Organic Chemistry

Tutor	Prof. Ing. Michal Hocek, PhD. DSc.
Team	Michal Hocek
Topic	New RNA modifications for regulation of translation
Faculty	FCHT or FPBT VŠCHT, PřF UK
Abstract	New types of modified mRNA bearing diverse substituents at nucleobases will be prepared by polymerase incorporation of modified nucleoside triphosphates and the influence of these non-natural substituents on interactions with proteins as well as on translation will be studies. Leading references: 1. Milisavljevič, N.; Perlíková, P.; Pohl, R.; Hocek, M.: "Enzymatic synthesis of base-modified RNA by T7 RNA polymerase. A systematic study and comparison of 5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside triphosphates as substrates" Org. Biomol. Chem. 2018, 16, 5800-5807.