Biologia plantarum - Latest articles
Results 181 to 210 of 232:
Soybean NAC gene family: sequence analysis and expression under low nitrogen supplyOriginal Papers
X. Wang, D. Li, J. Jiang, Z. Dong, Y. Ma
Biologia plantarum 2017, 61:473-482 | DOI: 10.1007/s10535-016-0693-4
NAM, ATAF1/2, and CUC2 (NAC) proteins are plant-specific transcription factors playing essential roles in plant development and various abiotic stress responses. In the present study, we identified 173 full-length NAC genes in soybean, which were phylogenetically clustered into 15 groups (NACa - NACo). The soybean NAC genes (GmNACs) were non-randomly located across the 20 chromosomes, and 128 genes (86.5 %) were preferentially located in duplicated regions of chromosome arms, which implied long segmental duplication and contributed to evolution of the GmNAC gene family. Most GmNACs genes showed a distinct...
Selection of reference genes for quantitative real-time PCR in Casuarina equisetifolia under salt stressOriginal Papers
C. Fan, Z. Qiu, B. Zeng, Y. Liu, X. Li, G. Guo
Biologia plantarum 2017, 61:463-472 | DOI: 10.1007/s10535-016-0670-y
Real time quantitative PCR (qPCR) is widely used in gene expression analysis for its accuracy and sensitivity. Reference genes serving as endogenous controls are necessary for gene normalization. In order to select an appropriate reference gene to normalize gene expression in Casuarina equisetifolia under salt stress, 10 potential reference genes were evaluated using real time qPCR in the leaves and roots of plants grown under different NaCl concentrations and treatment durations. GeNorm, NormFinder, and BestKeeper analyses reveal that elongation factor 1-alpha (EF1α) and ubiquitin-conjugating enzyme...
Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheatOriginal Papers
E. Peñaloza, M. Santiago, S. Cabrera, G. Muñoz, L. J. Corcuera, H. Silva
Biologia plantarum 2017, 61:453-462 | DOI: 10.1007/s10535-016-0672-9
The root specificity and phosphate (Pi) deficiency responsiveness of high-affinity phosphate transporter (PHT1) genes point to their promoters as a sustainable system to drive Pi acquisition-related transgenes in plants. In this study, a 3-kb promoter of the AtPHT1;4 gene from Arabidopsis thaliana fused to the β-glucuronidase (GUS) reporter gene was biolistically introduced into wheat (Triticum aestivum L.) and functionally characterized in transgenic plants grown in hydroponics and in pots with soil under various Pi supply rates. From among 27 T1 progeny derived from 250 T0, four transgenic...
Genome-wide identification, classification, and expression analysis of the phytocyanin gene family in Phalaenopsis equestrisOriginal Papers
L. Xu, X. J. Wang, T. Wang, L. B. Li
Biologia plantarum 2017, 61:445-452 | DOI: 10.1007/s10535-017-0716-9
Phytocyanins (PCs) are ancient blue copper-binding proteins in plants that bind to single type I copper atoms and function as electron transporters. PCs play an important role in plant development and stress resistance. Many PCs are considered to be chimeric arabinogalactan proteins (AGPs). Previously, 38, 62, and 84 PC genes were identified in Arabidopsis thaliana, Oryza sativa, and Brassica rapa, respectively. In this study, we identified 30 putative PC genes in the orchid Phalaenopsis equestris through comprehensive bioinformatics analysis. Based on phylogeny and motif constitution, the P. equestris...
Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonateOriginal Papers
Z. Q. Yuan, J. Y. Zhang, T. Liu
Biologia plantarum 2017, 61:438-444 | DOI: 10.1007/s10535-016-0702-7
The accumulation of polysaccharides, activities of sucrose metabolism enzymes, and the expression of sucrose biosynthetic genes in Dendrobium officinale were significantly affected by exogenous methyl jasmonate (MeJA). Application of MeJA increased the content of polysaccharides and the highest polysaccharide production occurred in the samples treated with 200 μM MeJa. The MeJA application influenced polysaccharide biosynthesis rather than degradation because the activities of sucrose metabolism enzymes and the expressions of sucrose biosynthetic genes were upregulated by MeJA. Interestingly, low MeJA concentrations promoted accumulation...
