Biologia plantarum 2014, 58:164-168 | DOI: 10.1007/s10535-013-0369-2

Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora

G. Salcedo-Morales1,2, A. R. Jiménez-Aparicio2, F. Cruz-Sosa1, G. Trejo-Tapia2,*
1 Departamento de Biotecnología, Universidad Autónoma Metropolitana-Unidad Iztapalapa, México, D.F., México
2 Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, México

In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 μM catechin, 25 μM vanillin, or 25 μM H2O2. Of the treatments tested, 25 μM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids.

Keywords: catechin; flavonoids; hemiparasitism; hydrogen peroxide; vanillin
Subjects: in vitro culture; hemiparasitism; haustorium; catechin; flavonoids; hydrogen peroxide; vanillin; growth analysis
Species: Castilleja tenuiflora

Received: August 25, 2012; Revised: April 30, 2013; Accepted: July 2, 2013; Published: March 1, 2014Show citation

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Salcedo-Morales, G., Jiménez-Aparicio, A.R., Cruz-Sosa, F., & Trejo-Tapia, G. (2014). Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora. Biologia plantarum, 58(1), 164-168. doi: 10.1007/s10535-013-0369-2.
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    References

    1. Albrecht, H., Yoder, I., Phillips, D.: Flavonoids promote haustoria formation in the root parasite Triphysaria versicolor. - Plant Physiol. 119: 585-591, 1999. Go to original source...
    2. Baird, V., Riopel, J.: Experimental studies of haustorium initiation and early development in Agalinis purpurea (L.) Raf. (Scrophulariaceae). - Amer. J. Bot. 71: 803-814, 1984. Go to original source...
    3. Bancroft, J., Stevens, A.: Theory and Practice of Histological Techniques. 2nd Ed. - Churchill Livingstone, New York 2002.
    4. Cardoso, C., Ruyter, S., Bouwmeester, H.: Strigolactones and root infestation by plant-parasitic Striga, Orobanche and Phelipanche spp. - Plant Sci. 180: 414-420, 2011. Go to original source...
    5. Deeks, S., Shamoun, S., Punja, Z.: Tissue culture of parasitic flowering plants: methods and applications in agriculture and forestry. - In Vitro cell. dev. Biol. Plant. 35: 369-381, 1999. Go to original source...
    6. Dobbins, R., Kuijt, J.: Studies on the haustorium of Castilleja (Scrophulariaceae). I. The upper haustorium studies on the haustorium of Castilleja (Scrophulariaceae). I. The upper haustorium. - Can J. Bot. 51: 917-922, 1973. Go to original source...
    7. Gómez-Aguirre, Y., Zamilpa, A., González-Cortazar, M., Trejo-Tapia, G.: Adventitious root cultures of Castilleja tenuiflora Benth. as a source of phenylethanoid glycosides. - Ind. Crops Prod. 36: 188-195, 2012. Go to original source...
    8. Hood, M., Condon, J., Timko, M., Riopel, J.: Primary haustorial development of Striga asiatica on host and nonhost species. - Phytopathology 88: 70-75, 1998. Go to original source...
    9. Huang, A., She, X., Cao, B., Ren, Y.: Distribution of hydrogen peroxide during adventitious roots initiation and development in mung bean hypocotyls cuttings. - Plant Growth Regul. 64: 109-118, 2011. Go to original source...
    10. Jamison, D., Yoder, J.: Heritable variation in quinone induced haustorium development in the parasitic plant Triphysaria. - Plant Physiol. 125: 1870-1879, 2001.
    11. Jiménez, M., Padilla, M., Reyes, Ch., Espinosa, L., Melendez, E., Lira, A.: Iridoid glycoside constituents of Castilleja tenuiflora. - Biochem. Syst. Ecol. 23: 455-456, 1995.
    12. Kärk, A., Koutaniemi, S.: Lignin biosynthesis studies in plant tissue cultures. - J. integr. Plant Biol. 52: 176-185, 2010.
    13. Keyes, W., O'Malley, R., Kim, D., Lynn, D.: Signaling organogenesis in parasitic angiosperms: xenognosin generation, perception, and response. - J. Plant Growth Regul. 19: 217-223, 2000.
    14. Keyes, W., Palmer, A., Erbil, W., Taylor, J., Apkarian, R., Weeks, E., Lynn, D.: Semagenesis and the parasitic angiosperm Striga asiatica. - Plant J. 51: 707-716, 2007. Go to original source...
