Čeština
EnglishAntisense oligodeoxynucleotide-mediated gene knockdown in pollen tubes.
Bezvoda R, Pleskot R, Žárský V, Potocký M.
Plant Cell Morphogenesis: Methods and Protocols 1080: 231-236, 2014
Keywords: Antisense oligodeoxynucleotide, AODN, Pollen tube, Gene knockdown, Tip growth
Abstract: Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
DOI: IEB authors: Roman Pleskot, Martin Potocký, Viktor Žárský
Plant Cell Morphogenesis: Methods and Protocols 1080: 231-236, 2014
Keywords: Antisense oligodeoxynucleotide, AODN, Pollen tube, Gene knockdown, Tip growth
Abstract: Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
DOI: IEB authors: Roman Pleskot, Martin Potocký, Viktor Žárský