In vitro propagation, microtuberization, and molecular characterization of three potato cultivarsOriginal Papers
J. Salem, A. M. Hassanein
Biologia plantarum 2017, 61:427-437 | DOI: 10.1007/s10535-017-0715-x
Sprouts of potato tubers were excised from the three potato cultivars Agria, Hermes, and Spunta, sterilized and subjected to shoot formation and propagation on Murashige and Skoog (MS) medium supplemented with 1 mg dm-3 6-benzylaminopurine (BAP) + 0.5 mg dm-3 gibberellic acid. Shoots were rooted on MS medium supplemented with 1 mg dm-3 indole-3-butyric acid. To increase shoot vigour prior tuber formation, shoots were subcultured on MS medium supplemented with 0.56 mg dm-3 BAP, 0.11 mg dm-3 2,4-dichlorophenoxyacetic acid, and 0.96 mg dm-3 naphthaleneacetic acid. Under dark, microtuberization...
Cytosolic GAPDH: a key mediator in redox signal transduction in plantsReviews
S. S. Yang, Q. H. Zhai
Biologia plantarum 2017, 61:417-426 | DOI: 10.1007/s10535-017-0706-y
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serves not only as a key enzyme in glycolysis, but also as a multifunctional protein in other biological processes, especially in response to abiotic stresses in plants. Cytosolic GAPDH (GAPC) is a typical redox protein with selected catalytic cysteine, which undergoes reversible redox post-translational modifications (RPTMs) on its thiol group by reacting with hydrogen peroxide and nitric oxide related species. Moreover, the modified GAPC may interact with certain signal transmitters such as phosphatidic acid, phospholipase D, and osmotic stress-activated protein kinase. All these observations suggest...
Signal transduction and biotechnology in response to environmental stressesReviews
M. A. Ahanger, N. A. Akram, M. Ashraf, M. N. Alyemeni, L. Wijaya, P. Ahmad
Biologia plantarum 2017, 61:401-416 | DOI: 10.1007/s10535-016-0683-6
Providing sufficient food to burgeoning population from the steadily shrinking arable land seems to be very difficult in near future and is one of the foremost challenges for plant scientists. In addition, there are several biotic and abiotic stresses which frequently encounter crop plants during various stages of life cycle, resulting in considerable yield losses. Environmental stresses, including drought, flooding, salinity, temperature (both low and high), high radiation, and xenobiotics induce toxicity, membrane damage, excessive reactive oxygen species (ROS) production, reduced photosynthesis, and altered nutrient acquisition. Several indigenous...
Changes in dehydrin composition in winter cereal crowns during winter survivalBrief Communications
A. V. Pomortsev, N. V. Dorofeev, N. B. Katysheva, A. A. Peshkova
Biologia plantarum 2017, 61:394-398 | DOI: 10.1007/s10535-016-0673-8
This study is focused on examination of crown dehydrin content during overwintering and spring dehardening periods in three Poaceae family winter plants: rye, wheat, and triticale. Frost resistances of seedlings in laboratory and field conditions were compared. Immunoblotting demonstrates that winter wheat and winter triticale differed from winter rye based on their dehydrin qualitative content. Unlike wheat and triticale, rye lacked a protein with a molecular mass of 55.3 kDa. Winter wheat contained a polypeptide with a molecular mass of 29 kDa in autumn but lacked it in winter compared with triticale. Comparison of dehydrin spectra from the...
Transcriptional properties of eight synthetic pathogen-inducible promoters in transgenic Arabidopsis thalianaBrief Communications
Z. C. Huang, S. Peng, H. Li, F. H. Zeng
Biologia plantarum 2017, 61:389-393 | DOI: 10.1007/s10535-016-0665-8
Synthetic pathogen-inducible promoters (SPIP) hold a great promise to meet the demands for a desired temporal and spatial regulation of transgenes. Four pathogen-inducible cis-elements (F-box, S-box, Gst1-box, and W-box) and the minimal cauliflower mosaic virus 35S (CaMV 35S) promoter (-46 to +8 TATA box) were used to design SPIP. Eight SPIP were synthesized and named FSGW, FSWG, GWFS, GWSF, SFGW, SFWG, WGFS, and WGSF according to the order of cis-element dimers. They were used to replace the CaMV 35S promoter in the plasmid pBI121 to control expression of the β-glucuronidase (gus) gene. The transcriptional properties...
