Organic Chemistry

Tutor	Prof. Ing. Michal Hocek, PhD. DSc.
Team	Michal Hocek
Topic	Modifications of nucleotides and RNA by reactive groups for bioconjugations and cross-links
Faculty	FCHT or FPBT VŠCHT, PřF UK
Abstract	Ribonucleotides and RNA bearing reactive functional groups will be designed and synthesized. The modified RNAs will be prepared by polymerase incorporations of modified nucleoside triphosphates or by click reactions on RNA. Bioorthogonal reactions of these groups in RNA will be studied, e.g. cross-linking with proteins, attachment of other useful functional groups and applications in chemical biology and diagnostics. Leading references: 1. Dadová, J.; Orság, P.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Vinylsulfonamide and acrylamide modification of DNA for cross-linking with proteins" Angew. Chem. Int. Ed. 2013, 52, 10515-10518. 2. Milisavljevič, N.; Perlíková, P.; Pohl, R.; Hocek, M.: "Enzymatic synthesis of base-modified RNA by T7 RNA polymerase. A systematic study and comparison of 5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside triphosphates as substrates" Org. Biomol. Chem. 2018, 16, 5800-5807