Mravec J., Petrášek J., Li N., Boeren S., Karlova R., KitakuraS., Pařezová M., Naramoto S., Nodzynski T., Dhonukshe P., Bednarek S.Y., Zažímalová E., de Vries S., Friml J.
CURRENT BIOLOGY 21: 1055-1060, 2011

Klíčová slova: dynamin, auxin, PIN, polarity
Abstrakt: The polarized transport of the phytohormone auxin, which is crucial for the regulation of different stages of plant development, depends on the asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers. The PIN polar localization results from clathrin-mediated endocytosis (CME) from the plasma membrane and subsequent polar recycling. The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin-a protein required for fission of endocytic vesicles during CME. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence complementation (BiFC), and Forster resonance energy transfer (FRET) that members of the DRP1 group closely associate with PIN proteins at the cell plate. Localization and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1 function in correct PIN distribution and in auxin-mediated development. We propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells.
DOI: Autoři z ÚEB: Markéta Fílová, Jan Petrášek, Eva Zažímalová