Úvod Výzkum Shared Equipment

Shared Equipment

For equipment booking use our on-line reservation system (requires registration).

Microscopy facility

	Year	Dept	Location	Use for	Manager	Operator
Bruker Ultima IntraVital	2018	60	DAI	upright multiphoton microscope for in vivo and in vitro imaging	Mgr. Daniel Hadraba	Mgr. David Vondrášek
Leica SP8 AOBS WLL MP confocal	2015	60	DAI 10	Inverted laser scanning and multiphoton microscope	Mgr. Daniel Hadraba	Mgr. David Vondrášek
Leica SP2 AOBS	2007	60	DAI 9	Inverted laser scanning and fluorescence microscope	Mgr. Daniel Hadraba	Mgr. David Vondrášek
Leica SPE confocal	2007	60	DAI 9	Upright laser scanning and fluorescence microscope	Mgr. Daniel Hadraba	Mgr. David Vondrášek
Optical projection tomography	2011	60	DAI 2	3D imaging of transparent samples	Radochová Barbora, RNDr., Ph.D.
CellVizio Optic fibre confocal microscopy	2015	33	D 050	Optic fibre confocal microscope capable of fluorescence imaging from surface to deep tissues in whole animal. See more.	Otáhal Jakub doc. MUDr. PhD	Mgr. David Kala
Neuroimage - stereology microscope	2010	33	D 048	Neuroimage (MBF Bioscience)	Kubová Hana doc. Dr. DrSc.
Leica LMD 6000 Laser capture microdissector	2008	21	D 239	Allows to identify and dissect microareas from tissue or cell smear.	Martin Vodička Mgr.
Carv II system	2013	32	D 048	Spinning disc confocal microscope	Hadraba Daniel Mgr.	Mgr. David Vondrášek

For more information, you can also directly check the ProteoLab website.

	Year	Dept	Location	Use for	Manager	Operator
Mass spectrometer	2005	22	E 305	HPLC and mass spectrometry analysis of physiologically important compounds	Mikšík Ivan prof. Ing. DrSc.
Capillary electrophoresis Agilent 7100	2012	22	E 201	Separation instrument for analysis of a broad spectrum of compounds	Mikšík Ivan prof. Ing. DrSc.	Mgr. Pavla Sedláková, PhD
2D electrophoresis and Bruker MaXis MS	2009	22	E 306	Proteomic analysis	Mikšík Ivan prof. Ing. DrSc.	Ing. Statis Pataridis

Equipment	Year	Dept	Location	Use for	Manager	Operator
	Qtrap 5500 mass spectrometer	2012	17	E 218	metabolomics, lipidomics	Kuda Ondřej RNDr. Ph.D.	Ivana Brabcová, Ph.D.
	CentriVap Refrigerated Vacuum Concentrator	2016	17	E 218	metabolomics	Kuda Ondřej RNDr. Ph.D.

Equipment

Year

Dept

Location

Use for

Manager

Operator

2012

E 218

metabolomics, lipidomics

2016

E 218

metabolomics

Equipment		Year	Dept	Location	Use for	Manager	Operator

Behavioral analysis
	PhenoWorld - automatic modular system	2015	32	G	Modular system for complex behavioral analyses	Kubík Štěpán, RNDr. PhD.
	Multi conditioning system	2011	32	D 037	System accommodating nine standard conditioning paradigms for the evaluation of learning, memory, emotion and stress-related behaviors in mice and rats.	RNDr. Štěpán Kubík, Ph.D.
	Phenotyper - system for behavioral analysis	2013	33	D 011	PhenoTyper (Noldus) for 8 rats. System is designed for automatated behavioral analysis in instrumented observation cage.	Kubová Hana doc. Dr. DrSc.
	Hematology Analyzer scil Vet abc Plus+	2016	41	E 017	Analytical Instrument to determine haematological parameters from whole blood		RNDr. Světlana. Žufanová

CT/PET
	microCT/PET Albira	2013	96	E 013	In vivo imaging of small laboratory animals	Roman Liška

Echocardiography
	GE-VIVID 7 echocardiography	2010	80	D302b	Non-invasive assessment of heart geometry and function	Papoušek František Ing. CSc.

Blood pressure
	Telemetric monitoring of blood pressure	2013	25	D	Long term telemetric monitoring of blood pressure and sympathetic activity	Vaněčková Ivana RNDr. CSc.

Metabolism
	Indirect calorimetry INCA Somedic	2012	17	G 008	Indirect calorimetry for mice and rats	Kristína Bardová Mgr.

Equipment		Year	Dept	Location	Use for	Manager	Operator
	Oroboros Oxygraph 2k	2013	13+17	DAII 108	Three setups for measurement of cellular/mitochondrial respiration. One with fluorescent attachment for ROS/ATP monitoring	Pecina Petr, Mgr. PhD
	Seahorse Xfe-24 alanyzer	2014	13	DAII 228	Plate based system for measurement of respiration and glycolytic activity of cells	Mráček Tomáš, RNDr. PhD

	Year	Dept	Location	Use for	Manager
Agilent RNA analyzer	2004	21	D 224	Provides sizing, quantitation and quality control of DNA, RNA, proteins and cells on a single platform.	Ergang Peter Ing. PhD
Nanodrop spectrophotometer	2007	21	D 224	Measurement of nucleic acid concentration and purity and fluorescent dye labeled proteins.	Ergang Peter Ing. PhD
Light Cycler 480 real-time PCR	2009	17	D 224	Real-time PCR system
ViiA 7 real-time PCR	2012	21	D 224	Real-time PCR system with HRM possibility	Ergang Peter Ing. PhD

	Year	Dept	Location	Use for	Manager
Microbeta counter	2004	14	D 121	scintillation counting in a plate format	Doležal Vladimír MUDr. DrSc
Reader Tecan infinity M200	2007	13	DAII 112	Luminescence, Fluorescence (top reading), UV-absorbance (UV-VIS) plate-reader with injectors	Mráček Tomáš, RNDr. PhD
Reader Victor X4	2011	14	D 121	Luminescence, Fluorescence Intensity (both top and bottom reading), UV-absorbance (UV-VIS) and Time-Resolved Fluorescence technologies	Doležal Vladimír MUDr. DrSc
Reader Synergy HT	2008	16	D 324	single-channel absorbance, fluorescence (400/30, 460/40, 485/20, 508/20, 516/20, 528/20, 560/15, 590/20) and luminiscence microplate reader.	Zimmermannová Olga Ing. PhD

Equipment		Year	Dept.	Location	Use for	Manager	Operator
	Beckman Optima XL-100K	2001	60	D 024	floor model, max speed 100,000 rpm, rotors: 35 (retired), 45 Ti, 50.3 Ti, 70 Ti, 70.1 Ti, 90 Ti, NVT 90, SW 28, SW 41 Ti	Petr Svoboda
	Beckman Optima L-90	1996	13	DaII 114	floor model, max speed 90,000 rpm, rotors: same as for Optima XL-100K	Marek Vrbacky
	Beckman Optima MAX 130K	2007	60	E 318	benchtop model, max speed 130,000 rpm (1,019,000g with MLA-130 rotor), rotors: MLA-80, MLA-130, TLA-100, TLA-110, MLN-80	Petr Svoboda
	Beckman L8-55	1983	75	G 044	floor model, rotors: same as for Optima XL-100K	Tomáš Špaček
	Sorvall RC M100	1991	60	D 121	floor model, rotors: RP100AT3, RP100AT4, RP100VT	Petr Svoboda
Further info on Ultracentrifuges, Rotors, and Tubes