Inhibition of putrescine biosynthesis enhanced salt stress sensitivity and decreased spermidine content in rice seedlingsBrief Communications
A. Yamamoto, I.-S. Shim, S. Fujihara
Biologia plantarum 2017, 61:385-388 | DOI: 10.1007/s10535-016-0676-5
The effect of polyamine biosynthesis inhibitors on the salt stress response of rice seedlings was investigated. For this, DL-α-difluoromethylarginine (DFMA) and DL-α-difluoromethylornithine (DFMO), two competitive inhibitors of arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), were used. The ADC and ODC are rate-limiting enzymes involved in synthesis of putrescine. The effective quantum yield of photosynthetic energy conversion (ΦPSII) decreased with the salt stress, and this decrease was highly significant in the treatments with DFMA and DFMO. Interestingly, addition of exogenous putrescine reduced the decline...
Microwaves affect Myriophyllum aquaticum plants differently depending on the wave polarizationOriginal Papers
M. D. H. J. Senavirathna, T. Asaeda
Biologia plantarum 2017, 61:378-384 | DOI: 10.1007/s10535-016-0660-0
Previous studies on microwave exposure on plants have revealed variations in sensitivity of plants to different microwave frequencies, exposure durations, and power intensities. However, the effects of different polarizations of microwaves on plants have not been studied. Therefore, we investigated the effect of horizontally and vertically polarized 2 GHz continuous microwaves on Myriophyllum aquaticum plants at 1.8 W m-2 power density. The electric potential variation along the vascular tissues were investigated for 1.5 h and growth parameters, pigmentation, and H2O2 formation were studied during 48 h microwave...
Protection of Artemisia annua roots and leaves against oxidative stress induced by arsenicOriginal Papers
A. Kumari, N. Pandey, S. Pandey-Rai
Biologia plantarum 2017, 61:367-377 | DOI: 10.1007/s10535-016-0686-3
The present study was conducted to examine differential responses of roots and leaves of Artemisia annua to different arsenic concentrations (50, 100, and 150 μΜ) and treatment durations (1, 3, 5, or 7 d). The values of bioconcentration factor and translocation factor calculated on the basis of total As-accumulation in roots and shoots suggested that A. annua is a good As-accumulator. Above and below ground plant biomass was enhanced at 100 μΜ As but at 150 μΜ As was significantly reduced. As-treatment caused membrane damage more in the roots than in the leaves as reflected by higher degree of lipid peroxidation in the...
Differential proline metabolism in vegetative and reproductive tissues determine drought tolerance in chickpeaOriginal Papers
D. Kaur, S. K. Grewal, J. Kaur, S. Singh
Biologia plantarum 2017, 61:359-366 | DOI: 10.1007/s10535-016-0695-2
Proline is emerging as a critical component of drought tolerance and fine tuning of its metabolism under stress affects the plants sensitivity and response to stress. Thus the study was carried out to analyse the effect of water deficit on the proline content and principal enzymes involved in its synthesis (Δ1-pyrolline-carboxylate synthetase) and catabolism (proline dehydrogenase) at different developmental stages and in different organs (roots, nodules, leaves, pod wall, and seeds) of two chickpea (Cicer arietinum L.) cultivars differing in drought tolerance (drought tolerant ICC4958 and drought sensitive ILC3279). It was...
Altered gibberellin content affects growth and development in transgenic tobacco lines overexpressing a wheat gene encoding F-box proteinOriginal Papers
S. Yin, S. Zhou, X. Kong, Y. Han, W. Wang
Biologia plantarum 2017, 61:349-358 | DOI: 10.1007/s10535-017-0707-x
In a previous study, we have identified and characterized gene from wheat (Triticum aestivum L.) encoding F-box protein and named it TaFBA. In this paper, transgenic tobacco (Nicotiana tabacum L.) plants overexpressing TaFBA1 displayed accelerated growth early, but the rate slowed gradually at later stages of growth, and the mature transgenic plants were even shorter in stature and flowered later than did the wild type (WT). Treatment with gibberellin (GA) conferred an accelerated growth rate to the transgenic tobacco plants at later stages, similar to that of WT, whereas growth was inhibited more seriously in WT than in...
