Biologia plantarum 2016, 60:757-766 | DOI: 10.1007/s10535-016-0651-1

Finding genomic regions and candidate genes governing water use efficiency in rice

V. Roja1, S. Patil1, D. A. Deborah1, A. Srividhya1, N. Ranjitkumar1, G. Kadambari1, P. V. Ramanarao1, E. A. Siddiq1, L. R. Vemireddy1,*
1 Institute of Biotechnology, Acharya NG Ranga Agricultural University, Rajendranagar, Hyderabad, India

Water use efficiency (WUE) is an worth attempting trait to discover the genomic regions governing it, especially in view of the diminishing water resources for the crop plants in general and rice in particular. In order to address this, the present investigation was aimed at identification of genomic regions governing WUE employing a recombinant inbred line population derived from a cross between INRC10192, a high WUE landrace, and IR64, a high yielding cultivar. A total of 36 quantitative trait loci (QTLs) were detected under control as well as drought conditions on chromosomes 1, 2, 4, 8, 9, 10, and 11. Among all, the QTLs with the marker intervals RM486-RM6703, RM6703-RM11484, RM404-RM447, RM24879-RM171, and RM229-RM332 on chromosomes 1, 8, 10, and 11 were found to govern the water use efficiency related traits such as carbon isotope discrimination, specific leaf area, leaf width, and relative water content. Nine major QTL intervals were targeted for candidate gene identification using gene ontology (GO) and transcriptome-based analyses. Overrepresented GO terms in the targeted QTLs were found to be associated with the genes/pathways controlling stomatal regulatory mechanism, stress responsive genes or transcription factors, and saccharide biosynthesis pathways under stress situation. Hence, these genes or genomic regions are potential candidates for development of high WUE rice cultivars.

Keywords: carbon isotope discrimination; QTL mapping; recombinant inbred line; stomatal regulation
Subjects: water use efficiency; carbon isotope discrimination; QTL mapping; recombinant inbred lines; stomata; relative water content; growth analysis; water stress; rice mutants
Species: Oryza sativa

Received: June 25, 2015; Revised: March 31, 2016; Accepted: April 20, 2016; Published: December 1, 2016Show citation

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Roja, V., Patil, S., Deborah, D.A., Srividhya, A., Ranjitkumar, N., Kadambari, G., ... Vemireddy, L.R. (2016). Finding genomic regions and candidate genes governing water use efficiency in rice. Biologia plantarum, 60(4), 757-766. doi: 10.1007/s10535-016-0651-1.
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