Čeština
EnglishHistological study of embryo-like structures initiated from hypocotyls segments of flax (Linum usitatissimum L.)
Salaj, J., Petrovska, B., Obert, B., Pretova, A.
PLANT CELL REPORTS 24: 590-595, 2005
Klíčová slova: Embryo-like structure, Flax, Histology, Hypocotyl segments, Organogenesis
Abstrakt: Cultivation of flax hypocotyl segments on MS medium supplemented with auxin (2,4-d, NAA) and combination of auxin (NAA) and cytokinin (BAP, zeatin) resulted in production of callus on the cut ends of segments and prolonged cultivation in globular structures resembling early stages of somatic embryos. Embryo-like structures protruded on the surface directly from the subepidermal layers of hypocotyl segments. Despite these globular structures closely resembling somatic embryos, histological observations did not reveal their embryogenic character– organogenesis was the predominant developmental morphogenic pathway. Based on our experiments, as well as on critical revision of existing reports on flax somatic embryogenesis, we conclude, that there has not yet been convincing histological proof of somatic embyogenesis from flax hypocotyl segments.
DOI:
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Beáta Petrovská
PLANT CELL REPORTS 24: 590-595, 2005
Klíčová slova: Embryo-like structure, Flax, Histology, Hypocotyl segments, Organogenesis
Abstrakt: Cultivation of flax hypocotyl segments on MS medium supplemented with auxin (2,4-d, NAA) and combination of auxin (NAA) and cytokinin (BAP, zeatin) resulted in production of callus on the cut ends of segments and prolonged cultivation in globular structures resembling early stages of somatic embryos. Embryo-like structures protruded on the surface directly from the subepidermal layers of hypocotyl segments. Despite these globular structures closely resembling somatic embryos, histological observations did not reveal their embryogenic character– organogenesis was the predominant developmental morphogenic pathway. Based on our experiments, as well as on critical revision of existing reports on flax somatic embryogenesis, we conclude, that there has not yet been convincing histological proof of somatic embyogenesis from flax hypocotyl segments.
DOI:
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Beáta Petrovská