Embryo lethality in wheat-rye hybrids: dosage effect and deletion bin mapping of the responsible wheat locusOriginal Papers
N. Tikhenko, N. Poursarebani, T. Rutten, T. Schnurbusch, A. Börner
Biologia plantarum 2017, 61:342-348 | DOI: 10.1007/s10535-016-0691-6
The speciation allele at Eml-A1 of hexaploid wheat, which causes embryo lethality in wheat-rye hybrids, was investigated using cytologically modified genetic stocks. It was demonstrated that an extra dose of this allele had no effect on embryo development in these hybrids. There was no positive effect on embryo development and, therefore, no overcoming of the postzygotic barrier. An abortion of the hybrid embryos at an earlier stage of development was also not observed. Physical mapping was performed using chromosome 6A deletion lines. This study revealed the location of Eml-A1 on the most distal part of the long arm of chromosome 6A....
Changes in antioxidant enzyme activities and gene expression in two muskmelon genotypes under progressive water stressOriginal Papers
W. A. Ansari, N. Atri, B. Singh, S. Pandey
Biologia plantarum 2017, 61:333-341 | DOI: 10.1007/s10535-016-0694-3
Responses of two muskmelon (Cucumis melo L.) genotypes (drought tolerant SC-15 and drought susceptible EC-564755) were analyzed at 0, 7, 14, and 21 d of progressive water stress. Although water deficit caused a significant decline in relative water content, the magnitude of reduction was lower in SC-15. Electrolyte leakage, hydrogen peroxide, and malonydialdehyde generation were higher in EC-564755, whereas accumulation of proline was higher in SC-15. Higher activities of antioxidant enzymes, such as catalase, superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and glutathione reductase, and higher expression of the respective genes...
Enhancement of stress tolerance in cucumber seedlings by proanthocyanidinsOriginal Papers
L.-J. Zhu, X.-G. Deng, L.-J. Zou, D.-W. Zhang, H.-H. Lin
Biologia plantarum 2017, 61:323-332 | DOI: 10.1007/s10535-016-0663-x
Proanthocyanidins (PAs) are the main products of the flavonoid biosynthetic pathway in many plants. However, their biological function during environmental stresses in plants is rarely reported. In the present study, the effects of pretreatment with PAs on the response of cucumber (Cucumis sativus L.) seedlings to high irradiance (HI), polyethylene glycol (PEG), and cold stress were investigated. The PAs pretreament alleviated stress-induced oxidative damage in plant cells and increased the activity of alternative oxidase (AOX) and content of abscisic acid (ABA). Furthermore, PAs-pretreated seedlings suffered less damage by the stress conditions,...
Low temperature-induced leaf senescence and the expression of senescence-related genes in the panicles of Litchi chinensisOriginal Papers
H.-F. Yang, X.-Y. Lu, H.-B. Chen, C.-C. Wang, B.-Y. Zhou
Biologia plantarum 2017, 61:315-322 | DOI: 10.1007/s10535-016-0667-6
Litchi is one of the most important subtropical evergreen fruit trees in Southern Asia. Litchi floral buds are a mix of axillary or apical panicle primordia, leaf primordia, and rudimentary leaves. Under usual winter and early spring conditions, the axillary panicle primordia prevail, and the rudimentary leaves abscise when low temperatures reach a certain threshold. The floral buds ultimately develop into pure panicles. Understanding the regulatory mechanism of rudimentary leaf senescence is of great importance for litchi flowering. In this study, litchi potted trees at the floral differentiation stage were treated with low and high temperatures in...
Anatomy, photochemical activity, and DNA polymorphism in leaves of dwarf tomato irradiated with X-raysOriginal Papers
C. Arena, M. Turano, B. Hay Mele, P. R. Cataletto, M. Furia, M. Pugliese, V. De Micco
Biologia plantarum 2017, 61:305-314 | DOI: 10.1007/s10535-016-0668-5
The response of higher plants to ionising radiation depends on factors related to both radiation properties and plant features including species, cultivar, age, and structural complexity of the target organ. Adult plants of dwarf tomato were irradiated with different doses of X-rays to investigate possible variations in leaf morpho-anatomical traits, photosynthetic efficiency, and genomic DNA. In order to assess if and how responses depend on leaf developmental stage, we analysed two types of leaves; nearly mature leaves (L1) and actively developing leaves (L2), whose lamina size corresponded to 70 and 25 %, respectively, of the lamina size of the...
