroupková, P. - Červenka, L. - Melenovsky, . Effect of metformin therapy on cardiac function and survival in a volume-overload model of heart failure in rats . Clinical science, 2011, Vol. 121, 1, p. 29-41 . IF = 4.317 [ASEP] [ doi ]
Macek Jílková, Zuzana - Pavelka, Stanislav - Flachs, Pavel - Hensler, Michal - Kůs, Vladimír - Kopecký, Jan . Modulation of type I iodothyronine 5’-deiodinase activity in white adipose tissue by nutrition: possible involvement of leptin . Physiological Research, 2010, Vol. 59, 4, p. 561-569 . IF = 1.646 [ASEP]
Jeleník, Tomáš - Rossmeisl, Martin - Kuda, Ondřej - Macek Jílková, Zuzana - Medříková, Daša - Kůs, Vladimír - Hensler, Michal - Janovská, Petra - Mikšík, Ivan - Baranowski, M. - Gorski, J. - Hébrard, S. - Jensen, T. E. - Flachs, Pavel - Hawley, S. - Violl . AMP-activated Protein Kinase alfa2 Subunit Is Required for the Preservation of Hepatic Insulin Sensitivity by n-3 Polyunsaturated Fatty Acids . Diabetes, 2010, Vol. 59, 11, p. 2737-2746 . IF = 8.889 [ASEP] [ doi ]
van Schothorst, E. M. - Flachs, Pavel - Franssen-van Hal, N. - Kuda, Ondřej - Bunschoten, A. - Molthoff, J. - Vink, C. - Hooiveld, G. J.E.J. - Kopecký, Jan - Keijer, J. Induction of lipid oxidation by polyunsaturated fatty acids of marine origin in small intestine of mice fed a high-fat diet . B M C Genomics, 2009, Vol. 110, 10, p. 1-11 . IF = 3.759 [ASEP] [ doi ]
Rossmeisl, Martin - Jeleník, Tomáš - Jílková, Zuzana - Slámová, Kristýna - Kůs, Vladimír - Hensler, Michal - Medříková, Daša - Povýšil, C. - Flachs, Pavel - Mohamed-Ali, V. - Bryhn, M. - Berge, K. - Holmeide, A.K. - Kopecký, Jan . Prevention and Reversal of Obesity and Glucose Intolerance in Mice by DHA Derivatives . Obesity, 2009, Vol. 17, 5, p. 1023-1031 . IF = 3.366 [ASEP] [ doi ]
Kuda, Ondřej - Staňková, B. - Tvrzická, E. - Hensler, Michal - Jeleník, Tomáš - Rossmeisl, Martin - Flachs, Pavel - Kopecký, Jan . Prominent role of liver in elevated plasma palmitoleate levels in response to rosiglitazone in mice fed high-fat diet . Journal of Physiology and Pharmacology, 2009, Vol. 60, 4, p. 125-140 . IF = 1.489 [ASEP]
Kuda, Ondřej - Jeleník, Tomáš - Jílková, Zuzana - Flachs, Pavel - Rossmeisl, Martin - Hensler, Michal - Kazdová, L. - Ogston, N. - Baranowski, M. - Gorski, J. - Janovská, Petra - Kůs, Vladimír - Polák, J. - Mohamed-Ali, V. - Burcelin, R. - Cinti, S. - Bry . n-3 Fatty acids and rosiglitazone improve insulin sensitivity through additive stimulatory effects on muscle glycogen synthesis in mice fed a high-fat diet . Diabetologia, 2009, Vol. 52, 5, p. 941-951 . IF = 6.551 [ASEP] [ doi ]
Kopecký, Jan - Rossmeisl, Martin - Flachs, Pavel - Kuda, Ondřej - Brauner, Petr - Jílková, Zuzana - Staňková, B. - Tvrzická, E. - Bryhn, M. n-3 PUFA: bioavailability and modulation of adipose tissue function . Proceedings of the Nutrition Society, 2009, Vol. 68, 4, p. 361-369 . IF = 4.321 [ASEP] [ doi ]
Flachs, Pavel - Rossmeisl, Martin - Bryhn, M. - Kopecký, Jan . Cellular and molecular effects of n–3 polyunsaturated fatty acids on adipose tissue biology and metabolism . Clinical science, 2009, Vol. 116, 1, p. 1-16 . IF = 3.982 [ASEP] [ doi ]
Ogston, N. C. - Karastergiou, K. - Hosseinzadeh-Attar, M. J. - Bhome, R. - Madani, R. - Stables, M. - Gilroy, D. - Flachs, Pavel - Hensler, Michal - Kopecký, Jan - Mohamed-Ali, V. Low-dose acetylsalicylic acid inhibits the secretion of interleukin-6 from white adipose tissue . International Journal of Obesity, 2008, Vol. 32, 12, p. 1807-1815 . IF = 3.640 [ASEP] [ doi ]
Mullerová, D. - Kopecký, Jan - Matějková, D. - Muller, L. - Rosmus, J. - Racek, J. - Šefrna, F. - Opatrná, S. - Kuda, Ondřej - Matejovic, M. Negative association between plasma levels of adiponectin and polychlorinated biphenyl 153 in obese women under non-energy-restrictive regime . International Journal of Obesity, 2008, Vol. 32, 12, p. 1875-1878 . IF = 3.640 [ASEP] [ doi ]
Kůs, Vladimír - Pražák, Tomáš - Brauner, Petr - Hensler, Michal - Kuda, Ondřej - Flachs, Pavel - Janovská, Petra - Medříková, Daša - Rossmeisl, Martin - Jílková, Zuzana - Štefl, B. - Pašťálková, Eva - Drahota, Zdeněk - Houštěk, Josef - Kopecký, Jan . Induction of muscle thermogenesis by high-fat diet in mice: association with obesity-resistance . American Journal of Physiology, 2008, Vol. 295, 2, E356-E367 . IF = 3.855 [ASEP] [ doi ]
Müllerová, D. - Kopecký, Jan . White adipose tissue: storage and effector site for environmental pollutants . Physiological Research, 2007, Vol. 56, 4, p. 375-381 . IF = 1.505 [ASEP]
Flachs, Pavel - Šponarová, Jana - Kopecký, P. - Horváth, Ondřej - Šedivá, A. - Nibbelink, M. - Casteilla, L. - Medříková, Daša - Neckář, Jan - Kolář, František - Kopecký, Jan . Mitochondrial uncoupling protein 2 gene transcript levels are elevated in maturating erythroid cells . FEBS Letters, 2007, Vol. 581, 6, p. 1093-1097 . IF = 3.263 [ASEP]

