Biologia plantarum 2010, 54:231-236 | DOI: 10.1007/s10535-010-0041-z

High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena

Y. Xing1, Y. Yu1, X. Luo1, J. -N. Zhang2, B. Zhao1, Y. -D. Guo1,*
1 College of Agriculture and Biotechnology, China Agricultural University, Beijing, P.R. China
2 Institute of Vegetables, Shanxi Academy of Agricultural Sciences, Taiyuan, P.R. China

A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 zeatin, 0.1 mg dm-3 indoleacetic acid and 10 g dm-3 sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0-2.0 mg dm-3 gibberellic acid and 4.0-8.0 mg dm-3 AgNO3, which promoted shoots elongation. The genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers. The results indicated that almost no somaclonal variation was detected among the regenerants.

Keywords: AgNO3; eggplant; flow cytometry analysis; RAPD; SSR
Subjects: auxins; cytokinins; egg plant; flow cytometry; genetic stability; gibberellic acid, gibberellin; in vitro culture, direct organogenesis; random amplified polymorphic DNA (RAPD); silver nitrate; simple sequence repeats (SSR); sucrose

Received: August 1, 2008; Accepted: April 17, 2009; Published: June 1, 2010Show citation

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Xing, Y., Yu, Y., Luo, X., Zhang, J.-N., Zhao, B., & Guo, Y.-D. (2010). High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena. Biologia plantarum, 54(2), 231-236. doi: 10.1007/s10535-010-0041-z.
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