Transcriptome-wide identification and expression analyses of ABC transporters in dwarf polish wheat under metal stressesOriginal Papers
X. Wang, C. Wang, H. Sheng, Y. Wang, J. Zeng, H. Kang, X. Fan, L. Sha, H. Zhang, Y. Zhou
Biologia plantarum 2017, 61:293-304 | DOI: 10.1007/s10535-016-0697-0
ABC transporters, which comprise one of the largest protein families, are involved in maintaining osmotic homeostasis, nutrient uptake, pathogen resistance, and metal tolerance. In this study, 30 ABC genes in dwarf polish wheat were characterized and classified into seven subfamilies (ABCA - ABCG). Among them, 24 ABC transporters were newly found in wheat. The expressions of 13 ABC genes in roots and leaves under six metal stresses were also analyzed. All these genes were differentially regulated by Cd (except ABCE2, ABCF4, and ABCF6 in roots), suggesting that these genes participate in Cd transport, sequestration,...
Function of Malus prunifolia WRKY6 transcription factor in response to different stressesOriginal Papers
N. Wang, Z.-Y. Yue, P. Wang, X. Sun, X.-Q. Gong, F.-W. Ma
Biologia plantarum 2017, 61:284-292 | DOI: 10.1007/s10535-016-0701-8
The WRKY transcription factors (TFs) are integral parts of signaling pathways that regulate many processes, such as senescence, seed dormancy, seed germination, and resistance to abiotic and biotic stresses. Stress-related functions of WRKY6 have been characterized in Arabidopsis and other plant species, but its role has not been identified in apple. Here, we cloned WRKY6 genes from Malus prunifolia. Two homologues MpWRKY6a and MpWRKY6b found in this species were members of Group II WRKY6 TFs. They were localized to the cell nucleus. MpWRKY6a can bind to W-boxes. Compared with the untransformed wild type plants, MpWRKY6a-overexpressing...
The analysis of mutant phenotypes and tissue expression reveals a role of SNAREs VAMP721 and VAMP722 in seedling growthOriginal Papers
L. Zhang, H. Y. Zhao, W. C. Qi, F. X. Zheng, T. Q. Wang, J. Y. Li
Biologia plantarum 2017, 61:275-283 | DOI: 10.1007/s10535-017-0745-4
Membrane traffic mediated by a soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) complex contributes to plant growth and development. However, the functional significance of SNAREs involved in cell wall deposition and seedling development has not been sufficiently explored. In this study, we explored the roles of R-SNAREs VAMP721 (At1g04750) and VAMP722 (At2g33120) in seedling growth of Arabidopsis thaliana by histochemical staining, fluorescence labeling, and analyzing mutant phenotypes. Our results show a massive intracellular accumulation of cellulose and callose, and an abnormal deposition of callose...
Characterization and functional analysis of transcription factor ZmEIL1 in maizeOriginal Papers
Q. L. Shi, Y. B. Dong, D. H. Qiao, Q. Zhou, L. Zhang, Z. Y. Ma, Y. L. Li
Biologia plantarum 2017, 61:266-274 | DOI: 10.1007/s10535-017-0705-z
As key nuclear transcription factors, the ethylene-insensitive3/EIN3-like (EIN3/EIL) proteins play important roles in ethylene signal transduction pathway in various plants. In order to better understand the role of EIN3/EILs, one EIN3-like gene (designated ZmEIL1) was isolated from maize (Zea mays L.). The full-length cDNA of ZmEIL1 was 1 999 bp in length and encoded 647 amino acids. Sequence comparison of ZmEIL1 protein with other EIN3/EILs proteins revealed high conservation of five α-helices that could form a V-shaped cleft in a 3-D model, just like AtEIL3 in Arabidopsis thaliana. This protein showed...
Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther cultureOriginal Papers
P. Zhao, K. Wang, W. Zhang, H. Y. Liu, L. P. Du, H. R. Hu, X. G. Ye
Biologia plantarum 2017, 61:255-265 | DOI: 10.1007/s10535-016-0662-y
The anther culture technique, although it has been widely used in wheat breeding programs, is associated with a high albinism rate that limits its application. The mechanism of albinism has not been studied explicitly at the molecular level. In this study, differently expressed genes (DEGs) and proteins (DEPs) of albino and green seedlings from a wheat anther culture were analyzed and compared using transcriptomic sequencing (RNA-Seq), two-dimensional electrophoresis, and mass spectrometry. A total of 1 892 DEGs (1 115 down-regulated and 777 up-regulated) and 43 DEPs (15 down-regulated and 28 up-regulated) that are primarily involved in photosynthetic...
