Introduction Department Cellular Neurophysiology

Cellular Neurophysiology

Electrophysiological recording of glutamate-induced current responses in cultured hippocampal neurons.

Our research is mainly directed to exploration of functional and pharmacological properties of ion channels, including those activated during synaptic transmission. For that purpose, we use electrophysiological methods, primarily patch clamp technique, combined with molecular-biology, immunohistochemical and microfluorometric methods (Ca²⁺imaging). Using this approach, we study properties of excitable cells, such as cultured hippocampal and granular neurons or in vitro preserved neural circuits of pyramidal neurons in the hippocampal brain slices, as well as HEK293 and COS cell lines transfected with recombinant NMDA or vanilloid receptors. The goal of our research is to comprehend the relationship between structure and function of ion channels, to characterize the actions of pharmacologically active substances and to explain the molecular mechanism by which they influence ligand-gated ion channels.

For more detailed understanding of physiological mechanisms and pharmacology of ligand-gated ion channels, we use quantitative analytical techniques (including the analysis of single ion channels, measuring of intracellular concentrations of Ca2+ and mathematical modeling).

History of department

Methods

Alumni list

Department´s web

Projects

Research on glutamate receptors

Glutamate is the main excitatory neurotransmitter in the central nervous system. It activates specific membrane proteins known as glutamate receptors. Our studies focus on ionotropic glutamate receptors, which, when activated, allow ions to flow across the cell membrane. More

Research on TRP channels

We investigate molecular mechanisms of nociception and thermosensation by focusing on transient receptor potential (TRP) ion channels (Figure). These channels are specifically expressed in primary nociceptive neurones and work in concert to detect potentially damaging stimuli and transduce them into pain signalling. More

Publications

Zímová, Lucie - Sinica, Viktor - Kádková, Anna - Vyklická, Lenka - Zíma, Vlastimil - Barvík, I. - Vlachová, Viktorie . Intracellular cavity of sensor domain controls allosteric gating of TRPA1 channel . Science Signaling 2018, roč. 11, 514, článku eaan8621 . IF = 6.378 [ASEP] [ doi ]

Vyklický, Vojtěch - Krausová, Barbora - Černý, Jiří - Ladislav, Marek - Smejkalová, Tereza - Kysilov, Bohdan - Kořínek, Miloslav - Danačíková, Šárka - Horák, Martin - Chodounská, Hana - Kudová, Eva - Vyklický ml., Ladislav . Surface Expression, Function, and Pharmacology of Disease-Associated Mutations in the Membrane Domain of the Human GluN2B Subunit . Frontiers in Molecular Neuroscience 2018, roč. 11, Apr 6, článku 110 . IF = 3.902 [ASEP] [ doi ]

Touška, Filip - Turnquist, B. - Vlachová, Viktorie - Reeh, P. W. - Leffler, A. - Zimmermann, K. Heat-resistant action potentials require TTX-resistant sodium channels Na(V)1.8 and Na(V)1.9 . Journal of General Physiology 2018, roč. 150, 8, p. 1125-1144 . IF = 3.680 [ASEP] [ doi ]

Skřenková, Kristýna - Lee, S. - Lichnerová, Katarina - Kaniaková, M. - Hansíková, H. - Zápotocký, Martin - Suh, Y. H. - Horák, Martin . N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors . Frontiers in Molecular Neuroscience 2018, roč. 11, Jun 4, článku 188 . IF = 3.902 [ASEP] [ doi ]

Průcha, J. - Krůšek, Jan - Dittert, Ivan - Sinica, Viktor - Kádková, Anna - Vlachová, Viktorie . Acute exposure to high‐induction electromagnetic field affects activity of model peripheral sensory neurons . Journal of Cellular and Molecular Medicine 2018, roč. 22, 2, p. 1355-1362 . IF = 4.302 [ASEP] [ doi ]