Article photogallery

Zvířecí model obezity. Animal model of obesity.
Práce ve sterilním boxu. Work in sterile box.
Zvířecí model perinatálního programingu: Nutriční podmínky během časného vývoje ovlivňují zdraví a nemoc v dospělosti. Animal model of perinatal programing. Nutritional conditions during early development influence health and disease in adult animals.
The entire photogallery

Contacts

Institute of Physiology AS CR, v.v.i.
Department of Adipose Tissue Biology
Vídeňská 1083
14220 Prague 4
Czech Republic
tel. +420 241 062 554
fax. +420 241 062 599
 

Martin.Rossmeisl@fgu.cas.cz

People

MUDr. Martin Rossmeisl, Ph.D.
head of the department
MUDr. Jan Kopecký, DrSc.
deputy head of the department
RNDr. Ondřej Kuda, Ph.D.
senior researcher
Mgr. Olga Horáková, Ph.D.
senior researcher
Ing. Petra Janovská, Ph.D.
senior researcher
Mgr. Kristina Bardová, Ph.D.
junior researcher 
RNDr. Petr Zouhar, Ph.D.
junior researcher 
Mgr. Kateřina Adamcová
PhD student
Mgr. Petra Kroupová
PhD student
Mgr. Marie Březinová
PhD student
Mgr. Jiří Funda
PhD student
Mgr. Veronika Kalendová
PhD student
Mgr. Marina Oseeva
PhD student
Mgr. Gabriela Sistilli
PhD student
Ing. Lucie Leňková
PhD student
Bc. Ilariia Irodenko
student
Bc. Blanka Špiláková
student

Mgr. Karolína Seďová
technician
Jana Jahelková
technician
Ing. Adéla Krejcárková, Ph.D.
technician
Soňa Hornová
technician

Daniela Šálková
technician

 

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