Gene expression and flavonol biosynthesis are induced by ultraviolet-B and salt stresses in Reaumuria trigynaOriginal Papers
H. Zhang, Z. Wu, Y. Suo, J. Wang, L. Zheng, Y. Wang
Biologia plantarum 2017, 61:246-254 | DOI: 10.1007/s10535-017-0725-8
In plants, flavonoids play roles not only in development, but also in responses to biotic and abiotic stresses. We analyzed the transcriptome data of NaCl-treated Reaumuria trigyna, a small, highly haloduric desert shrub, focusing on the flavonoid biosynthetic pathway. We identified 118 unigenes annotated as genes encoding enzymes related to flavonoid biosynthesis, 68 of which were differentially expressed under NaCl treatment (39 upregulated, 29 downregulated). Of the 118 annotated unigenes, 47 were annotated as members of families related to the flavonol biosynthetic pathway (e.g., F3H, FLS, and OMT). Of those 47...
An intronless sucrose:fructan-6-fructosyltransferase (6-SFT) gene from Dasypyrum villosum enhances abiotic tolerance in tobaccoOriginal Papers
X. L. He, J. W. Wang, W. X. Li, Z. Z. Chen, J. Wu, J. X. Zhao, J. N. Su, Z. H. Wang, X. H. Chen
Biologia plantarum 2017, 61:235-245 | DOI: 10.1007/s10535-016-0696-1
Fructans play vital roles in enhancing plant abiotic stress tolerance by reducing oxidative damage, stabilizing cell membranes, improving the osmotic adjustment capacity, and lowering the freezing point. In this study, a sucrose: fructan-6-fructosyltransferase (6-SFT) gene involved in the synthesis of fructans was isolated from Dasypyrum villosum, Dv-6-SFT, using genomic walking and reverse transcription (RT)-PCR. Alignment of the cDNA sequence with its genomic counterpart showed that no introns were present in the Dv-6-SFT gene, and thus it differs from all other plant 6-SFTs that have been cloned previously. Sequence...
Differential expression pattern of transcription factors across annual Medicago genotypes in response to salinity stressOriginal Papers
F. Mokhtari, F. Rafiei, L. Shabani, B. Shiran
Biologia plantarum 2017, 61:227-234 | DOI: 10.1007/s10535-016-0666-7
Plants respond differently to salinity stress due to their unique gene architectures. Among genes, transcription factors (TFs) regulate many physiological and biochemical processes by modulating the rate of transcription initiation of target genes. Modulation of TFs has been correlated to the salt adaptation of any given genotype. In order to identify the expression of eight TFs (belong to bHLH, CBF, MYB, WRKY, and Zpt2 families) in three annual Medicago genotypes (M. polymorpha cv. Ieze, M. laciniata cv. Shushtar, and M. laciniata cv. Gheshm) under salinity stress, the RT-qPCR analyses were performed. Attempts were also...
Expression of sucrose metabolism and transport genes in cassava petiole abscission zones in response to water stressOriginal Papers
W. B. Liao, Y. Y. Li, C. Lu, M. Peng
Biologia plantarum 2017, 61:219-226 | DOI: 10.1007/s10535-016-0658-7
Cassava (Manihot esculenta Crantz) is an important crop, and its starch formation is regulated by sucrose metabolism and transport. To understand the roles of sucrose metabolism and transport in cassava under water stress, we studied not only sucrose metabolism and transport in cassava abscission zones (AZs) but also expression of respective genes. Sucrose was transported from leaves to roots in the early stage of water stress, and a reverse sucrose flow was detected in the later stages of the stress. The decrease in sucrose content was related to leaf senescence and inhibition of photosynthesis. Microarray analyses showed seven genes encoding...
De novo transcriptome analysis of Fraxinus velutina using Illumina platform and development of EST-SSR markersOriginal Papers
L.-P. Yan, C.-L. Liu, D.-J. Wu, L. Li, J. Shu, C. Sun, Y. Xia, L.-J. Zhao
Biologia plantarum 2017, 61:210-218 | DOI: 10.1007/s10535-016-0681-8
To facilitate functional genomic analysis and molecular breeding of velvet ash (Fraxinus velutina Torr), the de novo sequencing was carried out by Illumina sequencing technology. The cDNA samples were prepared from eleven different tissues of velvet ash and sequenced by using the Illumina genome analyzer. Subsequently, de novo assemebly, functional annotation databases, and the screening of expressed sequence tag-simple sequence repeats (EST-SSRs) were performed by comparing with corresponding databases using BLASTx and software tools. We obtained 51 698 unigenes with an average length of 661 bp and an N50...