    15. Keyes, W., Taylor, J., Apkarian, R., Lynn, D.: Dancing together: social controls in parasitic plant development. - Plant Physiol. 127: 1508-1512, 2001. Go to original source...
    16. Kotis, M., Yupsanis, T., Syros, T., Economou, A.: Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia × hybrid microshoots. - Biol. Plant. 53: 530-538, 2009. Go to original source...
    17. Lara, A., Valverde, R., Gómez, L.: [Histology of somatic embryos and adventitious shoots induced from leaves of Psychotria acuminata.] Histología de embriones somáticos y brotes adventicios inducidos en hojas de Psychotria acuminata. - Agron. Costarricense 27: 37-48, 2003. [In Span.]
    18. Lee, K.: Structure and development of the upper haustorium in the parasitic flowering plant Cuscuta japonica (Convolvulaceae). - Amer. J. Bot. 94: 737-745, 2007. Go to original source...
    19. López-Laredo, A., Gómez-Aguirre, Y., Medina-Pérez, V., Salcedo-Morales, G., Sepúlveda-Jiménez, G., Trejo-Tapia, G.: Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth. - Acta Physiol. Plant. 34: 2435-2442, 2012. Go to original source...
    20. Lu, J., Li, X.N., Yang, Y.L., Jia, L.Y., You, J., Wang, W.R.: Effect of hydrogen peroxide on seedling growth and antioxidants in two wheat cultivars. - Biol. Plant. 57: 487-484, 2013. Go to original source...
    21. Martínez-Bonfil, B., Salcedo-Morales, G., López-Laredo, A., Ventura-Zapata, E., Evangelista-Lozano, S., Trejo-Tapia, G.: Shoot regeneration and determination of iridoid levels in the medicinal plant Castilleja tenuiflora Benth. - Plant Cell Tissue Organ Cult. 107: 195-203, 2011. Go to original source...
    22. Molassiotis, A., Dimassi, K., Diamantdis, G., Therios, I.: Changes in peroxidases and catalase activity during in vitro rooting. - Biol. Plant. 48: 1-5, 2004. Go to original source...
    23. O'Malley, R., Lynn, D.: Expansin message regulation in parasitic angiosperms: marking time in development. - Plant Cell Online 12: 1455-1465, 2000. Go to original source...
    24. Rani, A., Kumar Vats, S., Sharma, M., Kumar, S.: Catechin promotes growth of Arabidopsis thaliana with concomitant changes in vascular system, photosynthesis and hormone content. - Biol. Plant. 55: 779-782, 2011. Go to original source...
    25. Riopel, J., Musselman, L.: Experimental initiation of haustoria in Agalinis purpurea (Scrophulariaceae). - Amer. J. Bot. 66: 570-575, 1979. Go to original source...
    26. Saslowsky, D., Winkel, B.: Localization of flavonoid enzymes in Arabidopsis roots. - Plant J. 27: 37-48, 2001. Go to original source...
    27. Schenk, R., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous plant cell cultures. - Can. J. Bot. 50: 199-204, 1972. Go to original source...
    28. Tank, D., Egger, J., Olmstead, R.: Phylogenetic classification of subtribe Castillejinae (Orobanchaceae). - Syst. Bot. 34: 182-197, 2009. Go to original source...
    29. Tomilov, A., Tomilova, N., Abdallah, I., Yoder J.: Localized hormone fluxes and early haustorium development in the hemiparasitic plant Triphysaria versicolor. - Plant Physiol. 138: 1469-1480, 2005. Go to original source...
    30. Torres, M., Tomilov, A., Tomilova, N., Reagan, R., Yoder, J.: Pscroph, a parasitic plant EST database enriched for parasite associated transcripts. - BMC Plant Biol. 5: 1-9, 2005. Go to original source...
    31. Trejo-Tapia, G., Rosas-Romero, G., Bermúdez-Torres, K., Zamilpa, A.: In vitro organ cultures of the cancer herb Castilleja tenuiflora Benth. as potential sources of iridoids and antioxidant compounds. - In: Orhan, I. (ed.): Biotechnological Production of Plant Secondary Metabolites. Pp. 87-106. Bentham Science Publishers, Bussum 2012.
    32. Veronesi, C., Bonnin, E., Calvez, S., Thalouarn, P., Simier, P.: Activity of secreted cell wall-modifying enzymes and expression of peroxidase-encoding gene following germination of Orobanche ramose. - Biol. Plant. 51: 391-394, 2007. Go to original source...
    33. Yoder, J.: Host-plant recognition by parasitic Scrophulariaceae. - Curr. Opin. Plant Biol. 4: 359-365, 2001. Go to original source...

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