| Abstract |
Lipids are almost exclusively synthesized in the endoplasmatic reticulum and they must be transported to their proper physiological location. One option is the vesicular transport, the other is transport by lipid transport proteins such as the Oxysterol-binding protein (OSBP). We aim to reconstitute in vitro the active transport of several selected lipids (PI, PI4P, PS, cholesterol) by cellular machinery and later to characterize how viral proteins hijack this machinery in order to modify cellular membranes for viral replication.
Leading references:
1. Dubankova A, Humpolickova J, Klima M, Boura E. (2017) Negative charge and membrane-tethered viral 3B cooperate to recruit viral RNA dependent RNA polymerase 3Dpol; Scientific Reports
2. Klima M, Chalupska D, Rozycki B,Humpolickova J, Rezabkova L, Silhan J, Baumlova A, Dubankova A, Boura E. (2017) Kobuviral Non-structural 3A Proteins Act as Molecular Harnesses to Hijack the Host ACBD3 Protein; Structure
3. Humpolickova J#, Mejdrova I#, Matousova M, Nencka R,* Boura E.* (2017) Fluorescent Inhibitors as Tools To Characterize Enzymes: Case Study of the Lipid Kinase Phosphatidylinositol 4-Kinase IIIb (PI4KB); Journal of Medicinal Chemistry
4. Klima M , Tóth DJ, Hexnerova R, Baumlova A, Chalupska D , Tykvart J, Rezabkova L, Sengupta N, Man P, Dubankova A, Humpolickova J , Nencka R, Veverka V, Balla T, Boura E. (2016) Structural insights and in vitro reconstitution of membrane targeting and activation of human PI4KB by the ACBD3 protein; Scientific Reports
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