Biochemie a molekulární biologie

Cahová Group

NudiX enzymes are class of enzymes capable of cleaving nucleoside diphosphates linked to x (any moiety). Their roles are not fully understood as they can cleave small molecules such as NAD and CoA as well as canonical RNA caps. Recent discovery of NAD and CoA as new RNA caps leads to reassessment of the RNA structure and NudiX enzymes roles. Some DNA viruses also code for their own NudiX enzymes. The task of this thesis is to define the real substrate of the viral NudiX enzymes and understand the role of viral NudiX enzymes in the infection.

References:
Cahová, H. et al. NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs. Nature, 519, 374 (2015).
Liu, S.-W. et al. Poxvirus Decapping Enzymes Enhance Virulence by Preventing the Accumulation of dsRNA and the Induction of Innate Antiviral Responses. Cell Host & Microbe, 17, 320 (2015).
Quintas, A. et al. Characterization of the African Swine Fever Virus Decapping Enzyme during Infection. Journal of Virology, 91, e00990-71 (2017).